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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 388-394 Glycan_Motif denotes core 2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00033MO

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-77 Sentence denotes An approach for fluorometric determination of glycosyltransferase activities.
TextSentencer_T2 78-174 Sentence denotes A new strategy for the fluorometric determination of glycosyltransferase activities is reported.
TextSentencer_T3 175-358 Sentence denotes The method involves dansyl chloride derivatization of the reduced form (pNH2phenyl) of a hydrophobic, aglycon moiety covalently linked to a number of acceptor substrates (pNO2phenyl).
TextSentencer_T4 359-543 Sentence denotes Focusing on the Golgi enzyme core 2 N-acetyl-glucosaminyltransferase, we found that synthesis and fractionation of the dansylated substrate derivative were rapid, easy and inexpensive.
TextSentencer_T5 544-683 Sentence denotes Additionally, the corresponding enzyme assay proved reproducible and very sensitive, as 0.4 pmol of reaction product were readily detected.
TextSentencer_T6 684-870 Sentence denotes This fluorometric approach appears therefore to be a valid tool for investigating the monitoring differential expression of glycosyltransferases exhibiting low levels of enzyme activity.
T1 0-77 Sentence denotes An approach for fluorometric determination of glycosyltransferase activities.
T2 78-174 Sentence denotes A new strategy for the fluorometric determination of glycosyltransferase activities is reported.
T3 175-358 Sentence denotes The method involves dansyl chloride derivatization of the reduced form (pNH2phenyl) of a hydrophobic, aglycon moiety covalently linked to a number of acceptor substrates (pNO2phenyl).
T4 359-543 Sentence denotes Focusing on the Golgi enzyme core 2 N-acetyl-glucosaminyltransferase, we found that synthesis and fractionation of the dansylated substrate derivative were rapid, easy and inexpensive.
T5 544-683 Sentence denotes Additionally, the corresponding enzyme assay proved reproducible and very sensitive, as 0.4 pmol of reaction product were readily detected.
T6 684-870 Sentence denotes This fluorometric approach appears therefore to be a valid tool for investigating the monitoring differential expression of glycosyltransferases exhibiting low levels of enzyme activity.

GlyCosmos600-GlycoProteins

Id Subject Object Predicate Lexical cue
PD-GlycoProteins-B_T1 395-427 http://purl.uniprot.org/uniprot/Q8N0V5 denotes N-acetyl-glucosaminyltransferase

pubmed-enju-pas

Id Subject Object Predicate Lexical cue
EnjuParser_T0 0-2 DT denotes An
EnjuParser_T1 3-11 NN denotes approach
EnjuParser_T2 12-15 IN denotes for
EnjuParser_T3 16-28 JJ denotes fluorometric
EnjuParser_T4 29-42 NN denotes determination
EnjuParser_T5 43-45 IN denotes of
EnjuParser_T6 46-65 NN denotes glycosyltransferase
EnjuParser_T7 66-76 NNS denotes activities
