PubMed:9356353 JSONTXT

Annnotations TAB JSON ListView MergeView

    jnlpba-st-training

    {"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":0,"end":8},"obj":"protein"},{"id":"T2","span":{"begin":29,"end":45},"obj":"protein"},{"id":"T3","span":{"begin":82,"end":91},"obj":"DNA"},{"id":"T4","span":{"begin":121,"end":152},"obj":"DNA"},{"id":"T5","span":{"begin":154,"end":174},"obj":"protein"},{"id":"T6","span":{"begin":176,"end":180},"obj":"protein"},{"id":"T7","span":{"begin":190,"end":213},"obj":"DNA"},{"id":"T8","span":{"begin":215,"end":218},"obj":"DNA"},{"id":"T9","span":{"begin":250,"end":279},"obj":"DNA"},{"id":"T10","span":{"begin":287,"end":296},"obj":"DNA"},{"id":"T11","span":{"begin":304,"end":330},"obj":"DNA"},{"id":"T12","span":{"begin":332,"end":335},"obj":"DNA"},{"id":"T13","span":{"begin":344,"end":372},"obj":"DNA"},{"id":"T14","span":{"begin":381,"end":384},"obj":"DNA"},{"id":"T15","span":{"begin":400,"end":404},"obj":"protein"},{"id":"T16","span":{"begin":409,"end":417},"obj":"protein"},{"id":"T17","span":{"begin":418,"end":439},"obj":"protein"},{"id":"T18","span":{"begin":476,"end":479},"obj":"DNA"},{"id":"T19","span":{"begin":489,"end":513},"obj":"DNA"},{"id":"T20","span":{"begin":515,"end":518},"obj":"DNA"},{"id":"T21","span":{"begin":572,"end":585},"obj":"protein"},{"id":"T22","span":{"begin":587,"end":590},"obj":"protein"},{"id":"T23","span":{"begin":634,"end":650},"obj":"protein"},{"id":"T24","span":{"begin":652,"end":655},"obj":"protein"},{"id":"T25","span":{"begin":732,"end":741},"obj":"DNA"},{"id":"T26","span":{"begin":813,"end":816},"obj":"protein"},{"id":"T27","span":{"begin":821,"end":829},"obj":"protein"},{"id":"T28","span":{"begin":831,"end":834},"obj":"protein"},{"id":"T29","span":{"begin":849,"end":857},"obj":"protein"},{"id":"T30","span":{"begin":861,"end":869},"obj":"protein"},{"id":"T31","span":{"begin":907,"end":918},"obj":"protein"},{"id":"T32","span":{"begin":933,"end":954},"obj":"cell_line"},{"id":"T33","span":{"begin":980,"end":1003},"obj":"DNA"},{"id":"T34","span":{"begin":1035,"end":1044},"obj":"DNA"},{"id":"T35","span":{"begin":1059,"end":1062},"obj":"DNA"},{"id":"T36","span":{"begin":1091,"end":1094},"obj":"protein"},{"id":"T37","span":{"begin":1099,"end":1107},"obj":"protein"},{"id":"T38","span":{"begin":1140,"end":1143},"obj":"protein"},{"id":"T39","span":{"begin":1148,"end":1156},"obj":"protein"},{"id":"T40","span":{"begin":1184,"end":1192},"obj":"protein"},{"id":"T41","span":{"begin":1197,"end":1205},"obj":"protein"},{"id":"T42","span":{"begin":1241,"end":1245},"obj":"protein"},{"id":"T43","span":{"begin":1295,"end":1303},"obj":"cell_line"},{"id":"T44","span":{"begin":1317,"end":1320},"obj":"DNA"},{"id":"T45","span":{"begin":1330,"end":1333},"obj":"DNA"},{"id":"T46","span":{"begin":1395,"end":1399},"obj":"protein"},{"id":"T47","span":{"begin":1425,"end":1433},"obj":"protein"},{"id":"T48","span":{"begin":1445,"end":1453},"obj":"protein"},{"id":"T49","span":{"begin":1470,"end":1473},"obj":"protein"},{"id":"T50","span":{"begin":1513,"end":1517},"obj":"protein"},{"id":"T51","span":{"begin":1571,"end":1605},"obj":"DNA"},{"id":"T52","span":{"begin":1617,"end":1639},"obj":"DNA"},{"id":"T53","span":{"begin":1694,"end":1702},"obj":"protein"},{"id":"T54","span":{"begin":1715,"end":1719},"obj":"protein"},{"id":"T55","span":{"begin":1739,"end":1742},"obj":"DNA"},{"id":"T56","span":{"begin":1747,"end":1750},"obj":"DNA"},{"id":"T57","span":{"begin":1769,"end":1788},"obj":"protein"},{"id":"T58","span":{"begin":1832,"end":1835},"obj":"DNA"},{"id":"T59","span":{"begin":1841,"end":1853},"obj":"protein"},{"id":"T60","span":{"begin":1871,"end":1897},"obj":"protein"}],"text":"TNFalpha cooperates with the protein kinase A pathway to synergistically increase HIV-1 LTR transcription via downstream TRE-like cAMP response elements.\nActivating protein-1 (AP-1) binding TPA responsive elements (TRE) are located downstream of the transcription initiation site in the U5 region of the HIV-1 long terminal repeat (LTR). These downstream sequence elements, termed DSE, can bind both AP-1 and CREB/ATF transcription factors. Recently, we demonstrated that the DSE are also cAMP-responsive elements (CRE), since they mediated activation signals elicited by cholera toxin (Ctx), a potent activator of the cAMP-dependent protein kinase A (PKA) signal transduction pathway. In the present study, we demonstrate that the HIV-1 DSE can mediate the transcriptional synergy elicited by the combination of Ctx and TNFalpha. Ctx combined with TNFalpha or IL-1beta to produce a synergistic increase in p24 antigen production in U1 promonocytic cells. Transfection studies of LTR reporter constructs indicated that mutation of the DSE sites abrogated the LTR-mediated synergy induced by Ctx and TNFalpha, whereas the synergy induced by Ctx and IL-1beta was unaffected, suggesting TNFalpha and IL-1beta cooperate differently with the cAMP/PKA activation pathway to induce HIV-1 expression in U1 cells. Because the DSE are also TRE sites, we assessed the effect of the agonist combinations on AP-1-dependent transcription. TNFalpha as well as IL-1beta cooperated with Ctx to produce a synergistic activation of AP-1-mediated transcription. These data indicate that the TRE-like cAMP-responsive DSE sites within the 5'-untranslated leader can mediate the transcriptional cooperativity between TNFalpha and the cAMP/PKA pathway. Since the DSE and TRE sites cannot bind CREB/ATF homodimers, we propose a mechanism in which the HIV-1 DSE bind heterodimers composed of both AP-1 and CREB/ATF proteins."}

