PubMed:8563133
Annnotations
Glycan-Motif
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 648-659 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
| T2 | 672-693 | https://glytoucan.org/Structures/Glycans/G27025MB | denotes | N-acetylgalactosamine |
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 648-659 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
| T2 | 672-693 | Glycan_Motif | denotes | N-acetylgalactosamine | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G27025MB |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 648-659 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
| T2 | 672-693 | https://glytoucan.org/Structures/Glycans/G27025MB | denotes | N-acetylgalactosamine |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 100-107 | http://purl.obolibrary.org/obo/MAT_0000081 | denotes | thyroid |
| T2 | 223-230 | http://purl.obolibrary.org/obo/MAT_0000081 | denotes | thyroid |
| T3 | 417-426 | http://purl.obolibrary.org/obo/MAT_0000077 | denotes | pituitary |
| T4 | 1047-1054 | http://purl.obolibrary.org/obo/MAT_0000081 | denotes | thyroid |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| TextSentencer_T1 | 0-81 | Sentence | denotes | Biological and immunochemical characterization of recombinant human thyrotrophin. |
| TextSentencer_T2 | 82-238 | Sentence | denotes | Recombinant human thyroid-stimulating hormone (recTSH) has recently been engineered to detect metastatic lesions in patients operated on for thyroid cancer. |
| TextSentencer_T3 | 239-443 | Sentence | denotes | In this report, we have compared the microheterogeneity, carbohydrate (CHO) content, mitogenic potency and immunoreactivity of the biotechnology product to those of human TSH of pituitary origin (pitTSH). |
| TextSentencer_T4 | 444-694 | Sentence | denotes | Compositional analysis revealed that recombinant (rec) TSH produced in Chinese hamster ovary cells was overglycosylated compared with the native hormone (21 and 14%, respectively) with a higher amount of sialic acid and lack of N-acetylgalactosamine. |
| TextSentencer_T5 | 695-999 | Sentence | denotes | Electrofocusing followed by immunoblotting resolved recTSH into six glycoforms with pIs ranging from 6.0 to 8.6, which were converted to a major species of pI 8.9 by sialidase treatment. pitTSH contained five main isoforms of pI 6.5-8.2 distinct from those of recTSH and partially resistant to sialidase. |
| TextSentencer_T6 | 1000-1220 | Sentence | denotes | Binding activity of both human TSHs to porcine thyroid membrane receptors was found to be similar, but recTSH appeared to be 20% active compared to pitTSH in eliciting cAMP production and cell growth in rat FRTL-5 cells. |
| TextSentencer_T7 | 1221-1406 | Sentence | denotes | Immunoreactivity of the recombinant hormone was investigated using polyclonal and monoclonal antibodies raised against the native hormone or synthetic peptide sequences of its subunits. |
| TextSentencer_T8 | 1407-1562 | Sentence | denotes | While rec- and pitTSH were recognized to a similar extent by anti-protein antibodies, they exhibited a different binding pattern to antipeptide antibodies. |
| TextSentencer_T9 | 1563-1819 | Sentence | denotes | Serial dilution of anti-alpha 1-25, anti-alpha 26-51, anti-beta 96-112 antisera bound recTSH to a greater extent than pitTSH, while anti-beta 31-51 and anti-beta 53-76 displayed similar recognition toward both preparations.(ABSTRACT TRUNCATED AT 250 WORDS) |
| T1 | 0-81 | Sentence | denotes | Biological and immunochemical characterization of recombinant human thyrotrophin. |
