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GlyCosmos6-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 234-241 Body_part denotes surface http://purl.obolibrary.org/obo/UBERON_0002416

Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 327-338 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T2 535-546 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T3 601-613 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acids
T4 856-867 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T5 1305-1316 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T6 1643-1667 https://glytoucan.org/Structures/Glycans/G50850NI denotes N-acetyl-neuraminic acid
T7 1643-1667 https://glytoucan.org/Structures/Glycans/G81533KY denotes N-acetyl-neuraminic acid
T8 2188-2199 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 327-338 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T2 535-546 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T3 601-613 Glycan_Motif denotes sialic acids https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T4 856-867 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T5 1305-1316 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T6 1643-1667 Glycan_Motif denotes N-acetyl-neuraminic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI
T8 2188-2199 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 327-338 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T2 535-546 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T3 601-613 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acids
T4 856-867 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T5 1305-1316 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T6 1643-1667 https://glytoucan.org/Structures/Glycans/G50850NI denotes N-acetyl-neuraminic acid
T7 1643-1667 https://glytoucan.org/Structures/Glycans/G81533KY denotes N-acetyl-neuraminic acid
T8 2188-2199 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-168 Sentence denotes Temperature differences for trans-glycosylation and hydrolysis reaction reveal an acceptor binding site in the catalytic mechanism of Trypanosoma cruzi trans-sialidase.
TextSentencer_T2 169-467 Sentence denotes Trypanosoma cruzi, the agent of Chagas disease, expresses on its surface a trans-sialidase that catalyzes preferentially the transference of alpha-2,3-linked sialic acid to acceptors containing terminal beta-galactosyl residues, instead of the typical hydrolysis reaction, found in most sialidases.
TextSentencer_T3 468-614 Sentence denotes The trans-sialidase is responsible for the acquisition of the host sialic acid by this protozoan parasite, which does not synthesize sialic acids.
TextSentencer_T4 615-724 Sentence denotes Here, we have studied some kinetic properties of a recombinant trans-sialidase expressed in Escherichia coli.
TextSentencer_T5 725-842 Sentence denotes We found that it has sequential-type kinetics for the transferase reaction, as shown for the parasite-derived enzyme.
TextSentencer_T6 843-996 Sentence denotes The rates of sialic acid transfer to water (hydrolysis), and to beta-galactosyl residues have a unique behavior with respect to the reaction temperature.
TextSentencer_T7 997-1181 Sentence denotes While the hydrolysis rate of sialyllactose increases continuously up to 35 degrees C, the temperature for the maximal rate of trans-glycosylation depends on the acceptor concentration.
TextSentencer_T8 1182-1324 Sentence denotes At low acceptor concentrations the rate of trans-glycosylation is maximal at 13 degrees C and independent of the amount of sialic acid donors.
TextSentencer_T9 1325-1438 Sentence denotes With increasing acceptor concentrations, maximal rates of trans-glycosylation are shifted to higher temperatures.
TextSentencer_T10 1439-1548 Sentence denotes This finding is explained by an 8-fold increase in the Km for the acceptor from 13 degrees C to 33 degrees C.
TextSentencer_T11 1549-1668 Sentence denotes Differences in hydrolysis and transfer rates were also obtained by using 4-methylumbelliferyl-N-acetyl-neuraminic acid.
TextSentencer_T12 1669-1827 Sentence denotes However, its hydrolysis rate is much higher than the rate of transference to lactose, suggesting that a long-lived enzyme-sialosyl intermediate is not formed.
TextSentencer_T13 1828-1990 Sentence denotes In addition, lactose does not increase the rate of methyl-umbelliferone release at any temperature, indicating that the rate limiting step is the aglycon release.
TextSentencer_T14 1991-2227 Sentence denotes Based on these results we propose that transglycosylation in T. cruzi sialidase is favored by the existence of a binding site for beta-galactosyl residues, which accepts the new glycosidic bond as sialic acid is released from the donor.
TextSentencer_T15 2228-2442 Sentence denotes With increasing temperature the affinity for the acceptor decreases, resulting in a concomitant increase in the rate of transfer to water, which, in turn, can be suppressed by increasing the acceptor concentration.
T1 0-168 Sentence denotes Temperature differences for trans-glycosylation and hydrolysis reaction reveal an acceptor binding site in the catalytic mechanism of Trypanosoma cruzi trans-sialidase.
