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PubMed:9516139 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-122 Sentence denotes c-kit is expressed in soft tissue sarcoma of neuroectodermic origin and its ligand prevents apoptosis of neoplastic cells.
TextSentencer_T2 123-288 Sentence denotes During development, mice with mutations of stem cell factor (SCF) or its receptor c-kit exhibit defects in melanogenesis, as well as hematopoiesis and gonadogenesis.
TextSentencer_T3 289-444 Sentence denotes Consequently, accumulating evidence suggests that the c-kit/SCF system plays a crucial role in all of these processes and in tumors which derive from them.
TextSentencer_T4 445-760 Sentence denotes Especially in neuroblastoma (infant tumors of neuroectoderm crest derivation such as melanocytes) it would appear that an autocrine loop exists between c-kit and SCF, and that the functional block of the c-kit receptors with monoclonal antibodies (MoAbs) results in a significant decrease in cellular proliferation.
TextSentencer_T5 761-952 Sentence denotes We studied the expression and role of c-kit and SCF in cell lines of soft tissue sarcoma of neuroectodermic origin, such as Ewing's sarcoma (ES) and peripheral neuro-ectodermal tumors (PNET).
TextSentencer_T6 953-1065 Sentence denotes Using flow cytometry with MoAb CD117 PE, c-kit expression was highlighted in all six of the cell lines examined.
TextSentencer_T7 1066-1258 Sentence denotes This receptor was specifically and functionally activated by SCF, as shown by the binding experiments and the intracellular phosphotyrosine and immunoprecipitation studies that were performed.
TextSentencer_T8 1259-1380 Sentence denotes Using reverse transcriptase polymerase chain reaction analysis, five of the six cellular lines expressed the mRNA of SCF.
TextSentencer_T9 1381-1576 Sentence denotes In the medium measured by using an enzyme- linked immunosorbent assay, low concentrations of SCF were found: only the TC32 cellular line produced significantly higher levels (32 pg) than control.
TextSentencer_T10 1577-1715 Sentence denotes In serum-free culture the addition of SCF reduced the percentage of apoptotic cells from 25% to 90% in five out of the six cellular lines.
TextSentencer_T11 1716-2219 Sentence denotes This observation was confirmed by (1) the functional block of c-kit with MoAb: after 7 days of culture more than 30% of the cells were apoptotic (range 31.5% to 100%) in five out of six cell lines and there was also a decrease in the percentage of cells in phase S, and (2) c-kit antisense oligonucleotides: in the cellular lines treated with oligonucleotides (in relation to the untreated lines) there was a notable reduction (P < .001) both in the absolute number of cells and the 3H-thymidine uptake.
TextSentencer_T12 2220-2365 Sentence denotes These results indicate that ES and PNET express c-kit and its ligand SCF and that SCF is capable of protecting the tumor cells against apoptosis.
TextSentencer_T13 2366-2551 Sentence denotes Furthermore, the reverse transcriptase-polymerase chain reaction performed on the biopsies revealed the presence of mRNA both of SCF and c-kit in practically all of the samples studied.
TextSentencer_T14 2552-2643 Sentence denotes Our in vitro data lead us to assume that SCF may also inhibit tumor cell apoptosis in vivo.
T1 0-122 Sentence denotes c-kit is expressed in soft tissue sarcoma of neuroectodermic origin and its ligand prevents apoptosis of neoplastic cells.
T2 123-288 Sentence denotes During development, mice with mutations of stem cell factor (SCF) or its receptor c-kit exhibit defects in melanogenesis, as well as hematopoiesis and gonadogenesis.
T3 289-444 Sentence denotes Consequently, accumulating evidence suggests that the c-kit/SCF system plays a crucial role in all of these processes and in tumors which derive from them.
T4 445-760 Sentence denotes Especially in neuroblastoma (infant tumors of neuroectoderm crest derivation such as melanocytes) it would appear that an autocrine loop exists between c-kit and SCF, and that the functional block of the c-kit receptors with monoclonal antibodies (MoAbs) results in a significant decrease in cellular proliferation.
T5 761-952 Sentence denotes We studied the expression and role of c-kit and SCF in cell lines of soft tissue sarcoma of neuroectodermic origin, such as Ewing's sarcoma (ES) and peripheral neuro-ectodermal tumors (PNET).
T6 953-1065 Sentence denotes Using flow cytometry with MoAb CD117 PE, c-kit expression was highlighted in all six of the cell lines examined.
T7 1066-1258 Sentence denotes This receptor was specifically and functionally activated by SCF, as shown by the binding experiments and the intracellular phosphotyrosine and immunoprecipitation studies that were performed.
T8 1259-1380 Sentence denotes Using reverse transcriptase polymerase chain reaction analysis, five of the six cellular lines expressed the mRNA of SCF.
T9 1381-1576 Sentence denotes In the medium measured by using an enzyme- linked immunosorbent assay, low concentrations of SCF were found: only the TC32 cellular line produced significantly higher levels (32 pg) than control.
T10 1577-1715 Sentence denotes In serum-free culture the addition of SCF reduced the percentage of apoptotic cells from 25% to 90% in five out of the six cellular lines.
T11 1716-2219 Sentence denotes This observation was confirmed by (1) the functional block of c-kit with MoAb: after 7 days of culture more than 30% of the cells were apoptotic (range 31.5% to 100%) in five out of six cell lines and there was also a decrease in the percentage of cells in phase S, and (2) c-kit antisense oligonucleotides: in the cellular lines treated with oligonucleotides (in relation to the untreated lines) there was a notable reduction (P < .001) both in the absolute number of cells and the 3H-thymidine uptake.
T12 2220-2365 Sentence denotes These results indicate that ES and PNET express c-kit and its ligand SCF and that SCF is capable of protecting the tumor cells against apoptosis.
T13 2366-2551 Sentence denotes Furthermore, the reverse transcriptase-polymerase chain reaction performed on the biopsies revealed the presence of mRNA both of SCF and c-kit in practically all of the samples studied.
T14 2552-2643 Sentence denotes Our in vitro data lead us to assume that SCF may also inhibit tumor cell apoptosis in vivo.

