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PubMed:9286159 JSONTXT

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GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 1268-1275 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 1037-1041 Phenotype denotes A Ki HP:0001919

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 1268-1275 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-46 Sentence denotes Microchip device for performing enzyme assays.
T2 47-128 Sentence denotes An automated enzyme assay was performed within a microfabricated channel network.
T3 129-246 Sentence denotes Precise concentrations of substrate, enzyme, and inhibitor were mixed in nanoliter volumes using electrokinetic flow.
T4 247-433 Sentence denotes Reagent dilution and mixing were controlled by regulating the applied potential at the terminus of each channel, using voltages derived from an equivalent circuit model of the microchip.
T5 434-602 Sentence denotes The enzyme beta-galactosidase (beta-Gal) was assayed using resorufin beta-D-galactopyranoside (RBG), a substrate that is hydrolyzed to resorufin, a fluorescent product.
T6 603-761 Sentence denotes Reaction kinetics were obtained by varying the concentration of substrate on-chip and monitoring the production of resorufin using laser-induced fluorescence.
T7 762-863 Sentence denotes Derived Michaelis--Menten constants compared well between an on-chip and a conventional enzyme assay.
T8 864-1036 Sentence denotes Bias in the derived K(m) and kcat was primarily due to the limited solubility of RBG and the associated lack of measurements at substrate concentrations exceeding the K(m).
T9 1037-1228 Sentence denotes A Ki of 8 microM for the inhibitor phenylethyl beta-D-thiogalactoside (PETG) was determined from plots of initial rate versus substrate concentration obtained at three concentrations of PETG.
T10 1229-1419 Sentence denotes The relative inhibition of beta-Gal by lactose, p-hydroxymercuribenzoic acid, and PETG was determined by varying the inhibitor concentration with constant enzyme and substrate concentration.
T11 1420-1633 Sentence denotes An enzyme assay performed on the microchip within a 20-min period required only 120 pg of enzyme and 7.5 ng of substrate, reducing the amount of reagent consumed by 4 orders of magnitude over a conventional assay.