> top > docs > PubMed:8486686 > annotations

PubMed:8486686 JSONTXT

Annnotations TAB JSON ListView MergeView

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 190-193 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T3 212-215 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK
T5 799-802 Glycan_Motif denotes GM2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC
T7 803-806 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK
T9 872-875 Glycan_Motif denotes GM3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G91237TK|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G56516VH
T11 880-883 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T13 912-915 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T15 966-969 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T17 1230-1233 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T19 1237-1240 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T21 1367-1370 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T23 1841-1847 Glycan_Motif denotes Neu5Ac https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T24 1983-1986 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T26 2031-2037 Glycan_Motif denotes Neu5Ac https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T27 2065-2068 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK
T29 2073-2076 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T31 2259-2262 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK
T33 2377-2380 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK
T35 2400-2403 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T37 2455-2458 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK
T39 2502-2505 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK
T41 2527-2541 Glycan_Motif denotes 9-O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G74121RX
T42 2538-2541 Glycan_Motif denotes GD3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ
T44 2546-2549 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK
T46 2582-2585 Glycan_Motif denotes GD2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK

sentences

Id Subject Object Predicate Lexical cue
T1 0-131 Sentence denotes Kinetic and spatial interrelationships between ganglioside glycosyltransferases and O-acetyltransferase(s) in human melanoma cells.
T2 132-258 Sentence denotes The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized.
T3 259-407 Sentence denotes However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure.
T4 408-617 Sentence denotes Here, we have studied the spatial and temporal interrelationships among the activities responsible for the later stages of ganglioside biosynthesis and those for O-acetylation in cultured human melanoma cells.
T5 618-767 Sentence denotes First, brefeldin A treatment was used to separate biosynthetic steps into compartments distal or proximal to the transport block imposed by the drug.
T6 768-884 Sentence denotes In keeping with prior reports, GM2/GD2 synthase was consistently rendered inaccessible to its acceptors GM3 and GD3.
T7 885-952 Sentence denotes In contrast, the effect on GD3 biosynthesis was cell line-specific.
T8 953-1042 Sentence denotes Synthesis of GD3 was nearly abrogated in two lines, while it accumulated in a third line.
T9 1043-1166 Sentence denotes This indicates that the spatial organization of ganglioside processing activities can vary even between similar cell lines.
T10 1167-1429 Sentence denotes However, in all cell lines studied, the ratio of 9(7)-O-acetyl-GD3 to GD3 was not changed by brefeldin A, indicating that the majority of ganglioside O-acetyltransferase activity is co-localized with GD3 biosynthetic activity in the same Golgi subcompartment(s).
T11 1430-1583 Sentence denotes As an alternative approach, Golgi-enriched fractions from melanoma cells were incubated with radiolabeled and nonlabeled nucleotide sugars or acetyl-CoA.
T12 1584-1819 Sentence denotes In these preparations, biosynthesis is dependent upon the co-localization of appropriate sugar nucleotide transporters, glycosyltransferases, and acceptors that are endogenously present within intact topologically correct compartments.
T13 1820-2012 Sentence denotes Incubations with CMP-Neu5Ac and acetyl-CoA corroborated the results with brefeldin A, co-localizing ganglioside O-acetyltransferase activity in compartments where GD3 biosynthesis takes place.
T14 2013-2131 Sentence denotes Analyses with CMP-Neu5Ac and UDP-GalNAc showed that GD2 and GD3 synthesis occur in partially overlapping compartments.
T15 2132-2381 Sentence denotes Labeling with acetyl-CoA and UDP-GalNAc indicated that although labeled acetate can be transferred from acetyl-CoA directly to GD2, ganglioside O-acetyltransferase activity does not substantially overlap with the biosynthetic compartment(s) for GD2.
T16 2382-2515 Sentence denotes Instead, O-acetyl-GD3 appears to be co-localized with the compartment of GD2 biosynthesis and serves as an acceptor for GD2 synthase.
T17 2516-2627 Sentence denotes Thus, both 9-O-acetyl-GD3 and GD2 can be precursors of 9-O-acetyl-GD2, but apparently in distinct compartments.
T1 0-131 Sentence denotes Kinetic and spatial interrelationships between ganglioside glycosyltransferases and O-acetyltransferase(s) in human melanoma cells.
T2 132-258 Sentence denotes The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized.
T3 259-407 Sentence denotes However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure.
T4 408-617 Sentence denotes Here, we have studied the spatial and temporal interrelationships among the activities responsible for the later stages of ganglioside biosynthesis and those for O-acetylation in cultured human melanoma cells.
T5 618-767 Sentence denotes First, brefeldin A treatment was used to separate biosynthetic steps into compartments distal or proximal to the transport block imposed by the drug.
T6 768-884 Sentence denotes In keeping with prior reports, GM2/GD2 synthase was consistently rendered inaccessible to its acceptors GM3 and GD3.
T7 885-952 Sentence denotes In contrast, the effect on GD3 biosynthesis was cell line-specific.
T8 953-1042 Sentence denotes Synthesis of GD3 was nearly abrogated in two lines, while it accumulated in a third line.
T9 1043-1166 Sentence denotes This indicates that the spatial organization of ganglioside processing activities can vary even between similar cell lines.
T10 1167-1429 Sentence denotes However, in all cell lines studied, the ratio of 9(7)-O-acetyl-GD3 to GD3 was not changed by brefeldin A, indicating that the majority of ganglioside O-acetyltransferase activity is co-localized with GD3 biosynthetic activity in the same Golgi subcompartment(s).
T11 1430-1583 Sentence denotes As an alternative approach, Golgi-enriched fractions from melanoma cells were incubated with radiolabeled and nonlabeled nucleotide sugars or acetyl-CoA.
T12 1584-1819 Sentence denotes In these preparations, biosynthesis is dependent upon the co-localization of appropriate sugar nucleotide transporters, glycosyltransferases, and acceptors that are endogenously present within intact topologically correct compartments.
T13 1820-2012 Sentence denotes Incubations with CMP-Neu5Ac and acetyl-CoA corroborated the results with brefeldin A, co-localizing ganglioside O-acetyltransferase activity in compartments where GD3 biosynthesis takes place.
T14 2013-2131 Sentence denotes Analyses with CMP-Neu5Ac and UDP-GalNAc showed that GD2 and GD3 synthesis occur in partially overlapping compartments.
T15 2132-2381 Sentence denotes Labeling with acetyl-CoA and UDP-GalNAc indicated that although labeled acetate can be transferred from acetyl-CoA directly to GD2, ganglioside O-acetyltransferase activity does not substantially overlap with the biosynthetic compartment(s) for GD2.
T16 2382-2515 Sentence denotes Instead, O-acetyl-GD3 appears to be co-localized with the compartment of GD2 biosynthesis and serves as an acceptor for GD2 synthase.
T17 2516-2627 Sentence denotes Thus, both 9-O-acetyl-GD3 and GD2 can be precursors of 9-O-acetyl-GD2, but apparently in distinct compartments.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 190-193 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T2 190-193 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T3 212-215 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T4 212-215 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2
T5 799-802 https://glytoucan.org/Structures/Glycans/G61168WC denotes GM2
T6 799-802 https://glytoucan.org/Structures/Glycans/G79389NT denotes GM2
T7 803-806 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T8 803-806 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2
T9 872-875 https://glytoucan.org/Structures/Glycans/G56516VH denotes GM3
T10 872-875 https://glytoucan.org/Structures/Glycans/G91237TK denotes GM3
T11 880-883 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T12 880-883 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T13 912-915 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T14 912-915 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T15 966-969 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T16 966-969 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T17 1230-1233 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T18 1230-1233 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T19 1237-1240 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T20 1237-1240 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T21 1367-1370 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T22 1367-1370 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T23 1841-1847 https://glytoucan.org/Structures/Glycans/G81533KY denotes Neu5Ac
T24 1983-1986 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T25 1983-1986 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T26 2031-2037 https://glytoucan.org/Structures/Glycans/G81533KY denotes Neu5Ac
T27 2065-2068 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T28 2065-2068 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2
T29 2073-2076 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T30 2073-2076 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T31 2259-2262 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T32 2259-2262 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2
T33 2377-2380 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T34 2377-2380 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2
T35 2400-2403 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T36 2400-2403 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T37 2455-2458 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T38 2455-2458 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2
T39 2502-2505 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T40 2502-2505 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2
T41 2527-2541 https://glytoucan.org/Structures/Glycans/G74121RX denotes 9-O-acetyl-GD3
T42 2538-2541 https://glytoucan.org/Structures/Glycans/G81928IQ denotes GD3
T43 2538-2541 https://glytoucan.org/Structures/Glycans/G98544DH denotes GD3
T44 2546-2549 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T45 2546-2549 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2
T46 2582-2585 https://glytoucan.org/Structures/Glycans/G02657AK denotes GD2
T47 2582-2585 https://glytoucan.org/Structures/Glycans/G41759HA denotes GD2