    pubmed-sentences-benchmark

    {"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":153},"obj":"Sentence"},{"id":"S2","span":{"begin":154,"end":337},"obj":"Sentence"},{"id":"S3","span":{"begin":338,"end":440},"obj":"Sentence"},{"id":"S4","span":{"begin":441,"end":685},"obj":"Sentence"},{"id":"S5","span":{"begin":686,"end":830},"obj":"Sentence"},{"id":"S6","span":{"begin":831,"end":955},"obj":"Sentence"},{"id":"S7","span":{"begin":956,"end":1304},"obj":"Sentence"},{"id":"S8","span":{"begin":1305,"end":1424},"obj":"Sentence"},{"id":"S9","span":{"begin":1425,"end":1541},"obj":"Sentence"},{"id":"S10","span":{"begin":1542,"end":1728},"obj":"Sentence"},{"id":"S11","span":{"begin":1729,"end":1898},"obj":"Sentence"}],"text":"TNFalpha cooperates with the protein kinase A pathway to synergistically increase HIV-1 LTR transcription via downstream TRE-like cAMP response elements.\nActivating protein-1 (AP-1) binding TPA responsive elements (TRE) are located downstream of the transcription initiation site in the U5 region of the HIV-1 long terminal repeat (LTR). These downstream sequence elements, termed DSE, can bind both AP-1 and CREB/ATF transcription factors. Recently, we demonstrated that the DSE are also cAMP-responsive elements (CRE), since they mediated activation signals elicited by cholera toxin (Ctx), a potent activator of the cAMP-dependent protein kinase A (PKA) signal transduction pathway. In the present study, we demonstrate that the HIV-1 DSE can mediate the transcriptional synergy elicited by the combination of Ctx and TNFalpha. Ctx combined with TNFalpha or IL-1beta to produce a synergistic increase in p24 antigen production in U1 promonocytic cells. Transfection studies of LTR reporter constructs indicated that mutation of the DSE sites abrogated the LTR-mediated synergy induced by Ctx and TNFalpha, whereas the synergy induced by Ctx and IL-1beta was unaffected, suggesting TNFalpha and IL-1beta cooperate differently with the cAMP/PKA activation pathway to induce HIV-1 expression in U1 cells. Because the DSE are also TRE sites, we assessed the effect of the agonist combinations on AP-1-dependent transcription. TNFalpha as well as IL-1beta cooperated with Ctx to produce a synergistic activation of AP-1-mediated transcription. These data indicate that the TRE-like cAMP-responsive DSE sites within the 5'-untranslated leader can mediate the transcriptional cooperativity between TNFalpha and the cAMP/PKA pathway. Since the DSE and TRE sites cannot bind CREB/ATF homodimers, we propose a mechanism in which the HIV-1 DSE bind heterodimers composed of both AP-1 and CREB/ATF proteins."}

