PubMed:9455925
Annnotations
GlyCosmos6-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 234-241 | Body_part | denotes | surface | http://purl.obolibrary.org/obo/UBERON_0002416 |
Glycan-Motif
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 327-338 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T2 | 535-546 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T3 | 601-613 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acids |
T4 | 856-867 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T5 | 1305-1316 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T6 | 1643-1667 | https://glytoucan.org/Structures/Glycans/G50850NI | denotes | N-acetyl-neuraminic acid |
T7 | 1643-1667 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | N-acetyl-neuraminic acid |
T8 | 2188-2199 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
GlyCosmos6-Glycan-Motif-Image
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 327-338 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
T2 | 535-546 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
T3 | 601-613 | Glycan_Motif | denotes | sialic acids | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
T4 | 856-867 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
T5 | 1305-1316 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
T6 | 1643-1667 | Glycan_Motif | denotes | N-acetyl-neuraminic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI |
T8 | 2188-2199 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
GlyCosmos6-Glycan-Motif-Structure
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 327-338 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T2 | 535-546 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T3 | 601-613 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acids |
T4 | 856-867 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T5 | 1305-1316 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T6 | 1643-1667 | https://glytoucan.org/Structures/Glycans/G50850NI | denotes | N-acetyl-neuraminic acid |
T7 | 1643-1667 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | N-acetyl-neuraminic acid |
T8 | 2188-2199 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
TextSentencer_T1 | 0-168 | Sentence | denotes | Temperature differences for trans-glycosylation and hydrolysis reaction reveal an acceptor binding site in the catalytic mechanism of Trypanosoma cruzi trans-sialidase. |
TextSentencer_T2 | 169-467 | Sentence | denotes | Trypanosoma cruzi, the agent of Chagas disease, expresses on its surface a trans-sialidase that catalyzes preferentially the transference of alpha-2,3-linked sialic acid to acceptors containing terminal beta-galactosyl residues, instead of the typical hydrolysis reaction, found in most sialidases. |
TextSentencer_T3 | 468-614 | Sentence | denotes | The trans-sialidase is responsible for the acquisition of the host sialic acid by this protozoan parasite, which does not synthesize sialic acids. |
TextSentencer_T4 | 615-724 | Sentence | denotes | Here, we have studied some kinetic properties of a recombinant trans-sialidase expressed in Escherichia coli. |
TextSentencer_T5 | 725-842 | Sentence | denotes | We found that it has sequential-type kinetics for the transferase reaction, as shown for the parasite-derived enzyme. |
TextSentencer_T6 | 843-996 | Sentence | denotes | The rates of sialic acid transfer to water (hydrolysis), and to beta-galactosyl residues have a unique behavior with respect to the reaction temperature. |
TextSentencer_T7 | 997-1181 | Sentence | denotes | While the hydrolysis rate of sialyllactose increases continuously up to 35 degrees C, the temperature for the maximal rate of trans-glycosylation depends on the acceptor concentration. |
TextSentencer_T8 | 1182-1324 | Sentence | denotes | At low acceptor concentrations the rate of trans-glycosylation is maximal at 13 degrees C and independent of the amount of sialic acid donors. |
TextSentencer_T9 | 1325-1438 | Sentence | denotes | With increasing acceptor concentrations, maximal rates of trans-glycosylation are shifted to higher temperatures. |
TextSentencer_T10 | 1439-1548 | Sentence | denotes | This finding is explained by an 8-fold increase in the Km for the acceptor from 13 degrees C to 33 degrees C. |
TextSentencer_T11 | 1549-1668 | Sentence | denotes | Differences in hydrolysis and transfer rates were also obtained by using 4-methylumbelliferyl-N-acetyl-neuraminic acid. |
