> top > docs > PubMed:9153220 > annotations

PubMed:9153220 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
T1 0-57 Sentence denotes Characterization of functional domains within Smad4/DPC4.
T2 58-258 Sentence denotes Smad proteins are a family of highly conserved, intracellular proteins that signal cellular responses downstream of transforming growth factor-beta (TGF-beta) family serine/threonine kinase receptors.
T3 259-601 Sentence denotes One of these molecules, Smad4, originally identified as the candidate tumor suppressor gene dpc-4, reconstitutes TGF-beta- and activin-dependent transcriptional responses in Smad4 null cell lines and interacts in a ligand-dependent manner with other Smad family members in both TGF-beta, activin, and bone morphogenetic protein-2/-4 pathways.
T4 602-780 Sentence denotes Here, we used an assay based on the restoration of ligand-dependent transcriptional responses in a Smad4 null cell line to characterize functional domain structures within Smad4.
T5 781-1086 Sentence denotes We showed that restoration of TGF-beta-induced transcriptional responses by Smad4 was inhibited by co-transfection with a kinase dead TGF-beta type II receptor and that constitutive activation was blocked with TGF-beta neutralizing antibodies, confirming the essential role of Smad4 in TGF-beta signaling.
T6 1087-1305 Sentence denotes Using a series of Smad4 mutation, deletion, and Smad1/Smad4 chimera constructs we identified a 47-amino acid deletion within the middle-linker region of Smad4 that is essential for the mediation of signaling responses.
T7 1306-1544 Sentence denotes In addition, we showed that the NH2-terminal domain of Smad4 augments ligand-dependent activation associated with the middle-linker region, indicating that there is a distinct ligand-response domain within the N terminus of this molecule.
T1 0-57 Sentence denotes Characterization of functional domains within Smad4/DPC4.
T2 58-258 Sentence denotes Smad proteins are a family of highly conserved, intracellular proteins that signal cellular responses downstream of transforming growth factor-beta (TGF-beta) family serine/threonine kinase receptors.
T3 259-601 Sentence denotes One of these molecules, Smad4, originally identified as the candidate tumor suppressor gene dpc-4, reconstitutes TGF-beta- and activin-dependent transcriptional responses in Smad4 null cell lines and interacts in a ligand-dependent manner with other Smad family members in both TGF-beta, activin, and bone morphogenetic protein-2/-4 pathways.
T4 602-780 Sentence denotes Here, we used an assay based on the restoration of ligand-dependent transcriptional responses in a Smad4 null cell line to characterize functional domain structures within Smad4.
T5 781-1086 Sentence denotes We showed that restoration of TGF-beta-induced transcriptional responses by Smad4 was inhibited by co-transfection with a kinase dead TGF-beta type II receptor and that constitutive activation was blocked with TGF-beta neutralizing antibodies, confirming the essential role of Smad4 in TGF-beta signaling.
T6 1087-1305 Sentence denotes Using a series of Smad4 mutation, deletion, and Smad1/Smad4 chimera constructs we identified a 47-amino acid deletion within the middle-linker region of Smad4 that is essential for the mediation of signaling responses.
T7 1306-1544 Sentence denotes In addition, we showed that the NH2-terminal domain of Smad4 augments ligand-dependent activation associated with the middle-linker region, indicating that there is a distinct ligand-response domain within the N terminus of this molecule.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 560-564 http://purl.obolibrary.org/obo/MAT_0000299 denotes bone

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 329-334 Phenotype denotes tumor HP:0002664

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 329-334 Disease denotes tumor http://purl.obolibrary.org/obo/MONDO_0005070

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 106-119 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T2 560-564 Body_part denotes bone http://purl.obolibrary.org/obo/UBERON_0001474|http://purl.obolibrary.org/obo/UBERON_0002481

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 560-564 Body_part denotes bone http://purl.obolibrary.org/obo/MAT_0000299

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 439-448 Cell denotes null cell http://purl.obolibrary.org/obo/CL:0000623|http://purl.obolibrary.org/obo/CL:0000825
T3 707-716 Cell denotes null cell http://purl.obolibrary.org/obo/CL:0000623|http://purl.obolibrary.org/obo/CL:0000825