| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-150 |
Sentence |
denotes |
Progressive cyclic nucleotide-induced conformational changes in the cGMP-dependent protein kinase studied by small angle X-ray scattering in solution. |
| T2 |
151-408 |
Sentence |
denotes |
Small angle scattering data from bovine lung type Ialpha cGMP-dependent protein kinase (PKG) in the absence of cGMP show the protein to have a highly asymmetric structure with a radius of gyration (Rg) of 45 A and a maximum linear dimension (dmax) of 165 A. |
| T3 |
409-476 |
Sentence |
denotes |
The addition of cGMP induces a marked conformational change in PKG. |
| T4 |
477-627 |
Sentence |
denotes |
The Rg and dmax increase 25-30%, and the protein's mass moves further away from the center of mass; this results in an even more asymmetric structure. |
| T5 |
628-903 |
Sentence |
denotes |
Fourier transform infrared spectroscopy data suggest that the conformational change induced by cGMP binding is primarily due to a topographical movement of the structural domains of PKG rather than to secondary structural changes within one or more of the individual domains. |
| T6 |
904-993 |
Sentence |
denotes |
Each monomer of the dimeric PKG contains one high and one low affinity cGMP-binding site. |
| T7 |
994-1174 |
Sentence |
denotes |
A prominent increase in the asymmetry of PKG occurs with binding to high affinity cGMP-binding sites alone, but the full domain movements require the binding to both sets of sites. |
| T8 |
1175-1430 |
Sentence |
denotes |
These conformational changes occurring in PKG with the progressive binding of cGMP to both sets of cGMP-binding sites correlate with past data, which have indicated that cGMP binding to both sets of sites is required for the full activation of the enzyme. |
| T9 |
1431-1663 |
Sentence |
denotes |
These results provide the first quantitative measurement of the overall PKG structure, as well as an assessment of the structural events that accompany the activation of a protein kinase upon binding a small molecular weight ligand. |
