| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-150 |
Sentence |
denotes |
Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. |
| T2 |
151-337 |
Sentence |
denotes |
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). |
| T3 |
338-462 |
Sentence |
denotes |
MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. |
| T4 |
463-587 |
Sentence |
denotes |
We report herein the isolation of a cDNA encoding a novel protein kinase designated MAPKKK5 from a human macrophage library. |
| T5 |
588-714 |
Sentence |
denotes |
The nucleotide sequence predicts that MAPKKK5 encodes an open reading frame of 1374 amino acids with all 11 kinase subdomains. |
| T6 |
715-978 |
Sentence |
denotes |
The putative catalytic domain of MAPKKK5 shows significant sequence homology to the kinase domains of the MAPKKK/MEKK level protein kinases from mouse MEKK2 and -3, Drosophila melanogaster PK92B, Saccharomyces cerevisiae STE11, and Schizosaccharomyces pombe BYR2. |
| T7 |
979-1085 |
Sentence |
denotes |
Northern blot analysis showed that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. |
| T8 |
1086-1239 |
Sentence |
denotes |
When transiently expressed in COS and 293 cells, MAPKKK5 markedly activated c-Jun N-terminal kinase or stress-activated protein kinase, but not MAPK/ERK. |
| T9 |
1240-1470 |
Sentence |
denotes |
Furthermore, MAPKKK5 that was immunoprecipitated from transfected 293 cells was able to phosphorylate and activate MKK4 in vitro, suggesting that MAPKKK5 may be an upstream activator of MKK4 in the c-Jun N-terminal kinase pathway. |
| T1 |
0-150 |
Sentence |
denotes |
Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. |
| T2 |
151-337 |
Sentence |
denotes |
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). |
| T3 |
338-462 |
Sentence |
denotes |
MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. |
| T4 |
463-587 |
Sentence |
denotes |
We report herein the isolation of a cDNA encoding a novel protein kinase designated MAPKKK5 from a human macrophage library. |
| T5 |
588-714 |
Sentence |
denotes |
The nucleotide sequence predicts that MAPKKK5 encodes an open reading frame of 1374 amino acids with all 11 kinase subdomains. |
| T6 |
715-978 |
Sentence |
denotes |
The putative catalytic domain of MAPKKK5 shows significant sequence homology to the kinase domains of the MAPKKK/MEKK level protein kinases from mouse MEKK2 and -3, Drosophila melanogaster PK92B, Saccharomyces cerevisiae STE11, and Schizosaccharomyces pombe BYR2. |
| T7 |
979-1085 |
Sentence |
denotes |
Northern blot analysis showed that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. |
| T8 |
1086-1239 |
Sentence |
denotes |
When transiently expressed in COS and 293 cells, MAPKKK5 markedly activated c-Jun N-terminal kinase or stress-activated protein kinase, but not MAPK/ERK. |
| T9 |
1240-1470 |
Sentence |
denotes |
Furthermore, MAPKKK5 that was immunoprecipitated from transfected 293 cells was able to phosphorylate and activate MKK4 in vitro, suggesting that MAPKKK5 may be an upstream activator of MKK4 in the c-Jun N-terminal kinase pathway. |