TextSentencer_T12 | 1669-1827 | Sentence | denotes | However, its hydrolysis rate is much higher than the rate of transference to lactose, suggesting that a long-lived enzyme-sialosyl intermediate is not formed. |
TextSentencer_T13 | 1828-1990 | Sentence | denotes | In addition, lactose does not increase the rate of methyl-umbelliferone release at any temperature, indicating that the rate limiting step is the aglycon release. |
TextSentencer_T14 | 1991-2227 | Sentence | denotes | Based on these results we propose that transglycosylation in T. cruzi sialidase is favored by the existence of a binding site for beta-galactosyl residues, which accepts the new glycosidic bond as sialic acid is released from the donor. |
TextSentencer_T15 | 2228-2442 | Sentence | denotes | With increasing temperature the affinity for the acceptor decreases, resulting in a concomitant increase in the rate of transfer to water, which, in turn, can be suppressed by increasing the acceptor concentration. |
T1 | 0-168 | Sentence | denotes | Temperature differences for trans-glycosylation and hydrolysis reaction reveal an acceptor binding site in the catalytic mechanism of Trypanosoma cruzi trans-sialidase. |
T2 | 169-467 | Sentence | denotes | Trypanosoma cruzi, the agent of Chagas disease, expresses on its surface a trans-sialidase that catalyzes preferentially the transference of alpha-2,3-linked sialic acid to acceptors containing terminal beta-galactosyl residues, instead of the typical hydrolysis reaction, found in most sialidases. |
T3 | 468-614 | Sentence | denotes | The trans-sialidase is responsible for the acquisition of the host sialic acid by this protozoan parasite, which does not synthesize sialic acids. |
T4 | 615-724 | Sentence | denotes | Here, we have studied some kinetic properties of a recombinant trans-sialidase expressed in Escherichia coli. |
T5 | 725-842 | Sentence | denotes | We found that it has sequential-type kinetics for the transferase reaction, as shown for the parasite-derived enzyme. |
T6 | 843-996 | Sentence | denotes | The rates of sialic acid transfer to water (hydrolysis), and to beta-galactosyl residues have a unique behavior with respect to the reaction temperature. |
T7 | 997-1181 | Sentence | denotes | While the hydrolysis rate of sialyllactose increases continuously up to 35 degrees C, the temperature for the maximal rate of trans-glycosylation depends on the acceptor concentration. |
T8 | 1182-1324 | Sentence | denotes | At low acceptor concentrations the rate of trans-glycosylation is maximal at 13 degrees C and independent of the amount of sialic acid donors. |
T9 | 1325-1438 | Sentence | denotes | With increasing acceptor concentrations, maximal rates of trans-glycosylation are shifted to higher temperatures. |
T10 | 1439-1668 | Sentence | denotes | This finding is explained by an 8-fold increase in the Km for the acceptor from 13 degrees C to 33 degrees C. Differences in hydrolysis and transfer rates were also obtained by using 4-methylumbelliferyl-N-acetyl-neuraminic acid. |
T11 | 1669-1827 | Sentence | denotes | However, its hydrolysis rate is much higher than the rate of transference to lactose, suggesting that a long-lived enzyme-sialosyl intermediate is not formed. |
T12 | 1828-1990 | Sentence | denotes | In addition, lactose does not increase the rate of methyl-umbelliferone release at any temperature, indicating that the rate limiting step is the aglycon release. |
T13 | 1991-2227 | Sentence | denotes | Based on these results we propose that transglycosylation in T. cruzi sialidase is favored by the existence of a binding site for beta-galactosyl residues, which accepts the new glycosidic bond as sialic acid is released from the donor. |
T14 | 2228-2442 | Sentence | denotes | With increasing temperature the affinity for the acceptor decreases, resulting in a concomitant increase in the rate of transfer to water, which, in turn, can be suppressed by increasing the acceptor concentration. |
T1 | 0-168 | Sentence | denotes | Temperature differences for trans-glycosylation and hydrolysis reaction reveal an acceptor binding site in the catalytic mechanism of Trypanosoma cruzi trans-sialidase. |
T2 | 169-467 | Sentence | denotes | Trypanosoma cruzi, the agent of Chagas disease, expresses on its surface a trans-sialidase that catalyzes preferentially the transference of alpha-2,3-linked sialic acid to acceptors containing terminal beta-galactosyl residues, instead of the typical hydrolysis reaction, found in most sialidases. |