| T2 | 82-238 | Sentence | denotes | Recombinant human thyroid-stimulating hormone (recTSH) has recently been engineered to detect metastatic lesions in patients operated on for thyroid cancer. |
| T3 | 239-443 | Sentence | denotes | In this report, we have compared the microheterogeneity, carbohydrate (CHO) content, mitogenic potency and immunoreactivity of the biotechnology product to those of human TSH of pituitary origin (pitTSH). |
| T4 | 444-694 | Sentence | denotes | Compositional analysis revealed that recombinant (rec) TSH produced in Chinese hamster ovary cells was overglycosylated compared with the native hormone (21 and 14%, respectively) with a higher amount of sialic acid and lack of N-acetylgalactosamine. |
| T5 | 695-999 | Sentence | denotes | Electrofocusing followed by immunoblotting resolved recTSH into six glycoforms with pIs ranging from 6.0 to 8.6, which were converted to a major species of pI 8.9 by sialidase treatment. pitTSH contained five main isoforms of pI 6.5-8.2 distinct from those of recTSH and partially resistant to sialidase. |
| T6 | 1000-1220 | Sentence | denotes | Binding activity of both human TSHs to porcine thyroid membrane receptors was found to be similar, but recTSH appeared to be 20% active compared to pitTSH in eliciting cAMP production and cell growth in rat FRTL-5 cells. |
| T7 | 1221-1406 | Sentence | denotes | Immunoreactivity of the recombinant hormone was investigated using polyclonal and monoclonal antibodies raised against the native hormone or synthetic peptide sequences of its subunits. |
| T8 | 1407-1562 | Sentence | denotes | While rec- and pitTSH were recognized to a similar extent by anti-protein antibodies, they exhibited a different binding pattern to antipeptide antibodies. |
| T9 | 1563-1819 | Sentence | denotes | Serial dilution of anti-alpha 1-25, anti-alpha 26-51, anti-beta 96-112 antisera bound recTSH to a greater extent than pitTSH, while anti-beta 31-51 and anti-beta 53-76 displayed similar recognition toward both preparations.(ABSTRACT TRUNCATED AT 250 WORDS) |
| T1 | 0-81 | Sentence | denotes | Biological and immunochemical characterization of recombinant human thyrotrophin. |
| T2 | 82-238 | Sentence | denotes | Recombinant human thyroid-stimulating hormone (recTSH) has recently been engineered to detect metastatic lesions in patients operated on for thyroid cancer. |
| T3 | 239-443 | Sentence | denotes | In this report, we have compared the microheterogeneity, carbohydrate (CHO) content, mitogenic potency and immunoreactivity of the biotechnology product to those of human TSH of pituitary origin (pitTSH). |
| T4 | 444-694 | Sentence | denotes | Compositional analysis revealed that recombinant (rec) TSH produced in Chinese hamster ovary cells was overglycosylated compared with the native hormone (21 and 14%, respectively) with a higher amount of sialic acid and lack of N-acetylgalactosamine. |
| T5 | 695-999 | Sentence | denotes | Electrofocusing followed by immunoblotting resolved recTSH into six glycoforms with pIs ranging from 6.0 to 8.6, which were converted to a major species of pI 8.9 by sialidase treatment. pitTSH contained five main isoforms of pI 6.5-8.2 distinct from those of recTSH and partially resistant to sialidase. |
| T6 | 1000-1220 | Sentence | denotes | Binding activity of both human TSHs to porcine thyroid membrane receptors was found to be similar, but recTSH appeared to be 20% active compared to pitTSH in eliciting cAMP production and cell growth in rat FRTL-5 cells. |
| T7 | 1221-1406 | Sentence | denotes | Immunoreactivity of the recombinant hormone was investigated using polyclonal and monoclonal antibodies raised against the native hormone or synthetic peptide sequences of its subunits. |