EnjuParser_T8 78-79 DT denotes A
EnjuParser_T9 80-83 JJ denotes new
EnjuParser_T10 84-92 NN denotes strategy
EnjuParser_T11 93-96 IN denotes for
EnjuParser_T12 97-100 DT denotes the
EnjuParser_T13 101-113 JJ denotes fluorometric
EnjuParser_T14 114-127 NN denotes determination
EnjuParser_T15 128-130 IN denotes of
EnjuParser_T16 131-150 NN denotes glycosyltransferase
EnjuParser_T17 151-161 NNS denotes activities
EnjuParser_T18 162-164 VBZ denotes is
EnjuParser_T19 165-173 VBN denotes reported
EnjuParser_T20 175-178 DT denotes The
EnjuParser_T21 179-185 NN denotes method
EnjuParser_T22 186-194 VBZ denotes involves
EnjuParser_T23 195-201 NN denotes dansyl
EnjuParser_T24 202-210 NN denotes chloride
EnjuParser_T25 211-225 NN denotes derivatization
EnjuParser_T26 226-228 IN denotes of
EnjuParser_T27 229-232 DT denotes the
EnjuParser_T28 233-240 VBN denotes reduced
EnjuParser_T29 241-245 NN denotes form
EnjuParser_T30 246-247 -LRB- denotes (
EnjuParser_T31 247-257 NN denotes pNH2phenyl
EnjuParser_T32 257-258 -RRB- denotes )
EnjuParser_T33 259-261 IN denotes of
EnjuParser_T34 262-263 DT denotes a
EnjuParser_T35 264-275 JJ denotes hydrophobic
EnjuParser_T36 275-276 -COMMA- denotes ,
EnjuParser_T37 277-284 NN denotes aglycon
EnjuParser_T38 285-291 NN denotes moiety
EnjuParser_T39 292-302 RB denotes covalently
EnjuParser_T40 303-309 VBN denotes linked
EnjuParser_T41 310-312 TO denotes to
EnjuParser_T42 313-314 DT denotes a
EnjuParser_T43 315-321 NN denotes number
EnjuParser_T44 322-324 IN denotes of
EnjuParser_T45 325-333 NN denotes acceptor
EnjuParser_T46 334-344 NNS denotes substrates
EnjuParser_T47 345-346 -LRB- denotes (
EnjuParser_T48 346-356 NN denotes pNO2phenyl
EnjuParser_T49 356-357 -RRB- denotes )
EnjuParser_T50 359-367 VBG denotes Focusing
EnjuParser_T51 368-370 IN denotes on
EnjuParser_T52 371-374 DT denotes the
EnjuParser_T53 375-380 NNP denotes Golgi
EnjuParser_T54 381-387 NN denotes enzyme
EnjuParser_T55 388-392 NN denotes core
EnjuParser_T56 393-394 CD denotes 2
EnjuParser_T57 395-427 NN denotes N-acetyl-glucosaminyltransferase
EnjuParser_T58 427-428 -COMMA- denotes ,
EnjuParser_T59 429-431 PRP denotes we
EnjuParser_T60 432-437 VBD denotes found
EnjuParser_T61 438-442 IN denotes that
EnjuParser_T62 443-452 NN denotes synthesis
EnjuParser_T63 453-456 CC denotes and
EnjuParser_T64 457-470 NN denotes fractionation
EnjuParser_T65 471-473 IN denotes of
EnjuParser_T66 474-477 DT denotes the
EnjuParser_T67 478-488 VBN denotes dansylated
EnjuParser_T68 489-498 NN denotes substrate
EnjuParser_T69 499-509 NN denotes derivative
EnjuParser_T70 510-514 VBD denotes were
EnjuParser_T71 515-520 JJ denotes rapid
EnjuParser_T72 520-521 -COMMA- denotes ,
EnjuParser_T73 522-526 JJ denotes easy
EnjuParser_T74 527-530 CC denotes and
EnjuParser_T75 531-542 JJ denotes inexpensive
EnjuParser_T76 544-556 RB denotes Additionally
EnjuParser_T77 556-557 -COMMA- denotes ,
EnjuParser_T78 558-561 DT denotes the