T2 169-467 Sentence denotes Trypanosoma cruzi, the agent of Chagas disease, expresses on its surface a trans-sialidase that catalyzes preferentially the transference of alpha-2,3-linked sialic acid to acceptors containing terminal beta-galactosyl residues, instead of the typical hydrolysis reaction, found in most sialidases.
T3 468-614 Sentence denotes The trans-sialidase is responsible for the acquisition of the host sialic acid by this protozoan parasite, which does not synthesize sialic acids.
T4 615-724 Sentence denotes Here, we have studied some kinetic properties of a recombinant trans-sialidase expressed in Escherichia coli.
T5 725-842 Sentence denotes We found that it has sequential-type kinetics for the transferase reaction, as shown for the parasite-derived enzyme.
T6 843-996 Sentence denotes The rates of sialic acid transfer to water (hydrolysis), and to beta-galactosyl residues have a unique behavior with respect to the reaction temperature.
T7 997-1181 Sentence denotes While the hydrolysis rate of sialyllactose increases continuously up to 35 degrees C, the temperature for the maximal rate of trans-glycosylation depends on the acceptor concentration.
T8 1182-1324 Sentence denotes At low acceptor concentrations the rate of trans-glycosylation is maximal at 13 degrees C and independent of the amount of sialic acid donors.
T9 1325-1438 Sentence denotes With increasing acceptor concentrations, maximal rates of trans-glycosylation are shifted to higher temperatures.
T10 1439-1668 Sentence denotes This finding is explained by an 8-fold increase in the Km for the acceptor from 13 degrees C to 33 degrees C. Differences in hydrolysis and transfer rates were also obtained by using 4-methylumbelliferyl-N-acetyl-neuraminic acid.
T11 1669-1827 Sentence denotes However, its hydrolysis rate is much higher than the rate of transference to lactose, suggesting that a long-lived enzyme-sialosyl intermediate is not formed.
T12 1828-1990 Sentence denotes In addition, lactose does not increase the rate of methyl-umbelliferone release at any temperature, indicating that the rate limiting step is the aglycon release.
T13 1991-2227 Sentence denotes Based on these results we propose that transglycosylation in T. cruzi sialidase is favored by the existence of a binding site for beta-galactosyl residues, which accepts the new glycosidic bond as sialic acid is released from the donor.
T14 2228-2442 Sentence denotes With increasing temperature the affinity for the acceptor decreases, resulting in a concomitant increase in the rate of transfer to water, which, in turn, can be suppressed by increasing the acceptor concentration.
T1 0-168 Sentence denotes Temperature differences for trans-glycosylation and hydrolysis reaction reveal an acceptor binding site in the catalytic mechanism of Trypanosoma cruzi trans-sialidase.
T2 169-467 Sentence denotes Trypanosoma cruzi, the agent of Chagas disease, expresses on its surface a trans-sialidase that catalyzes preferentially the transference of alpha-2,3-linked sialic acid to acceptors containing terminal beta-galactosyl residues, instead of the typical hydrolysis reaction, found in most sialidases.
T3 468-614 Sentence denotes The trans-sialidase is responsible for the acquisition of the host sialic acid by this protozoan parasite, which does not synthesize sialic acids.
T4 615-724 Sentence denotes Here, we have studied some kinetic properties of a recombinant trans-sialidase expressed in Escherichia coli.
T5 725-842 Sentence denotes We found that it has sequential-type kinetics for the transferase reaction, as shown for the parasite-derived enzyme.
T6 843-996 Sentence denotes The rates of sialic acid transfer to water (hydrolysis), and to beta-galactosyl residues have a unique behavior with respect to the reaction temperature.
T7 997-1181 Sentence denotes While the hydrolysis rate of sialyllactose increases continuously up to 35 degrees C, the temperature for the maximal rate of trans-glycosylation depends on the acceptor concentration.
T8 1182-1324 Sentence denotes At low acceptor concentrations the rate of trans-glycosylation is maximal at 13 degrees C and independent of the amount of sialic acid donors.
T9 1325-1438 Sentence denotes With increasing acceptor concentrations, maximal rates of trans-glycosylation are shifted to higher temperatures.
T10 1439-1548 Sentence denotes This finding is explained by an 8-fold increase in the Km for the acceptor from 13 degrees C to 33 degrees C.
T11 1549-1668 Sentence denotes Differences in hydrolysis and transfer rates were also obtained by using 4-methylumbelliferyl-N-acetyl-neuraminic acid.
T12 1669-1827 Sentence denotes However, its hydrolysis rate is much higher than the rate of transference to lactose, suggesting that a long-lived enzyme-sialosyl intermediate is not formed.
T13 1828-1990 Sentence denotes In addition, lactose does not increase the rate of methyl-umbelliferone release at any temperature, indicating that the rate limiting step is the aglycon release.
T14 1991-2227 Sentence denotes Based on these results we propose that transglycosylation in T. cruzi sialidase is favored by the existence of a binding site for beta-galactosyl residues, which accepts the new glycosidic bond as sialic acid is released from the donor.
T15 2228-2442 Sentence denotes With increasing temperature the affinity for the acceptor decreases, resulting in a concomitant increase in the rate of transfer to water, which, in turn, can be suppressed by increasing the acceptor concentration.