    genia-medco-coref

    {"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":0,"end":8},"obj":"NP"},{"id":"C2","span":{"begin":154,"end":219},"obj":"NP"},{"id":"C3","span":{"begin":338,"end":372},"obj":"NP"},{"id":"C4","span":{"begin":381,"end":384},"obj":"NP"},{"id":"C5","span":{"begin":472,"end":479},"obj":"NP"},{"id":"C6","span":{"begin":527,"end":531},"obj":"NP"},{"id":"C7","span":{"begin":572,"end":591},"obj":"NP"},{"id":"C8","span":{"begin":593,"end":684},"obj":"NP"},{"id":"C9","span":{"begin":728,"end":741},"obj":"NP"},{"id":"C11","span":{"begin":813,"end":816},"obj":"NP"},{"id":"C12","span":{"begin":821,"end":829},"obj":"NP"},{"id":"C10","span":{"begin":813,"end":829},"obj":"NP"},{"id":"C13","span":{"begin":831,"end":834},"obj":"NP"},{"id":"C14","span":{"begin":849,"end":857},"obj":"NP"},{"id":"C15","span":{"begin":861,"end":869},"obj":"NP"},{"id":"C16","span":{"begin":933,"end":954},"obj":"NP"},{"id":"C18","span":{"begin":1091,"end":1094},"obj":"NP"},{"id":"C19","span":{"begin":1099,"end":1107},"obj":"NP"},{"id":"C17","span":{"begin":1091,"end":1107},"obj":"NP"},{"id":"C20","span":{"begin":1140,"end":1143},"obj":"NP"},{"id":"C21","span":{"begin":1148,"end":1156},"obj":"NP"},{"id":"C23","span":{"begin":1184,"end":1192},"obj":"NP"},{"id":"C24","span":{"begin":1197,"end":1205},"obj":"NP"},{"id":"C22","span":{"begin":1184,"end":1205},"obj":"NP"},{"id":"C25","span":{"begin":1295,"end":1303},"obj":"NP"},{"id":"C26","span":{"begin":1313,"end":1320},"obj":"NP"},{"id":"C27","span":{"begin":1330,"end":1339},"obj":"NP"},{"id":"C29","span":{"begin":1425,"end":1433},"obj":"NP"},{"id":"C30","span":{"begin":1445,"end":1453},"obj":"NP"},{"id":"C28","span":{"begin":1425,"end":1453},"obj":"NP"},{"id":"C31","span":{"begin":1470,"end":1473},"obj":"NP"},{"id":"C32","span":{"begin":1694,"end":1702},"obj":"NP"},{"id":"C33","span":{"begin":1735,"end":1742},"obj":"NP"},{"id":"C34","span":{"begin":1747,"end":1756},"obj":"NP"},{"id":"C35","span":{"begin":1801,"end":1812},"obj":"NP"},{"id":"C36","span":{"begin":1816,"end":1821},"obj":"NP"},{"id":"C37","span":{"begin":1822,"end":1835},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C3","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C4","obj":"C3"},{"id":"R3","pred":"coref-pron","subj":"C6","obj":"C5"},{"id":"R4","pred":"coref-appos","subj":"C8","obj":"C7"},{"id":"R5","pred":"coref-ident","subj":"C11","obj":"C7"},{"id":"R6","pred":"coref-ident","subj":"C12","obj":"C1"},{"id":"R7","pred":"coref-ident","subj":"C14","obj":"C12"},{"id":"R8","pred":"coref-ident","subj":"C18","obj":"C13"},{"id":"R9","pred":"coref-ident","subj":"C19","obj":"C14"},{"id":"R10","pred":"coref-ident","subj":"C17","obj":"C10"},{"id":"R11","pred":"coref-ident","subj":"C20","obj":"C18"},{"id":"R12","pred":"coref-ident","subj":"C21","obj":"C15"},{"id":"R13","pred":"coref-ident","subj":"C23","obj":"C19"},{"id":"R14","pred":"coref-ident","subj":"C24","obj":"C21"},{"id":"R15","pred":"coref-ident","subj":"C25","obj":"C16"},{"id":"R16","pred":"coref-ident","subj":"C26","obj":"C5"},{"id":"R17","pred":"coref-ident","subj":"C29","obj":"C23"},{"id":"R18","pred":"coref-ident","subj":"C30","obj":"C24"},{"id":"R19","pred":"coref-ident","subj":"C28","obj":"C22"},{"id":"R20","pred":"coref-ident","subj":"C31","obj":"C20"},{"id":"R21","pred":"coref-ident","subj":"C32","obj":"C29"},{"id":"R22","pred":"coref-ident","subj":"C33","obj":"C26"},{"id":"R23","pred":"coref-ident","subj":"C34","obj":"C27"},{"id":"R24","pred":"coref-relat","subj":"C36","obj":"C35"},{"id":"R25","pred":"coref-ident","subj":"C37","obj":"C9"}],"text":"TNFalpha cooperates with the protein kinase A pathway to synergistically increase HIV-1 LTR transcription via downstream TRE-like cAMP response elements.\nActivating protein-1 (AP-1) binding TPA responsive elements (TRE) are located downstream of the transcription initiation site in the U5 region of the HIV-1 long terminal repeat (LTR). These downstream sequence elements, termed DSE, can bind both AP-1 and CREB/ATF transcription factors. Recently, we demonstrated that the DSE are also cAMP-responsive elements (CRE), since they mediated activation signals elicited by cholera toxin (Ctx), a potent activator of the cAMP-dependent protein kinase A (PKA) signal transduction pathway. In the present study, we demonstrate that the HIV-1 DSE can mediate the transcriptional synergy elicited by the combination of Ctx and TNFalpha. Ctx combined with TNFalpha or IL-1beta to produce a synergistic increase in p24 antigen production in U1 promonocytic cells. Transfection studies of LTR reporter constructs indicated that mutation of the DSE sites abrogated the LTR-mediated synergy induced by Ctx and TNFalpha, whereas the synergy induced by Ctx and IL-1beta was unaffected, suggesting TNFalpha and IL-1beta cooperate differently with the cAMP/PKA activation pathway to induce HIV-1 expression in U1 cells. Because the DSE are also TRE sites, we assessed the effect of the agonist combinations on AP-1-dependent transcription. TNFalpha as well as IL-1beta cooperated with Ctx to produce a synergistic activation of AP-1-mediated transcription. These data indicate that the TRE-like cAMP-responsive DSE sites within the 5'-untranslated leader can mediate the transcriptional cooperativity between TNFalpha and the cAMP/PKA pathway. Since the DSE and TRE sites cannot bind CREB/ATF homodimers, we propose a mechanism in which the HIV-1 DSE bind heterodimers composed of both AP-1 and CREB/ATF proteins."}