EnjuParser_T79 562-575 VBG denotes corresponding
EnjuParser_T80 576-582 NN denotes enzyme
EnjuParser_T81 583-588 NN denotes assay
EnjuParser_T82 589-595 VBD denotes proved
EnjuParser_T83 596-608 JJ denotes reproducible
EnjuParser_T84 609-612 CC denotes and
EnjuParser_T85 613-617 RB denotes very
EnjuParser_T86 618-627 JJ denotes sensitive
EnjuParser_T87 627-628 -COMMA- denotes ,
EnjuParser_T88 629-631 IN denotes as
EnjuParser_T89 632-635 CD denotes 0.4
EnjuParser_T90 636-640 NN denotes pmol
EnjuParser_T91 641-643 IN denotes of
EnjuParser_T92 644-652 NN denotes reaction
EnjuParser_T93 653-660 NN denotes product
EnjuParser_T94 661-665 VBD denotes were
EnjuParser_T95 666-673 RB denotes readily
EnjuParser_T96 674-682 VBN denotes detected
EnjuParser_T97 684-688 DT denotes This
EnjuParser_T98 689-701 JJ denotes fluorometric
EnjuParser_T99 702-710 NN denotes approach
EnjuParser_T100 711-718 VBZ denotes appears
EnjuParser_T101 719-728 RB denotes therefore
EnjuParser_T102 729-731 TO denotes to
EnjuParser_T103 732-734 VB denotes be
EnjuParser_T104 735-736 DT denotes a
EnjuParser_T105 737-742 JJ denotes valid
EnjuParser_T106 743-747 NN denotes tool
EnjuParser_T107 748-751 IN denotes for
EnjuParser_T108 752-765 VBG denotes investigating
EnjuParser_T109 766-769 DT denotes the
EnjuParser_T110 770-780 VBG denotes monitoring
EnjuParser_T111 781-793 JJ denotes differential
EnjuParser_T112 794-804 NN denotes expression
EnjuParser_T113 805-807 IN denotes of
EnjuParser_T114 808-828 NNS denotes glycosyltransferases
EnjuParser_T115 829-839 VBG denotes exhibiting
EnjuParser_T116 840-843 JJ denotes low
EnjuParser_T117 844-850 NNS denotes levels
EnjuParser_T118 851-853 IN denotes of
EnjuParser_T119 854-860 NN denotes enzyme
EnjuParser_T120 861-869 NN denotes activity
EnjuParser_R0 EnjuParser_T1 EnjuParser_T0 arg1Of approach,An
EnjuParser_R1 EnjuParser_T1 EnjuParser_T2 arg1Of approach,for
EnjuParser_R2 EnjuParser_T4 EnjuParser_T2 arg2Of determination,for
EnjuParser_R3 EnjuParser_T4 EnjuParser_T3 arg1Of determination,fluorometric
EnjuParser_R4 EnjuParser_T4 EnjuParser_T5 arg1Of determination,of
EnjuParser_R5 EnjuParser_T7 EnjuParser_T5 arg2Of activities,of
EnjuParser_R6 EnjuParser_T7 EnjuParser_T6 arg1Of activities,glycosyltransferase
EnjuParser_R7 EnjuParser_T10 EnjuParser_T8 arg1Of strategy,A
EnjuParser_R8 EnjuParser_T10 EnjuParser_T9 arg1Of strategy,new
EnjuParser_R9 EnjuParser_T10 EnjuParser_T11 arg1Of strategy,for
EnjuParser_R10 EnjuParser_T14 EnjuParser_T11 arg2Of determination,for
EnjuParser_R11 EnjuParser_T14 EnjuParser_T12 arg1Of determination,the
EnjuParser_R12 EnjuParser_T14 EnjuParser_T13 arg1Of determination,fluorometric
EnjuParser_R13 EnjuParser_T14 EnjuParser_T15 arg1Of determination,of
EnjuParser_R14 EnjuParser_T17 EnjuParser_T15 arg2Of activities,of
EnjuParser_R15 EnjuParser_T17 EnjuParser_T16 arg1Of activities,glycosyltransferase
EnjuParser_R16 EnjuParser_T10 EnjuParser_T18 arg1Of strategy,is