| T8 | 1407-1562 | Sentence | denotes | While rec- and pitTSH were recognized to a similar extent by anti-protein antibodies, they exhibited a different binding pattern to antipeptide antibodies. |
| T9 | 1563-1819 | Sentence | denotes | Serial dilution of anti-alpha 1-25, anti-alpha 26-51, anti-beta 96-112 antisera bound recTSH to a greater extent than pitTSH, while anti-beta 31-51 and anti-beta 53-76 displayed similar recognition toward both preparations.(ABSTRACT TRUNCATED AT 250 WORDS) |
ICD10
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 100-107 | http://purl.bioontology.org/ontology/ICD10/E06 | denotes | thyroid |
| T2 | 223-230 | http://purl.bioontology.org/ontology/ICD10/E06 | denotes | thyroid |
| T3 | 1047-1054 | http://purl.bioontology.org/ontology/ICD10/E06 | denotes | thyroid |
| T4 | 100-107 | http://purl.bioontology.org/ontology/ICD10/E06.9 | denotes | thyroid |
| T5 | 223-230 | http://purl.bioontology.org/ontology/ICD10/E06.9 | denotes | thyroid |
| T6 | 1047-1054 | http://purl.bioontology.org/ontology/ICD10/E06.9 | denotes | thyroid |
GlycoBiology-FMA
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 68-80 | FMAID:74640 | denotes | thyrotrophin |
| _T2 | 68-80 | FMAID:179406 | denotes | thyrotrophin |
| _T3 | 68-80 | FMAID:83099 | denotes | thyrotrophin |
| _T4 | 68-80 | FMAID:197368 | denotes | thyrotrophin |
| _T5 | 100-107 | FMAID:13344 | denotes | thyroid |
| _T6 | 100-107 | FMAID:102701 | denotes | thyroid |
| _T7 | 120-127 | FMAID:12278 | denotes | hormone |
| _T8 | 120-127 | FMAID:101015 | denotes | hormone |
| _T9 | 223-230 | FMAID:102701 | denotes | thyroid |
| _T10 | 223-230 | FMAID:13344 | denotes | thyroid |
| _T11 | 296-308 | FMAID:82737 | denotes | carbohydrate |
| _T12 | 296-308 | FMAID:197276 | denotes | carbohydrate |
| _T13 | 417-426 | FMAID:164956 | denotes | pituitary |
| _T14 | 444-457 | FMAID:146309 | denotes | Compositional |
| _T15 | 444-457 | FMAID:50603 | denotes | Compositional |
| _T16 | 531-536 | FMAID:93687 | denotes | ovary |
| _T17 | 531-536 | FMAID:7209 | denotes | ovary |
| _T18 | 537-542 | FMAID:68646 | denotes | cells |
| _T19 | 537-542 | FMAID:169002 | denotes | cells |
| _T20 | 589-596 | FMAID:101015 | denotes | hormone |
| _T21 | 589-596 | FMAID:12278 | denotes | hormone |
| _T22 | 672-693 | FMAID:196780 | denotes | N-acetylgalactosamine |
| _T23 | 672-693 | FMAID:82786 | denotes | N-acetylgalactosamine |
| _T24 | 779-782 | FMAID:214745 | denotes | pIs |
| _T25 | 851-853 | FMAID:214745 | denotes | pI |
| _T26 | 904-908 | FMAID:214734 | denotes | main |
| _T27 | 921-923 | FMAID:214745 | denotes | pI |
| _T28 | 1047-1054 | FMAID:13344 | denotes | thyroid |
| _T29 | 1047-1054 | FMAID:102701 | denotes | thyroid |
| _T30 | 1047-1063 | FMAID:55132 | denotes | thyroid membrane |
| _T31 | 1047-1063 | FMAID:151710 | denotes | thyroid membrane |
| _T32 | 1184-1192 | FMAID:86471 | denotes | and cell |
| _T33 | 1184-1192 | FMAID:200932 | denotes | and cell |
| _T34 | 1214-1219 | FMAID:68646 | denotes | cells |
| _T35 | 1214-1219 | FMAID:169002 | denotes | cells |
| _T36 | 1257-1264 | FMAID:101015 | denotes | hormone |
| _T37 | 1257-1264 | FMAID:12278 | denotes | hormone |
| _T38 | 1314-1324 | FMAID:167180 | denotes | antibodies |
| _T39 | 1351-1358 | FMAID:101015 | denotes | hormone |
| _T40 | 1351-1358 | FMAID:12278 | denotes | hormone |
| _T41 | 1473-1480 | FMAID:165447 | denotes | protein |
| _T42 | 1473-1480 | FMAID:67257 | denotes | protein |
| _T43 | 1481-1491 | FMAID:167180 | denotes | antibodies |
| _T44 | 1551-1561 | FMAID:167180 | denotes | antibodies |
uniprot-human
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 494-497 | http://www.uniprot.org/uniprot/Q9UIJ5 | denotes | rec |
| T2 | 1413-1416 | http://www.uniprot.org/uniprot/Q9UIJ5 | denotes | rec |
uniprot-mouse
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1806-1808 | http://www.uniprot.org/uniprot/Q8VHK8 | denotes | AT |
GlycoBiology-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 120-127 | http://purl.bioontology.org/ontology/STY/T125 | denotes | hormone |
| T2 | 231-237 | http://purl.bioontology.org/ontology/NCBITAXON/6754 | denotes | cancer |
| T3 | 537-542 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
| T4 | 589-596 | http://purl.bioontology.org/ontology/STY/T125 | denotes | hormone |
| T5 | 1064-1073 | http://purl.bioontology.org/ontology/STY/T192 | denotes | receptors |
| T6 | 1214-1219 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
| T7 | 1257-1264 | http://purl.bioontology.org/ontology/STY/T125 | denotes | hormone |
| T8 | 1303-1313 | http://purl.bioontology.org/ontology/NCBITAXON/608684 | denotes | monoclonal |
| T9 | 1351-1358 | http://purl.bioontology.org/ontology/STY/T125 | denotes | hormone |
| T10 | 1622-1626 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
| T11 | 1622-1626 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
| T12 | 1700-1704 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
| T13 | 1700-1704 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
| T14 | 1720-1724 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
| T15 | 1720-1724 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 100-127 | http://purl.obolibrary.org/obo/GO_0070460 | denotes | thyroid-stimulating hormone |
| T2 | 100-127 | http://purl.obolibrary.org/obo/GO_1904400 | denotes | thyroid-stimulating hormone |
| T3 | 310-313 | http://purl.obolibrary.org/obo/GO_0043848 | denotes | CHO |
| T4 | 1000-1016 | http://purl.obolibrary.org/obo/GO_0051099 | denotes | Binding activity |
| T5 | 1188-1199 | http://purl.obolibrary.org/obo/GO_0016049 | denotes | cell growth |
| T6 | 1193-1199 | http://purl.obolibrary.org/obo/GO_0040007 | denotes | growth |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1000-1007 | http://purl.obolibrary.org/obo/GO_0070026 | denotes | Binding |
| T2 | 1520-1527 | http://purl.obolibrary.org/obo/GO_0070026 | denotes | binding |
| T3 | 1000-1007 | http://purl.obolibrary.org/obo/GO_0003680 | denotes | Binding |
| T4 | 1520-1527 | http://purl.obolibrary.org/obo/GO_0003680 | denotes | binding |
| T5 | 1000-1007 | http://purl.obolibrary.org/obo/GO_0017091 | denotes | Binding |
| T6 | 1520-1527 | http://purl.obolibrary.org/obo/GO_0017091 | denotes | binding |
| T7 | 1000-1007 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | Binding |
| T8 | 1520-1527 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | binding |
| T9 | 1314-1324 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibodies |
| T10 | 1481-1491 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibodies |
| T11 | 1551-1561 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibodies |
| T12 | 1520-1535 | http://purl.obolibrary.org/obo/GO_0001871 | denotes | binding pattern |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 537-542 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T2 | 1188-1192 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T3 | 1055-1063 | http://purl.obolibrary.org/obo/GO_0016020 | denotes | membrane |
EDAM-topics
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 62-67 | http://edamontology.org/topic_2815 | denotes | human |
| T2 | 94-99 | http://edamontology.org/topic_2815 | denotes | human |
| T3 | 231-237 | http://edamontology.org/topic_2640 | denotes | cancer |
| T4 | 296-308 | http://edamontology.org/topic_0152 | denotes | carbohydrate |