    GENIAcorpus

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These downstream sequence elements, termed DSE, can bind both AP-1 and CREB/ATF transcription factors. Recently, we demonstrated that the DSE are also cAMP-responsive elements (CRE), since they mediated activation signals elicited by cholera toxin (Ctx), a potent activator of the cAMP-dependent protein kinase A (PKA) signal transduction pathway. In the present study, we demonstrate that the HIV-1 DSE can mediate the transcriptional synergy elicited by the combination of Ctx and TNFalpha. Ctx combined with TNFalpha or IL-1beta to produce a synergistic increase in p24 antigen production in U1 promonocytic cells. Transfection studies of LTR reporter constructs indicated that mutation of the DSE sites abrogated the LTR-mediated synergy induced by Ctx and TNFalpha, whereas the synergy induced by Ctx and IL-1beta was unaffected, suggesting TNFalpha and IL-1beta cooperate differently with the cAMP/PKA activation pathway to induce HIV-1 expression in U1 cells. Because the DSE are also TRE sites, we assessed the effect of the agonist combinations on AP-1-dependent transcription. TNFalpha as well as IL-1beta cooperated with Ctx to produce a synergistic activation of AP-1-mediated transcription. These data indicate that the TRE-like cAMP-responsive DSE sites within the 5'-untranslated leader can mediate the transcriptional cooperativity between TNFalpha and the cAMP/PKA pathway. Since the DSE and TRE sites cannot bind CREB/ATF homodimers, we propose a mechanism in which the HIV-1 DSE bind heterodimers composed of both AP-1 and CREB/ATF proteins."}