| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-145 |
Sentence |
denotes |
A conserved domain of the viviparous-1 gene product enhances the DNA binding activity of the bZIP protein EmBP-1 and other transcription factors. |
| T2 |
146-356 |
Sentence |
denotes |
The maize VP1 protein is a seed-specific regulator of gene expression that effects the expression of a subset of abscisic acid (ABA)-regulated genes that are expressed during the maturation program of the seed. |
| T3 |
357-446 |
Sentence |
denotes |
In addition, VP1 has pleiotropic effects on seed development that are not related to ABA. |
| T4 |
447-669 |
Sentence |
denotes |
In transient expression assays, VP1 has been shown to transactivate gene expression through at least two distinct promoter elements: the G boxes from the ABA-inducible wheat Em gene and the SphI box from the maize C1 gene. |
| T5 |
670-854 |
Sentence |
denotes |
We have investigated how VP1 can transactivate gene expression through diverse promoter elements by analyzing its association in vitro with EmBP-1, a factor that binds the Em promoter. |
| T6 |
855-961 |
Sentence |
denotes |
We demonstrate that VP1 can greatly enhance the DNA binding activity of EmBP-1 in a gel retardation assay. |
| T7 |
962-1082 |
Sentence |
denotes |
This enhancing activity has also been observed on transcription factors as diverse as Opaque-2, Max, Sp1, and NF-kappaB. |
| T8 |
1083-1278 |
Sentence |
denotes |
Deletion of a small but highly conserved region (BR2) in VP1 eliminates the enhancement in vitro as well as the ability of VP1 to transactivate Em gene expression in a transient expression assay. |
| T9 |
1279-1437 |
Sentence |
denotes |
A 40-amino acid fragment from VP1 sandwiched between the maltose-binding protein and LacZ can confer the enhancement function to this fusion protein in vitro. |
| T10 |
1438-1540 |
Sentence |
denotes |
A weak and relatively nonspecific interaction between BR2 and DNA is demonstrated by UV cross-linking. |
| T11 |
1541-1714 |
Sentence |
denotes |
The in vitro properties we observe for VP1 might explain the regulatory effects of VP1 on a diverse set of genes and why mutations in the vp1 locus have pleiotropic effects. |
| T1 |
0-145 |
Sentence |
denotes |
A conserved domain of the viviparous-1 gene product enhances the DNA binding activity of the bZIP protein EmBP-1 and other transcription factors. |
| T2 |
146-356 |
Sentence |
denotes |
The maize VP1 protein is a seed-specific regulator of gene expression that effects the expression of a subset of abscisic acid (ABA)-regulated genes that are expressed during the maturation program of the seed. |
| T3 |
357-446 |
Sentence |
denotes |
In addition, VP1 has pleiotropic effects on seed development that are not related to ABA. |
| T4 |
447-669 |
Sentence |
denotes |
In transient expression assays, VP1 has been shown to transactivate gene expression through at least two distinct promoter elements: the G boxes from the ABA-inducible wheat Em gene and the SphI box from the maize C1 gene. |
| T5 |
670-854 |
Sentence |
denotes |
We have investigated how VP1 can transactivate gene expression through diverse promoter elements by analyzing its association in vitro with EmBP-1, a factor that binds the Em promoter. |
| T6 |
855-961 |
Sentence |
denotes |
We demonstrate that VP1 can greatly enhance the DNA binding activity of EmBP-1 in a gel retardation assay. |
| T7 |
962-1082 |
Sentence |
denotes |
This enhancing activity has also been observed on transcription factors as diverse as Opaque-2, Max, Sp1, and NF-kappaB. |
| T8 |
1083-1278 |
Sentence |
denotes |
Deletion of a small but highly conserved region (BR2) in VP1 eliminates the enhancement in vitro as well as the ability of VP1 to transactivate Em gene expression in a transient expression assay. |
| T9 |
1279-1437 |
Sentence |
denotes |
A 40-amino acid fragment from VP1 sandwiched between the maltose-binding protein and LacZ can confer the enhancement function to this fusion protein in vitro. |
| T10 |
1438-1540 |
Sentence |
denotes |
A weak and relatively nonspecific interaction between BR2 and DNA is demonstrated by UV cross-linking. |
| T11 |
1541-1714 |
Sentence |
denotes |
The in vitro properties we observe for VP1 might explain the regulatory effects of VP1 on a diverse set of genes and why mutations in the vp1 locus have pleiotropic effects. |