EnjuParser_R17 EnjuParser_T19 EnjuParser_T18 arg2Of reported,is
EnjuParser_R18 EnjuParser_T10 EnjuParser_T19 arg2Of strategy,reported
EnjuParser_R19 EnjuParser_T21 EnjuParser_T20 arg1Of method,The
EnjuParser_R20 EnjuParser_T21 EnjuParser_T22 arg1Of method,involves
EnjuParser_R21 EnjuParser_T25 EnjuParser_T22 arg2Of derivatization,involves
EnjuParser_R22 EnjuParser_T25 EnjuParser_T23 arg1Of derivatization,dansyl
EnjuParser_R23 EnjuParser_T25 EnjuParser_T24 arg1Of derivatization,chloride
EnjuParser_R24 EnjuParser_T25 EnjuParser_T26 arg1Of derivatization,of
EnjuParser_R25 EnjuParser_T29 EnjuParser_T26 arg2Of form,of
EnjuParser_R26 EnjuParser_T29 EnjuParser_T27 arg1Of form,the
EnjuParser_R27 EnjuParser_T29 EnjuParser_T28 arg2Of form,reduced
EnjuParser_R28 EnjuParser_T29 EnjuParser_T30 arg1Of form,(
EnjuParser_R29 EnjuParser_T31 EnjuParser_T30 arg2Of pNH2phenyl,(
EnjuParser_R30 EnjuParser_T32 EnjuParser_T30 arg3Of ),(
EnjuParser_R31 EnjuParser_T25 EnjuParser_T33 arg1Of derivatization,of
EnjuParser_R32 EnjuParser_T38 EnjuParser_T33 arg2Of moiety,of
EnjuParser_R33 EnjuParser_T38 EnjuParser_T34 arg1Of moiety,a
EnjuParser_R34 EnjuParser_T38 EnjuParser_T35 arg1Of moiety,hydrophobic
EnjuParser_R35 EnjuParser_T38 EnjuParser_T36 arg1Of moiety,","
EnjuParser_R36 EnjuParser_T38 EnjuParser_T37 arg1Of moiety,aglycon
EnjuParser_R37 EnjuParser_T40 EnjuParser_T39 arg1Of linked,covalently
EnjuParser_R38 EnjuParser_T38 EnjuParser_T40 arg2Of moiety,linked
EnjuParser_R39 EnjuParser_T40 EnjuParser_T41 arg1Of linked,to
EnjuParser_R40 EnjuParser_T43 EnjuParser_T41 arg2Of number,to
EnjuParser_R41 EnjuParser_T43 EnjuParser_T42 arg1Of number,a
EnjuParser_R42 EnjuParser_T43 EnjuParser_T44 arg1Of number,of
EnjuParser_R43 EnjuParser_T46 EnjuParser_T44 arg2Of substrates,of
EnjuParser_R44 EnjuParser_T46 EnjuParser_T45 arg1Of substrates,acceptor
EnjuParser_R45 EnjuParser_T46 EnjuParser_T47 arg1Of substrates,(
EnjuParser_R46 EnjuParser_T48 EnjuParser_T47 arg2Of pNO2phenyl,(
EnjuParser_R47 EnjuParser_T49 EnjuParser_T47 arg3Of ),(
EnjuParser_R48 EnjuParser_T59 EnjuParser_T50 arg1Of we,Focusing
EnjuParser_R49 EnjuParser_T60 EnjuParser_T50 modOf found,Focusing
EnjuParser_R50 EnjuParser_T50 EnjuParser_T51 arg1Of Focusing,on
EnjuParser_R51 EnjuParser_T57 EnjuParser_T51 arg2Of N-acetyl-glucosaminyltransferase,on
EnjuParser_R52 EnjuParser_T57 EnjuParser_T52 arg1Of N-acetyl-glucosaminyltransferase,the
EnjuParser_R53 EnjuParser_T57 EnjuParser_T53 arg1Of N-acetyl-glucosaminyltransferase,Golgi
EnjuParser_R54 EnjuParser_T57 EnjuParser_T54 arg1Of N-acetyl-glucosaminyltransferase,enzyme
EnjuParser_R55 EnjuParser_T57 EnjuParser_T55 arg1Of N-acetyl-glucosaminyltransferase,core
EnjuParser_R56 EnjuParser_T57 EnjuParser_T56 arg1Of N-acetyl-glucosaminyltransferase,2
EnjuParser_R57 EnjuParser_T60 EnjuParser_T58 arg1Of found,","
EnjuParser_R58 EnjuParser_T59 EnjuParser_T60 arg1Of we,found
EnjuParser_R59 EnjuParser_T70 EnjuParser_T60 arg2Of were,found