DisGeNET

Id Subject Object Predicate Lexical cue
T0 649-654 gene:3815 denotes c-kit
T1 459-472 disease:C0027819 denotes neuroblastoma
T2 649-654 gene:3815 denotes c-kit
T3 459-472 disease:C0700095 denotes neuroblastoma
R1 T0 T1 associated_with c-kit,neuroblastoma
R2 T2 T3 associated_with c-kit,neuroblastoma

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 1580-1585 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
PD-UBERON-AE-B_T2 27-33 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
PD-UBERON-AE-B_T3 835-841 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
PD-UBERON-AE-B_T4 505-510 http://purl.obolibrary.org/obo/UBERON_4200133 denotes crest
PD-UBERON-AE-B_T5 927-937 http://purl.obolibrary.org/obo/UBERON_0000924 denotes ectodermal

DisGeNet-2017-sample

Id Subject Object Predicate Lexical cue
T1256 649-654 gene:3815 denotes c-kit
T1257 459-472 disease:C0027819 denotes neuroblastoma
R1 T1256 T1257 associated_with c-kit,neuroblastoma
R2 T1256 T1257 associated_with c-kit,neuroblastoma

UBERON-AE

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 27-33 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
PD-UBERON-AE-B_T2 835-841 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
PD-UBERON-AE-B_T3 505-510 http://purl.obolibrary.org/obo/UBERON_4200133 denotes crest
PD-UBERON-AE-B_T4 927-937 http://purl.obolibrary.org/obo/UBERON_0000924 denotes ectodermal
PD-UBERON-AE-B_T5 1580-1585 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum