PubMed:9112333 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/9112333","sourcedb":"PubMed","sourceid":"9112333","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/9112333","text":"Interleukin 1 receptor antagonist localization exclusively in non-parenchymal cells of the mouse liver.\nThe natural interleukin 1 receptor antagonist (IL-1ra) is induced in mouse liver after lipopolysaccharide (LPS) or recombinant interleukin 1 (IL-1) administration. Changes in hepatic IL-1ra mRNA concentration were measured following LPS injection to CBA mice. The results showed, that in vivo, IL-1ra transcripts were stimulated early at about 2h with maximal elevation at 4-6 h. Northern blot analysis of RNA extracted from primary cultures of mouse hepatocytes did not detect IL-1ra mRNA, although constitutive and stimulated contrapsin mRNA expression were easily demonstrated. Therefore, induction of IL-1ra mRNA in sinusoid lining cells of the mouse liver at 4 h after LPS injection was evidenced by in situ hybridization using the same specific cDNA probe as in the Northern blotting technique. Additionally, immunohistochemical studies revealed that IL-1 receptor antagonist protein was induced with LPS after 19 hours in sinusoid lining cells. The data deriving from Northern blotting, in situ hybridization and immunohistochemical studies, suggest that IL-1ra is formed exclusively in non-parenchymal liver cells.","tracks":[{"project":"2015-BEL-Sample","denotations":[{"id":"T1","span":{"begin":116,"end":244},"obj":"a(CHEBI:lipopolysaccharide) increases p(MGI:Il1rn)"}],"attributes":[{"subj":"T1","pred":"source","obj":"2015-BEL-Sample"}]},{"project":"2015-BEL-Sample-2","denotations":[{"id":"BEL:20000030","span":{"begin":116,"end":244},"obj":"a(CHEBI:lipopolysaccharide) increases p(MGI:Il1rn)"},{"id":"BEL:20000030","span":{"begin":116,"end":244},"obj":"a(CHEBI:lipopolysaccharide) increases p(MGI:Il1rn)"},{"id":"BEL:20024440","span":{"begin":116,"end":249},"obj":"a(CHEBI:lipopolysaccharide) increases p(MGI:Il1rn)"},{"id":"BEL:20040240","span":{"begin":116,"end":249},"obj":"p(MGI:Il1a) increases p(MGI:Il1rn)"}],"attributes":[{"subj":"BEL:20000030","pred":"source","obj":"2015-BEL-Sample-2"},{"subj":"BEL:20000030","pred":"source","obj":"2015-BEL-Sample-2"},{"subj":"BEL:20024440","pred":"source","obj":"2015-BEL-Sample-2"},{"subj":"BEL:20040240","pred":"source","obj":"2015-BEL-Sample-2"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2015-BEL-Sample","color":"#93cfec","default":true},{"id":"2015-BEL-Sample-2","color":"#eaec93"}]}]}}