T3 | 468-614 | Sentence | denotes | The trans-sialidase is responsible for the acquisition of the host sialic acid by this protozoan parasite, which does not synthesize sialic acids. |
T4 | 615-724 | Sentence | denotes | Here, we have studied some kinetic properties of a recombinant trans-sialidase expressed in Escherichia coli. |
T5 | 725-842 | Sentence | denotes | We found that it has sequential-type kinetics for the transferase reaction, as shown for the parasite-derived enzyme. |
T6 | 843-996 | Sentence | denotes | The rates of sialic acid transfer to water (hydrolysis), and to beta-galactosyl residues have a unique behavior with respect to the reaction temperature. |
T7 | 997-1181 | Sentence | denotes | While the hydrolysis rate of sialyllactose increases continuously up to 35 degrees C, the temperature for the maximal rate of trans-glycosylation depends on the acceptor concentration. |
T8 | 1182-1324 | Sentence | denotes | At low acceptor concentrations the rate of trans-glycosylation is maximal at 13 degrees C and independent of the amount of sialic acid donors. |
T9 | 1325-1438 | Sentence | denotes | With increasing acceptor concentrations, maximal rates of trans-glycosylation are shifted to higher temperatures. |
T10 | 1439-1548 | Sentence | denotes | This finding is explained by an 8-fold increase in the Km for the acceptor from 13 degrees C to 33 degrees C. |
T11 | 1549-1668 | Sentence | denotes | Differences in hydrolysis and transfer rates were also obtained by using 4-methylumbelliferyl-N-acetyl-neuraminic acid. |
T12 | 1669-1827 | Sentence | denotes | However, its hydrolysis rate is much higher than the rate of transference to lactose, suggesting that a long-lived enzyme-sialosyl intermediate is not formed. |
T13 | 1828-1990 | Sentence | denotes | In addition, lactose does not increase the rate of methyl-umbelliferone release at any temperature, indicating that the rate limiting step is the aglycon release. |
T14 | 1991-2227 | Sentence | denotes | Based on these results we propose that transglycosylation in T. cruzi sialidase is favored by the existence of a binding site for beta-galactosyl residues, which accepts the new glycosidic bond as sialic acid is released from the donor. |
T15 | 2228-2442 | Sentence | denotes | With increasing temperature the affinity for the acceptor decreases, resulting in a concomitant increase in the rate of transfer to water, which, in turn, can be suppressed by increasing the acceptor concentration. |
mondo_disease
Id | Subject | Object | Predicate | Lexical cue | mondo_id |
---|---|---|---|---|---|
T1 | 201-215 | Disease | denotes | Chagas disease | http://purl.obolibrary.org/obo/MONDO_0001444 |
NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 134-151 | OrganismTaxon | denotes | Trypanosoma cruzi | NCBItxid:5693 |
T2 | 169-186 | OrganismTaxon | denotes | Trypanosoma cruzi | NCBItxid:5693 |
T3 | 707-723 | OrganismTaxon | denotes | Escherichia coli | NCBItxid:562 |
GlycoBiology-PACDB
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
_T1 | 134-151 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC655 | denotes | Trypanosoma cruzi |
_T2 | 169-186 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC655 | denotes | Trypanosoma cruzi |
_T3 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 | denotes | Escherichia coli |
_T4 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 | denotes | Escherichia coli |
_T5 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 | denotes | Escherichia coli |
_T6 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 | denotes | Escherichia coli |
_T7 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 | denotes | Escherichia coli |
_T8 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 | denotes | Escherichia coli |
_T9 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 | denotes | Escherichia coli |
_T10 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 | denotes | Escherichia coli |
_T11 | 707-723 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 | denotes | Escherichia coli |
ICD10
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 201-215 | http://purl.bioontology.org/ontology/ICD10/B57 | denotes | Chagas disease |
GlycoBiology-FMA