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 201-215 Disease denotes Chagas disease http://purl.obolibrary.org/obo/MONDO_0001444

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 134-151 OrganismTaxon denotes Trypanosoma cruzi NCBItxid:5693
T2 169-186 OrganismTaxon denotes Trypanosoma cruzi NCBItxid:5693
T3 707-723 OrganismTaxon denotes Escherichia coli NCBItxid:562

GlycoBiology-PACDB

Id Subject Object Predicate Lexical cue
_T1 134-151 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC655 denotes Trypanosoma cruzi
_T2 169-186 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC655 denotes Trypanosoma cruzi
_T3 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 denotes Escherichia coli
_T4 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 denotes Escherichia coli
_T5 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 denotes Escherichia coli
_T6 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 denotes Escherichia coli
_T7 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 denotes Escherichia coli
_T8 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 denotes Escherichia coli
_T9 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 denotes Escherichia coli
_T10 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 denotes Escherichia coli
_T11 707-723 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 denotes Escherichia coli

ICD10

Id Subject Object Predicate Lexical cue
T1 201-215 http://purl.bioontology.org/ontology/ICD10/B57 denotes Chagas disease

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 234-241 FMAID:50594 denotes surface
_T2 234-241 FMAID:146300 denotes surface
_T3 847-852 FMAID:217859 denotes rates
_T4 1018-1022 FMAID:217859 denotes rate
_T5 1115-1119 FMAID:217859 denotes rate
_T6 1217-1221 FMAID:217859 denotes rate
_T7 1374-1379 FMAID:217859 denotes rates
_T8 1588-1593 FMAID:217859 denotes rates
_T9 1643-1667 FMAID:196782 denotes N-acetyl-neuraminic acid
_T10 1643-1667 FMAID:82788 denotes N-acetyl-neuraminic acid
_T11 1693-1697 FMAID:217859 denotes rate
_T12 1722-1726 FMAID:217859 denotes rate
_T13 1800-1812 FMAID:74531 denotes intermediate
_T14 1800-1812 FMAID:179268 denotes intermediate
_T15 1871-1875 FMAID:217859 denotes rate
_T16 1948-1952 FMAID:217859 denotes rate
_T17 2340-2344 FMAID:217859 denotes rate

uniprot-human

Id Subject Object Predicate Lexical cue
T1 779-790 http://www.uniprot.org/uniprot/Q99484 denotes transferase

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 779-790 http://www.uniprot.org/uniprot/P38649 denotes transferase

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 121-130 http://purl.bioontology.org/ontology/NCBITAXON/127244 denotes mechanism
T2 134-145 http://purl.bioontology.org/ontology/NCBITAXON/5654 denotes Trypanosoma
T3 134-145 http://purl.bioontology.org/ontology/NCBITAXON/5690 denotes Trypanosoma
T4 134-145 http://purl.bioontology.org/ontology/NCBITAXON/71805 denotes Trypanosoma
T5 134-145 http://purl.bioontology.org/ontology/NCBITAXON/56869 denotes Trypanosoma
T6 169-180 http://purl.bioontology.org/ontology/NCBITAXON/5654 denotes Trypanosoma
T7 169-180 http://purl.bioontology.org/ontology/NCBITAXON/71805 denotes Trypanosoma
T8 169-180 http://purl.bioontology.org/ontology/NCBITAXON/56869 denotes Trypanosoma
T9 169-180 http://purl.bioontology.org/ontology/NCBITAXON/5690 denotes Trypanosoma
T10 372-376 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T11 372-376 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T12 565-573 http://purl.bioontology.org/ontology/NCBITAXON/600794 denotes parasite
T13 707-718 http://purl.bioontology.org/ontology/NCBITAXON/561 denotes Escherichia
T14 818-826 http://purl.bioontology.org/ontology/NCBITAXON/600794 denotes parasite
T15 907-911 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T16 907-911 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T17 946-954 http://purl.bioontology.org/ontology/STY/T053 denotes behavior
T18 1444-1451 http://purl.bioontology.org/ontology/STY/T033 denotes finding
T19 2121-2125 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T20 2121-2125 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta

GO-BP

Id Subject Object Predicate Lexical cue
T1 34-47 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T2 1129-1142 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T3 1231-1244 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T4 1389-1402 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T5 565-573 http://purl.obolibrary.org/obo/GO_0072519 denotes parasite
T6 818-826 http://purl.obolibrary.org/obo/GO_0072519 denotes parasite
T7 946-954 http://purl.obolibrary.org/obo/GO_0007610 denotes behavior
T8 1879-1885 http://purl.obolibrary.org/obo/GO_0032259 denotes methyl

GO-MF

Id Subject Object Predicate Lexical cue
T1 91-98 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T2 2104-2111 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T3 91-98 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T4 2104-2111 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T5 91-98 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T6 2104-2111 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T7 91-98 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T8 2104-2111 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-CC

Id Subject Object Predicate Lexical cue
T1 530-534 http://purl.obolibrary.org/obo/GO_0018995 denotes host