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PubMed:8662939 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-93 Sentence denotes Identification of a critical ligand binding determinant of the human erythropoietin receptor.
T2 94-168 Sentence denotes Evidence for common ligand binding motifs in the cytokine receptor family.
T3 169-345 Sentence denotes The erythropoietin receptor (EPOR) is a member of a family of cytokine and growth factor receptors that share conserved features in their extracellular and cytoplasmic domains.
T4 346-504 Sentence denotes We have used site-specific mutagenesis within the extracellular domain of the EPOR to search for amino acid residues involved in erythropoietin (EPO) binding.
T5 505-661 Sentence denotes Mutant proteins were expressed in bacteria as soluble EPO binding proteins (EBP) and characterized for EPO binding activity in a number of different assays.
T6 662-802 Sentence denotes Substitution of phenylalanine at position 93 (Phe93) with alanine (F93A mutation) resulted in a drastic reduction in EPO binding in the EBP.
T7 803-921 Sentence denotes More conservative tyrosine or tryptophan substitutions at Phe93 resulted in much less dramatic effects on EPO binding.
T8 922-1034 Sentence denotes Biophysical studies indicated that the F93A mutation does not result in gross structural alterations in the EBP.
T9 1035-1142 Sentence denotes Furthermore, the F93A mutation in full-length EPOR expressed in COS cells abolished detectable EPO binding.
T10 1143-1263 Sentence denotes This was not a result of processing or transport defects, since mutant receptor was present on the surface of the cells.
T11 1264-1463 Sentence denotes Mutations in the region immediately around Phe93 and in residues homologous to other reported ligand binding determinants of the cytokine receptor family had small to moderate effects on EPO binding.
T12 1464-1545 Sentence denotes These data indicate that Phe93 is a critical EPO binding determinant of the EPOR.
T13 1546-1784 Sentence denotes Furthermore, since Phe93 aligns with critical ligand binding determinants in other receptors of the cytokine receptor family, these data suggest that receptors of this family may use common structural motifs to bind their cognate ligands.
T1 0-93 Sentence denotes Identification of a critical ligand binding determinant of the human erythropoietin receptor.
T2 94-168 Sentence denotes Evidence for common ligand binding motifs in the cytokine receptor family.
T3 169-345 Sentence denotes The erythropoietin receptor (EPOR) is a member of a family of cytokine and growth factor receptors that share conserved features in their extracellular and cytoplasmic domains.
T4 346-504 Sentence denotes We have used site-specific mutagenesis within the extracellular domain of the EPOR to search for amino acid residues involved in erythropoietin (EPO) binding.
T5 505-661 Sentence denotes Mutant proteins were expressed in bacteria as soluble EPO binding proteins (EBP) and characterized for EPO binding activity in a number of different assays.
T6 662-802 Sentence denotes Substitution of phenylalanine at position 93 (Phe93) with alanine (F93A mutation) resulted in a drastic reduction in EPO binding in the EBP.
T7 803-921 Sentence denotes More conservative tyrosine or tryptophan substitutions at Phe93 resulted in much less dramatic effects on EPO binding.
T8 922-1034 Sentence denotes Biophysical studies indicated that the F93A mutation does not result in gross structural alterations in the EBP.
T9 1035-1142 Sentence denotes Furthermore, the F93A mutation in full-length EPOR expressed in COS cells abolished detectable EPO binding.
T10 1143-1263 Sentence denotes This was not a result of processing or transport defects, since mutant receptor was present on the surface of the cells.
T11 1264-1463 Sentence denotes Mutations in the region immediately around Phe93 and in residues homologous to other reported ligand binding determinants of the cytokine receptor family had small to moderate effects on EPO binding.
T12 1464-1545 Sentence denotes These data indicate that Phe93 is a critical EPO binding determinant of the EPOR.
T13 1546-1784 Sentence denotes Furthermore, since Phe93 aligns with critical ligand binding determinants in other receptors of the cytokine receptor family, these data suggest that receptors of this family may use common structural motifs to bind their cognate ligands.

AIMed

Id Subject Object Predicate Lexical cue
T1 69-92 protein denotes erythropoietin receptor
T2 173-196 protein denotes erythropoietin receptor
T3 198-202 protein denotes EPOR
T4 424-428 protein denotes EPOR
T5 475-489 protein denotes erythropoietin
T6 491-494 protein denotes EPO
T7 559-562 protein denotes EPO
T8 581-584 protein denotes EBP
T9 608-611 protein denotes EPO
T10 779-782 protein denotes EPO
T11 798-801 protein denotes EBP
T12 909-912 protein denotes EPO
T13 1030-1033 protein denotes EBP
T14 1081-1085 protein denotes EPOR
T15 1130-1133 protein denotes EPO
T16 1451-1454 protein denotes EPO
T17 1509-1512 protein denotes EPO
T18 1540-1544 protein denotes EPOR

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 63-68 OrganismTaxon denotes human 9606
T2 539-547 OrganismTaxon denotes bacteria 2|629395

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 307-320 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576
T2 325-336 Body_part denotes cytoplasmic http://purl.obolibrary.org/obo/GO_0005737
T3 396-409 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576