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PubMed:9452454 JSONTXT

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Id Subject Object Predicate Lexical cue
T1 0-95 Sentence denotes The I domain of integrin LFA-1 interacts with ICAM-1 domain 1 at residue Glu-34 but not Gln-73.
T2 96-254 Sentence denotes Using a solid phase assay, we show that isolated LFA-1 I domain binds ICAM-1 in a Mg2+-dependent manner and is blocked by anti-I domain monoclonal antibodies.
T3 255-368 Sentence denotes This activity mirrors that of the intact receptor (Dransfield, I., Cabañas, C., Craig, A., and Hogg, N. (1992) J.
T4 369-379 Sentence denotes Cell Biol.
T5 380-478 Sentence denotes 116, 219-226) and suggests that the I domain controls divalent cation-dependent receptor function.
T6 479-615 Sentence denotes In ICAM-1, domain 1 residues Glu-34 and Gln-73 have been identified as critical for binding of LFA-1 as an intact receptor (Staunton, D.
T7 616-630 Sentence denotes E., Dustin, M.
T8 631-647 Sentence denotes L., Erickson, H.
T9 648-668 Sentence denotes P., and Springer, T.
T10 669-697 Sentence denotes A. (1990) Cell 61, 243-254).
T11 698-866 Sentence denotes For the first time, we show that isolated I domain binds to domain 1 of ICAM-1 and that this interaction is inhibited partially by mutation of Glu-34 but not by Gln-73.
T12 867-944 Sentence denotes The anti-ICAM-1 monoclonal antibody RR1/1, which maps to Gln-73 (Staunton, D.
T13 945-959 Sentence denotes E., Dustin, M.
T14 960-976 Sentence denotes L., Erickson, H.
T15 977-997 Sentence denotes P., and Springer, T.
T16 998-1131 Sentence denotes A. (1990) Cell 61, 243-254), enhances I domain binding, suggesting potential allosteric control or coordinate binding by this region.
T17 1132-1337 Sentence denotes Finally, I domain binding inhibited by Glu-34 ICAM-1 mutation correlates with divalent cation dependence, indicating that this residue might be in direct contact with the metal ion-dependent adhesion site.
T18 1338-1529 Sentence denotes Thus, we describe the interaction between the LFA-1 I domain and ICAM-1, an event that controls the function of the intact receptor but includes only part of the complete ligand binding site.