Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-44 |
Sentence |
denotes |
Isocitrate dehydrogenase kinase/phosphatase. |
T2 |
45-110 |
Sentence |
denotes |
Kinetic characteristics of the wild-type and two mutant proteins. |
T3 |
111-225 |
Sentence |
denotes |
Isocitrate dehydrogenase (IDH) of Escherichia coli is regulated by a bifunctional protein, IDH kinase/phosphatase. |
T4 |
226-332 |
Sentence |
denotes |
In addition to the kinase and phosphatase activities, this protein catalyzes an intrinsic ATPase reaction. |
T5 |
333-416 |
Sentence |
denotes |
The initial velocity kinetics of these activities exhibited extensive similarities. |
T6 |
417-494 |
Sentence |
denotes |
IDH kinase and phosphatase both yielded intersecting double-reciprocal plots. |
T7 |
495-699 |
Sentence |
denotes |
In addition, we observed similar values for the kinetic constants describing interactions of the kinase and phosphatase with their protein substrates and the interactions of all three activities with ATP. |
T8 |
700-858 |
Sentence |
denotes |
In contrast, while the maximum velocities of IDH kinase and IDH phosphatase were nearly equal, they were 10-fold less than the maximum velocity of the ATPase. |
T9 |
859-1011 |
Sentence |
denotes |
Although the IDH phosphatase reaction required either ATP or ADP, it was not supported by the nonhydrolyzable ATP analogue 5'-adenylyl imidodiphosphate. |
T10 |
1012-1138 |
Sentence |
denotes |
The kinetic properties of wild-type IDH kinase/phosphatase were compared with those of two mutant derivatives of this protein. |
T11 |
1139-1216 |
Sentence |
denotes |
The mutations in these proteins selectively inhibit IDH phosphatase activity. |
T12 |
1217-1400 |
Sentence |
denotes |
Inhibition of IDH phosphatase resulted from three factors: decreases in the maximum velocities, reduced affinities for phospho-IDH, and a loss of coupling between ATP and phospho-IDH. |
T13 |
1401-1549 |
Sentence |
denotes |
These mutations also affected the properties of IDH kinase, increasing the maximum velocities and decreasing the affinities for ATP and phospho-IDH. |
T14 |
1550-1622 |
Sentence |
denotes |
The intrinsic ATPase activities also exhibited reduced affinity for ATP. |
T15 |
1623-1748 |
Sentence |
denotes |
These results are discussed in the context of a model which proposes that all three activities occur at the same active site. |
T1 |
0-44 |
Sentence |
denotes |
Isocitrate dehydrogenase kinase/phosphatase. |
T2 |
45-110 |
Sentence |
denotes |
Kinetic characteristics of the wild-type and two mutant proteins. |
T3 |
111-225 |
Sentence |
denotes |
Isocitrate dehydrogenase (IDH) of Escherichia coli is regulated by a bifunctional protein, IDH kinase/phosphatase. |
T4 |
226-332 |
Sentence |
denotes |
In addition to the kinase and phosphatase activities, this protein catalyzes an intrinsic ATPase reaction. |
T5 |
333-416 |
Sentence |
denotes |
The initial velocity kinetics of these activities exhibited extensive similarities. |
T6 |
417-494 |
Sentence |
denotes |
IDH kinase and phosphatase both yielded intersecting double-reciprocal plots. |
T7 |
495-699 |
Sentence |
denotes |
In addition, we observed similar values for the kinetic constants describing interactions of the kinase and phosphatase with their protein substrates and the interactions of all three activities with ATP. |
T8 |
700-858 |
Sentence |
denotes |
In contrast, while the maximum velocities of IDH kinase and IDH phosphatase were nearly equal, they were 10-fold less than the maximum velocity of the ATPase. |
T9 |
859-1011 |
Sentence |
denotes |
Although the IDH phosphatase reaction required either ATP or ADP, it was not supported by the nonhydrolyzable ATP analogue 5'-adenylyl imidodiphosphate. |
T10 |
1012-1138 |
Sentence |
denotes |
The kinetic properties of wild-type IDH kinase/phosphatase were compared with those of two mutant derivatives of this protein. |
T11 |
1139-1216 |
Sentence |
denotes |
The mutations in these proteins selectively inhibit IDH phosphatase activity. |
T12 |
1217-1400 |
Sentence |
denotes |
Inhibition of IDH phosphatase resulted from three factors: decreases in the maximum velocities, reduced affinities for phospho-IDH, and a loss of coupling between ATP and phospho-IDH. |
T13 |
1401-1549 |
Sentence |
denotes |
These mutations also affected the properties of IDH kinase, increasing the maximum velocities and decreasing the affinities for ATP and phospho-IDH. |
T14 |
1550-1622 |
Sentence |
denotes |
The intrinsic ATPase activities also exhibited reduced affinity for ATP. |
T15 |
1623-1748 |
Sentence |
denotes |
These results are discussed in the context of a model which proposes that all three activities occur at the same active site. |