| T5 | 370-383 | http://edamontology.org/topic_3297 | denotes | biotechnology |
| T6 | 404-409 | http://edamontology.org/topic_2815 | denotes | human |
| T7 | 1025-1030 | http://edamontology.org/topic_2815 | denotes | human |
| T8 | 1372-1379 | http://edamontology.org/topic_0154 | denotes | peptide |
| T9 | 1380-1389 | http://edamontology.org/topic_0080 | denotes | sequences |
| T10 | 1380-1389 | http://edamontology.org/topic_3168 | denotes | sequences |
| T11 | 1473-1480 | http://edamontology.org/topic_0078 | denotes | protein |
EDAM-DFO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 169-175 | http://edamontology.org/operation_2423 | denotes | detect |
| T2 | 247-253 | http://edamontology.org/data_2048 | denotes | report |
| T3 | 458-466 | http://edamontology.org/operation_2945 | denotes | analysis |
| T4 | 1372-1379 | http://edamontology.org/data_2906 | denotes | peptide |
| T5 | 1380-1389 | http://edamontology.org/data_2044 | denotes | sequences |
| T6 | 1380-1389 | http://edamontology.org/operation_3218 | denotes | sequences |
| T7 | 1473-1480 | http://edamontology.org/format_1208 | denotes | protein |
| T8 | 1473-1480 | http://edamontology.org/data_1467 | denotes | protein |
| T9 | 1749-1760 | http://edamontology.org/operation_2423 | denotes | recognition |
| T10 | 1787-1795 | http://edamontology.org/data_2849 | denotes | ABSTRACT |
GlycoBiology-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 100-107 | http://purl.obolibrary.org/obo/MAT_0000081 | denotes | thyroid |
| T2 | 223-230 | http://purl.obolibrary.org/obo/MAT_0000081 | denotes | thyroid |
| T3 | 417-426 | http://purl.obolibrary.org/obo/MAT_0000077 | denotes | pituitary |
| T4 | 1047-1054 | http://purl.obolibrary.org/obo/MAT_0000081 | denotes | thyroid |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 100-107 | Body_part | denotes | thyroid | http://purl.obolibrary.org/obo/MAT_0000081 |
| T2 | 223-230 | Body_part | denotes | thyroid | http://purl.obolibrary.org/obo/MAT_0000081 |
| T3 | 417-426 | Body_part | denotes | pituitary | http://purl.obolibrary.org/obo/MAT_0000077 |
| T4 | 1047-1054 | Body_part | denotes | thyroid | http://purl.obolibrary.org/obo/MAT_0000081 |
HP-phenotype
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 231-237 | Phenotype | denotes | cancer | HP:0002664 |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 223-237 | Disease | denotes | thyroid cancer | http://purl.obolibrary.org/obo/MONDO_0002108|http://purl.obolibrary.org/obo/MONDO_0015075 |
| T3 | 417-426 | Disease | denotes | pituitary | http://purl.obolibrary.org/obo/MONDO_0021156 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 62-67 | OrganismTaxon | denotes | human | 9606 |
| T2 | 94-99 | OrganismTaxon | denotes | human | 9606 |
| T3 | 404-409 | OrganismTaxon | denotes | human | 9606 |
| T4 | 515-530 | OrganismTaxon | denotes | Chinese hamster | 10029 |
| T5 | 1025-1030 | OrganismTaxon | denotes | human | 9606 |
| T6 | 1203-1206 | OrganismTaxon | denotes | rat | 10114|10116 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 100-107 | Body_part | denotes | thyroid | http://purl.obolibrary.org/obo/UBERON_0002046 |
| T2 | 223-230 | Body_part | denotes | thyroid | http://purl.obolibrary.org/obo/UBERON_0002046 |
| T3 | 417-426 | Body_part | denotes | pituitary | http://purl.obolibrary.org/obo/UBERON_0000007 |
| T4 | 531-536 | Body_part | denotes | ovary | http://purl.obolibrary.org/obo/UBERON_0000992 |
| T5 | 1047-1054 | Body_part | denotes | thyroid | http://purl.obolibrary.org/obo/UBERON_0002046 |
| T6 | 1055-1063 | Body_part | denotes | membrane | http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158 |