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ngly1-sample3

Id Subject Object Predicate Lexical cue
T1 1012-1018 chem:24139 denotes GlcNAc
T2 1277-1283 chem:24139 denotes GlcNAc

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 117-136 Glycan_Motif denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48059CD|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G45827GY
T3 663-682 Glycan_Motif denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48059CD|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G45827GY
T5 1607-1626 Glycan_Motif denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48059CD|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G45827GY
T7 1884-1891 Glycan_Motif denotes glucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG

sentences

Id Subject Object Predicate Lexical cue
T1 0-201 Sentence denotes Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis.
T2 202-427 Sentence denotes The biosynthetic function of the lgtABE genetic locus of Neisseria meningitidis was determined by structural analysis of lipopolysaccharide (LPS) derived from mutant strains and enzymic assay for glycosyltransferase activity.
T3 428-612 Sentence denotes LPS was obtained from mutants generated by insertion of antibiotic resistance cassets in each of the three genes lgtA, lgtB, lgtE of the N. meningitidis immunotype L3 strain phi3 MC58.
T4 613-737 Sentence denotes LPS from the parent strain expresses the terminal lacto-N-neotetraose structure, Galbeta1-->4GlcNAcbeta1-->3Galbeta1-->4Glc.
T5 738-911 Sentence denotes Mild hydrazine treatment of the LPS afforded O-deacylated samples that were analyzed directly by electrospray ionization mass spectrometry (ESI-MS) in the negative ion mode.
T6 912-1073 Sentence denotes In conjunction with results from sugar analysis, ESI-MS revealed successive loss of the sugars Gal, GlcNAc, and Gal in lgt B, lgt A, and lgt E LPS, respectively.
T7 1074-1253 Sentence denotes The structure of a sample of O- and N-deacylated LPS derived by aqueous KOH treatment of lgt B LPS was determined in detail by two-dimensional homo- and heteronuclear NMR methods.
T8 1254-1423 Sentence denotes Using a synthetic beta-GlcNAc acceptor and a beta-lactose acceptor, the glycosyltransferase activities encoded by the lgtB and lgtA genes were unambiguously established.
T9 1424-1654 Sentence denotes These data provide the first definitive evidence that the three genes encode the respective glycosyltransferases required for biosynthesis of the terminal trisaccharide moiety of the lacto-N-neotetraose structure in Neisseria LPS.
T10 1655-1847 Sentence denotes From ESI-MS data, it was also determined that the Gal-deficient LPS expressed by the lgt E mutant is identical to that of the major component expressed by immunotype L3 galE-deficient strains.
T11 1848-1979 Sentence denotes The galE gene which encodes for UDP-glucose-4-epimerase plays an essential role in the incorporation of Gal into meningococcal LPS.
T1 0-201 Sentence denotes Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis.
T2 202-427 Sentence denotes The biosynthetic function of the lgtABE genetic locus of Neisseria meningitidis was determined by structural analysis of lipopolysaccharide (LPS) derived from mutant strains and enzymic assay for glycosyltransferase activity.
T3 428-612 Sentence denotes LPS was obtained from mutants generated by insertion of antibiotic resistance cassets in each of the three genes lgtA, lgtB, lgtE of the N. meningitidis immunotype L3 strain phi3 MC58.
T4 613-737 Sentence denotes LPS from the parent strain expresses the terminal lacto-N-neotetraose structure, Galbeta1-->4GlcNAcbeta1-->3Galbeta1-->4Glc.
T5 738-911 Sentence denotes Mild hydrazine treatment of the LPS afforded O-deacylated samples that were analyzed directly by electrospray ionization mass spectrometry (ESI-MS) in the negative ion mode.
T6 912-1073 Sentence denotes In conjunction with results from sugar analysis, ESI-MS revealed successive loss of the sugars Gal, GlcNAc, and Gal in lgt B, lgt A, and lgt E LPS, respectively.
T7 1074-1253 Sentence denotes The structure of a sample of O- and N-deacylated LPS derived by aqueous KOH treatment of lgt B LPS was determined in detail by two-dimensional homo- and heteronuclear NMR methods.
T8 1254-1423 Sentence denotes Using a synthetic beta-GlcNAc acceptor and a beta-lactose acceptor, the glycosyltransferase activities encoded by the lgtB and lgtA genes were unambiguously established.
T9 1424-1654 Sentence denotes These data provide the first definitive evidence that the three genes encode the respective glycosyltransferases required for biosynthesis of the terminal trisaccharide moiety of the lacto-N-neotetraose structure in Neisseria LPS.
T10 1655-1847 Sentence denotes From ESI-MS data, it was also determined that the Gal-deficient LPS expressed by the lgt E mutant is identical to that of the major component expressed by immunotype L3 galE-deficient strains.
T11 1848-1979 Sentence denotes The galE gene which encodes for UDP-glucose-4-epimerase plays an essential role in the incorporation of Gal into meningococcal LPS.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 117-136 https://glytoucan.org/Structures/Glycans/G45827GY denotes lacto-N-neotetraose
T2 117-136 https://glytoucan.org/Structures/Glycans/G48059CD denotes lacto-N-neotetraose
T3 663-682 https://glytoucan.org/Structures/Glycans/G45827GY denotes lacto-N-neotetraose
T4 663-682 https://glytoucan.org/Structures/Glycans/G48059CD denotes lacto-N-neotetraose
T5 1607-1626 https://glytoucan.org/Structures/Glycans/G45827GY denotes lacto-N-neotetraose
T6 1607-1626 https://glytoucan.org/Structures/Glycans/G48059CD denotes lacto-N-neotetraose
T7 1884-1891 https://glytoucan.org/Structures/Glycans/G15021LG denotes glucose