EnjuParser_R60 EnjuParser_T70 EnjuParser_T61 arg1Of were,that
EnjuParser_R61 EnjuParser_T62 EnjuParser_T63 arg1Of synthesis,and
EnjuParser_R62 EnjuParser_T64 EnjuParser_T63 arg2Of fractionation,and
EnjuParser_R63 EnjuParser_T63 EnjuParser_T65 arg1Of and,of
EnjuParser_R64 EnjuParser_T69 EnjuParser_T65 arg2Of derivative,of
EnjuParser_R65 EnjuParser_T69 EnjuParser_T66 arg1Of derivative,the
EnjuParser_R66 EnjuParser_T69 EnjuParser_T67 arg2Of derivative,dansylated
EnjuParser_R67 EnjuParser_T69 EnjuParser_T68 arg1Of derivative,substrate
EnjuParser_R68 EnjuParser_T63 EnjuParser_T70 arg1Of and,were
EnjuParser_R69 EnjuParser_T74 EnjuParser_T70 arg2Of and,were
EnjuParser_R70 EnjuParser_T63 EnjuParser_T71 arg1Of and,rapid
EnjuParser_R71 EnjuParser_T71 EnjuParser_T72 arg1Of rapid,","
EnjuParser_R72 EnjuParser_T73 EnjuParser_T72 arg2Of easy,","
EnjuParser_R73 EnjuParser_T63 EnjuParser_T73 arg1Of and,easy
EnjuParser_R74 EnjuParser_T72 EnjuParser_T74 arg1Of ",",and
EnjuParser_R75 EnjuParser_T75 EnjuParser_T74 arg2Of inexpensive,and
EnjuParser_R76 EnjuParser_T63 EnjuParser_T75 arg1Of and,inexpensive
EnjuParser_R77 EnjuParser_T82 EnjuParser_T76 arg1Of proved,Additionally
EnjuParser_R78 EnjuParser_T82 EnjuParser_T77 arg1Of proved,","
EnjuParser_R79 EnjuParser_T81 EnjuParser_T78 arg1Of assay,the
EnjuParser_R80 EnjuParser_T81 EnjuParser_T79 arg1Of assay,corresponding
EnjuParser_R81 EnjuParser_T81 EnjuParser_T80 arg1Of assay,enzyme
EnjuParser_R82 EnjuParser_T81 EnjuParser_T82 arg1Of assay,proved
EnjuParser_R83 EnjuParser_T84 EnjuParser_T82 arg2Of and,proved
EnjuParser_R84 EnjuParser_T81 EnjuParser_T83 arg1Of assay,reproducible
EnjuParser_R85 EnjuParser_T83 EnjuParser_T84 arg1Of reproducible,and
EnjuParser_R86 EnjuParser_T86 EnjuParser_T84 arg2Of sensitive,and
EnjuParser_R87 EnjuParser_T86 EnjuParser_T85 arg1Of sensitive,very
EnjuParser_R88 EnjuParser_T81 EnjuParser_T86 arg1Of assay,sensitive
EnjuParser_R89 EnjuParser_T82 EnjuParser_T87 arg1Of proved,","
EnjuParser_R90 EnjuParser_T82 EnjuParser_T88 arg1Of proved,as
EnjuParser_R91 EnjuParser_T96 EnjuParser_T88 arg2Of detected,as
EnjuParser_R92 EnjuParser_T90 EnjuParser_T89 arg1Of pmol,0.4
EnjuParser_R93 EnjuParser_T90 EnjuParser_T91 arg1Of pmol,of
EnjuParser_R94 EnjuParser_T93 EnjuParser_T91 arg2Of product,of
EnjuParser_R95 EnjuParser_T93 EnjuParser_T92 arg1Of product,reaction
EnjuParser_R96 EnjuParser_T90 EnjuParser_T94 arg1Of pmol,were
EnjuParser_R97 EnjuParser_T96 EnjuParser_T94 arg2Of detected,were
EnjuParser_R98 EnjuParser_T96 EnjuParser_T95 arg1Of detected,readily
EnjuParser_R99 EnjuParser_T90 EnjuParser_T96 arg2Of pmol,detected
EnjuParser_R100 EnjuParser_T99 EnjuParser_T97 arg1Of approach,This
EnjuParser_R101 EnjuParser_T99 EnjuParser_T98 arg1Of approach,fluorometric
EnjuParser_R102 EnjuParser_T99 EnjuParser_T100 arg1Of approach,appears
EnjuParser_R103 EnjuParser_T103 EnjuParser_T100 arg2Of be,appears
