PubMed:9266697 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/9266697","sourcedb":"PubMed","sourceid":"9266697","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/9266697","text":"Cloning and expression of cDNA for a human Gal(beta1-3)GalNAc alpha2,3-sialyltransferase from the CEM T-cell line.\nComplementary DNA encoding a human Gal(beta1-3)GalNAc alpha2,3-sialyltransferase type II (hST3Gal II) was cloned from a CEM T-cell cDNA library using a 23-base oligonucleotide probe. The sequence of this probe was established on the basis of a slightly divergent sialylmotif L that was obtained by polymerase chain reaction with degenerate oligonucleotide primers based on the conserved sialylmotif L of mammalian Gal(beta1-3)GalNAc alpha2,3-sialyltransferases. It was thus confirmed that a short oligonucleotide probe may be sensitive and highly specific. The nucleotide and amino acid sequences of hST3Gal II show, respectively, 56.3% and 49.3% similarity to hST3Gal I [Kitagawa, H. \u0026 Paulson, J. C. (1994) J. Biol. Chem. 269, 17872-17878] and 88.1% and 93.7% similarity to murine ST3Gal II [Lee, Y. C., Kojima, N., Wada, E., Kurosawa, N., Nakaoka, T., Hamamoto, T. \u0026 Tsuji, S. (1994) J. Biol. Chem. 269, 10028-10033]. hST3Gal II mRNA was highly expressed in heart, liver, skeletal muscle and various lymphoid tissues but not in brain and kidney. A soluble form of hST3Gal II expressed in COS-7 cells was tested in vitro for substrate specificity and kinetic properties. Asialofetuin and asialo-bovine submaxillary mucin appeared better substrates for hST3Gal II than for its murine counterpart as previously reported [Kojima, N., Lee, Y.-C., Hamamoto, T., Kurosawa, N. \u0026 Tsuji, S. (1994) Biochemistry 33, 5772-5776]. In previous studies, we have shown hyposialylation of O-glycans attached to two major lymphocyte CD43 and CD45 cell surface molecules in human-immunodeficiency-virus-1(HIV-1)-infected T-cell lines. Since comparable levels of hST3Gal I and hST3Gal II mRNA and enzymatic activity were observed in parental and HIV-1-infected CEM T-cell lysates, the sialylation defect associated with HIV infection of this cell line is probably due to a mechanism different from a simple altered catalytic activity of these sialyltransferases.","tracks":[{"project":"ggdb-test","denotations":[{"id":"T1","span":{"begin":898,"end":907},"obj":"https://acgg.asia/db/ggdb/info/gg050"},{"id":"T2","span":{"begin":898,"end":907},"obj":"https://acgg.asia/db/ggdb/info/gg049"}],"attributes":[{"subj":"T1","pred":"source","obj":"ggdb-test"},{"subj":"T2","pred":"source","obj":"ggdb-test"}]},{"project":"GGDB-2020","denotations":[{"id":"T1","span":{"begin":898,"end":907},"obj":"https://acgg.asia/db/ggdb/info/gg049"},{"id":"T2","span":{"begin":898,"end":907},"obj":"https://acgg.asia/db/ggdb/info/gg050"}],"attributes":[{"subj":"T1","pred":"source","obj":"GGDB-2020"},{"subj":"T2","pred":"source","obj":"GGDB-2020"}]},{"project":"glycogenes","denotations":[{"id":"PD-GlycoGenes20190927-B_T1","span":{"begin":201,"end":203},"obj":"https://acgg.asia/db/ggdb/info/gg111"},{"id":"PD-GlycoGenes20190927-B_T2","span":{"begin":213,"end":215},"obj":"https://acgg.asia/db/ggdb/info/gg111"},{"id":"PD-GlycoGenes20190927-B_T3","span":{"begin":723,"end":725},"obj":"https://acgg.asia/db/ggdb/info/gg111"},{"id":"PD-GlycoGenes20190927-B_T4","span":{"begin":898,"end":907},"obj":"https://acgg.asia/db/ggdb/info/gg050"},{"id":"PD-GlycoGenes20190927-B_T5","span":{"begin":898,"end":907},"obj":"https://acgg.asia/db/ggdb/info/gg049"},{"id":"PD-GlycoGenes20190927-B_T6","span":{"begin":1044,"end":1046},"obj":"https://acgg.asia/db/ggdb/info/gg111"},{"id":"PD-GlycoGenes20190927-B_T7","span":{"begin":1190,"end":1192},"obj":"https://acgg.asia/db/ggdb/info/gg111"},{"id":"PD-GlycoGenes20190927-B_T8","span":{"begin":1377,"end":1379},"obj":"https://acgg.asia/db/ggdb/info/gg111"},{"id":"PD-GlycoGenes20190927-B_T9","span":{"begin":1782,"end":1784},"obj":"https://acgg.asia/db/ggdb/info/gg111"}],"attributes":[{"subj":"PD-GlycoGenes20190927-B_T1","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T2","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T3","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T4","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T5","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T6","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T7","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T8","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T9","pred":"source","obj":"glycogenes"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"ggdb-test","color":"#ec93a5","default":true},{"id":"GGDB-2020","color":"#93bfec"},{"id":"glycogenes","color":"#d9ec93"}]}]}}