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 117-136 http://www.glycoepitope.jp/epitopes/EP0350 denotes lacto-N-neotetraose
T2 663-682 http://www.glycoepitope.jp/epitopes/EP0350 denotes lacto-N-neotetraose
T3 1607-1626 http://www.glycoepitope.jp/epitopes/EP0350 denotes lacto-N-neotetraose

NGLY1-deficiency

Id Subject Object Predicate Lexical cue
PD-NGLY1-deficiency-B_T1 1012-1018 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T2 1272-1283 chem:24139 denotes beta-GlcNAc
T1 1012-1018 chem:24139 denotes GlcNAc
T2 1272-1283 chem:24139 denotes beta-GlcNAc

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 117-136 Glycan denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/latest/png/binary/G48059CD
T2 663-682 Glycan denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/latest/png/binary/G48059CD
T3 1304-1311 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T4 1607-1626 Glycan denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/latest/png/binary/G48059CD

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 117-136 Glycan denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/latest/png/binary/G48059CD
T2 663-682 Glycan denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/latest/png/binary/G48059CD
T3 1304-1311 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T4 1607-1626 Glycan denotes lacto-N-neotetraose https://api.glycosmos.org/wurcs2image/latest/png/binary/G48059CD

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 117-136 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes lacto-N-neotetraose http://www.glycoepitope.jp/epitopes/EP0350
T2 663-682 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes lacto-N-neotetraose http://www.glycoepitope.jp/epitopes/EP0350
T3 1607-1626 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes lacto-N-neotetraose http://www.glycoepitope.jp/epitopes/EP0350

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 178-200 Organism denotes Neisseria meningitidis 487
T2 259-281 Organism denotes Neisseria meningitidis 487
T3 1640-1649 Organism denotes Neisseria 482

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-201 Sentence denotes Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis.
T2 202-427 Sentence denotes The biosynthetic function of the lgtABE genetic locus of Neisseria meningitidis was determined by structural analysis of lipopolysaccharide (LPS) derived from mutant strains and enzymic assay for glycosyltransferase activity.
T3 428-612 Sentence denotes LPS was obtained from mutants generated by insertion of antibiotic resistance cassets in each of the three genes lgtA, lgtB, lgtE of the N. meningitidis immunotype L3 strain phi3 MC58.
T4 613-737 Sentence denotes LPS from the parent strain expresses the terminal lacto-N-neotetraose structure, Galbeta1-->4GlcNAcbeta1-->3Galbeta1-->4Glc.
T5 738-911 Sentence denotes Mild hydrazine treatment of the LPS afforded O-deacylated samples that were analyzed directly by electrospray ionization mass spectrometry (ESI-MS) in the negative ion mode.
T6 912-1073 Sentence denotes In conjunction with results from sugar analysis, ESI-MS revealed successive loss of the sugars Gal, GlcNAc, and Gal in lgt B, lgt A, and lgt E LPS, respectively.
T7 1074-1253 Sentence denotes The structure of a sample of O- and N-deacylated LPS derived by aqueous KOH treatment of lgt B LPS was determined in detail by two-dimensional homo- and heteronuclear NMR methods.
T8 1254-1423 Sentence denotes Using a synthetic beta-GlcNAc acceptor and a beta-lactose acceptor, the glycosyltransferase activities encoded by the lgtB and lgtA genes were unambiguously established.
T9 1424-1654 Sentence denotes These data provide the first definitive evidence that the three genes encode the respective glycosyltransferases required for biosynthesis of the terminal trisaccharide moiety of the lacto-N-neotetraose structure in Neisseria LPS.
T10 1655-1847 Sentence denotes From ESI-MS data, it was also determined that the Gal-deficient LPS expressed by the lgt E mutant is identical to that of the major component expressed by immunotype L3 galE-deficient strains.
T11 1848-1979 Sentence denotes The galE gene which encodes for UDP-glucose-4-epimerase plays an essential role in the incorporation of Gal into meningococcal LPS.

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 117-136 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes lacto-N-neotetraose http://www.glycoepitope.jp/epitopes/EP0350
T2 663-682 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes lacto-N-neotetraose http://www.glycoepitope.jp/epitopes/EP0350
T3 1607-1626 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes lacto-N-neotetraose http://www.glycoepitope.jp/epitopes/EP0350

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 178-200 OrganismTaxon denotes Neisseria meningitidis 487
T2 259-281 OrganismTaxon denotes Neisseria meningitidis 487
T3 1217-1221 OrganismTaxon denotes homo 9605
T4 1640-1649 OrganismTaxon denotes Neisseria 482