EnjuParser_R104 EnjuParser_T100 EnjuParser_T101 arg1Of appears,therefore
EnjuParser_R105 EnjuParser_T103 EnjuParser_T102 arg1Of be,to
EnjuParser_R106 EnjuParser_T99 EnjuParser_T103 arg1Of approach,be
EnjuParser_R107 EnjuParser_T106 EnjuParser_T103 arg2Of tool,be
EnjuParser_R108 EnjuParser_T106 EnjuParser_T104 arg1Of tool,a
EnjuParser_R109 EnjuParser_T106 EnjuParser_T105 arg1Of tool,valid
EnjuParser_R110 EnjuParser_T106 EnjuParser_T107 arg1Of tool,for
EnjuParser_R111 EnjuParser_T108 EnjuParser_T107 arg2Of investigating,for
EnjuParser_R112 EnjuParser_T112 EnjuParser_T108 arg2Of expression,investigating
EnjuParser_R113 EnjuParser_T112 EnjuParser_T109 arg1Of expression,the
EnjuParser_R114 EnjuParser_T112 EnjuParser_T110 arg1Of expression,monitoring
EnjuParser_R115 EnjuParser_T112 EnjuParser_T111 arg1Of expression,differential
EnjuParser_R116 EnjuParser_T112 EnjuParser_T113 arg1Of expression,of
EnjuParser_R117 EnjuParser_T114 EnjuParser_T113 arg2Of glycosyltransferases,of
EnjuParser_R118 EnjuParser_T114 EnjuParser_T115 arg1Of glycosyltransferases,exhibiting
EnjuParser_R119 EnjuParser_T117 EnjuParser_T115 arg2Of levels,exhibiting
EnjuParser_R120 EnjuParser_T117 EnjuParser_T116 arg1Of levels,low
EnjuParser_R121 EnjuParser_T117 EnjuParser_T118 arg1Of levels,of
EnjuParser_R122 EnjuParser_T120 EnjuParser_T118 arg2Of activity,of
EnjuParser_R123 EnjuParser_T120 EnjuParser_T119 arg1Of activity,enzyme

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 388-394 Glycan denotes core 2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00033MO

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 388-394 Glycan denotes core 2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00033MO

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 375-380 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-77 Sentence denotes An approach for fluorometric determination of glycosyltransferase activities.
T2 78-174 Sentence denotes A new strategy for the fluorometric determination of glycosyltransferase activities is reported.
T3 175-358 Sentence denotes The method involves dansyl chloride derivatization of the reduced form (pNH2phenyl) of a hydrophobic, aglycon moiety covalently linked to a number of acceptor substrates (pNO2phenyl).
T4 359-543 Sentence denotes Focusing on the Golgi enzyme core 2 N-acetyl-glucosaminyltransferase, we found that synthesis and fractionation of the dansylated substrate derivative were rapid, easy and inexpensive.
T5 544-683 Sentence denotes Additionally, the corresponding enzyme assay proved reproducible and very sensitive, as 0.4 pmol of reaction product were readily detected.
T6 684-870 Sentence denotes This fluorometric approach appears therefore to be a valid tool for investigating the monitoring differential expression of glycosyltransferases exhibiting low levels of enzyme activity.

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 375-380 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794