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
_T1 | 234-241 | FMAID:50594 | denotes | surface |
_T2 | 234-241 | FMAID:146300 | denotes | surface |
_T3 | 847-852 | FMAID:217859 | denotes | rates |
_T4 | 1018-1022 | FMAID:217859 | denotes | rate |
_T5 | 1115-1119 | FMAID:217859 | denotes | rate |
_T6 | 1217-1221 | FMAID:217859 | denotes | rate |
_T7 | 1374-1379 | FMAID:217859 | denotes | rates |
_T8 | 1588-1593 | FMAID:217859 | denotes | rates |
_T9 | 1643-1667 | FMAID:196782 | denotes | N-acetyl-neuraminic acid |
_T10 | 1643-1667 | FMAID:82788 | denotes | N-acetyl-neuraminic acid |
_T11 | 1693-1697 | FMAID:217859 | denotes | rate |
_T12 | 1722-1726 | FMAID:217859 | denotes | rate |
_T13 | 1800-1812 | FMAID:74531 | denotes | intermediate |
_T14 | 1800-1812 | FMAID:179268 | denotes | intermediate |
_T15 | 1871-1875 | FMAID:217859 | denotes | rate |
_T16 | 1948-1952 | FMAID:217859 | denotes | rate |
_T17 | 2340-2344 | FMAID:217859 | denotes | rate |
uniprot-human
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 779-790 | http://www.uniprot.org/uniprot/Q99484 | denotes | transferase |
uniprot-mouse
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 779-790 | http://www.uniprot.org/uniprot/P38649 | denotes | transferase |
GlycoBiology-NCBITAXON
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 121-130 | http://purl.bioontology.org/ontology/NCBITAXON/127244 | denotes | mechanism |
T2 | 134-145 | http://purl.bioontology.org/ontology/NCBITAXON/5654 | denotes | Trypanosoma |
T3 | 134-145 | http://purl.bioontology.org/ontology/NCBITAXON/5690 | denotes | Trypanosoma |
T4 | 134-145 | http://purl.bioontology.org/ontology/NCBITAXON/71805 | denotes | Trypanosoma |
T5 | 134-145 | http://purl.bioontology.org/ontology/NCBITAXON/56869 | denotes | Trypanosoma |
T6 | 169-180 | http://purl.bioontology.org/ontology/NCBITAXON/5654 | denotes | Trypanosoma |
T7 | 169-180 | http://purl.bioontology.org/ontology/NCBITAXON/71805 | denotes | Trypanosoma |
T8 | 169-180 | http://purl.bioontology.org/ontology/NCBITAXON/56869 | denotes | Trypanosoma |
T9 | 169-180 | http://purl.bioontology.org/ontology/NCBITAXON/5690 | denotes | Trypanosoma |
T10 | 372-376 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
T11 | 372-376 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
T12 | 565-573 | http://purl.bioontology.org/ontology/NCBITAXON/600794 | denotes | parasite |
T13 | 707-718 | http://purl.bioontology.org/ontology/NCBITAXON/561 | denotes | Escherichia |
T14 | 818-826 | http://purl.bioontology.org/ontology/NCBITAXON/600794 | denotes | parasite |
T15 | 907-911 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
T16 | 907-911 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
T17 | 946-954 | http://purl.bioontology.org/ontology/STY/T053 | denotes | behavior |
T18 | 1444-1451 | http://purl.bioontology.org/ontology/STY/T033 | denotes | finding |
T19 | 2121-2125 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
T20 | 2121-2125 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 34-47 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
T2 | 1129-1142 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
T3 | 1231-1244 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
T4 | 1389-1402 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
T5 | 565-573 | http://purl.obolibrary.org/obo/GO_0072519 | denotes | parasite |
T6 | 818-826 | http://purl.obolibrary.org/obo/GO_0072519 | denotes | parasite |
T7 | 946-954 | http://purl.obolibrary.org/obo/GO_0007610 | denotes | behavior |
T8 | 1879-1885 | http://purl.obolibrary.org/obo/GO_0032259 | denotes | methyl |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 91-98 | http://purl.obolibrary.org/obo/GO_0070026 | denotes | binding |
T2 | 2104-2111 | http://purl.obolibrary.org/obo/GO_0070026 | denotes | binding |
T3 | 91-98 | http://purl.obolibrary.org/obo/GO_0003680 | denotes | binding |
T4 | 2104-2111 | http://purl.obolibrary.org/obo/GO_0003680 | denotes | binding |
T5 | 91-98 | http://purl.obolibrary.org/obo/GO_0017091 | denotes | binding |
T6 | 2104-2111 | http://purl.obolibrary.org/obo/GO_0017091 | denotes | binding |
T7 | 91-98 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | binding |
T8 | 2104-2111 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | binding |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 530-534 | http://purl.obolibrary.org/obo/GO_0018995 | denotes | host |