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 2527-2541 http://www.glycoepitope.jp/epitopes/EP0064 denotes 9-O-acetyl-GD3

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 515-520 http://purl.obolibrary.org/obo/MAT_0000488 denotes later
T2 715-723 http://purl.obolibrary.org/obo/MAT_0000491 denotes proximal

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 116-124 Disease denotes melanoma http://purl.obolibrary.org/obo/MONDO_0005105
T2 136-144 Disease denotes melanoma http://purl.obolibrary.org/obo/MONDO_0005105
T3 602-610 Disease denotes melanoma http://purl.obolibrary.org/obo/MONDO_0005105
T4 1488-1496 Disease denotes melanoma http://purl.obolibrary.org/obo/MONDO_0005105

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 181-193 Glycan denotes O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX
T2 212-215 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T3 799-802 Glycan denotes GM2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT
T4 803-806 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T5 872-875 Glycan denotes GM3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK
T6 880-883 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T7 912-915 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T8 966-969 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T9 1221-1233 Glycan denotes O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX
T10 1237-1240 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T11 1367-1370 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T12 1841-1847 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T13 1983-1986 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T14 2031-2037 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T15 2046-2052 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T16 2065-2068 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T17 2073-2076 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T18 2165-2171 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T19 2259-2262 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T20 2377-2380 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T21 2391-2403 Glycan denotes O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX
T22 2455-2458 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T23 2502-2505 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T24 2529-2541 Glycan denotes O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX
T25 2546-2549 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T26 2582-2585 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 116-124 Phenotype denotes melanoma HP:0002861
T2 136-144 Phenotype denotes melanoma HP:0002861
T3 602-610 Phenotype denotes melanoma HP:0002861
T4 1488-1496 Phenotype denotes melanoma HP:0002861

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 181-193 Glycan denotes O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX
T2 212-215 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T3 799-802 Glycan denotes GM2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT
T4 803-806 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T5 872-875 Glycan denotes GM3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK
T6 880-883 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T7 912-915 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T8 966-969 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T9 1221-1233 Glycan denotes O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX
T10 1237-1240 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T11 1367-1370 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T12 1841-1847 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T13 1983-1986 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T14 2031-2037 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T15 2046-2052 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T16 2065-2068 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T17 2073-2076 Glycan denotes GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH
T18 2165-2171 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T19 2259-2262 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T20 2377-2380 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T21 2391-2403 Glycan denotes O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX
T22 2455-2458 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T23 2502-2505 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T24 2529-2541 Glycan denotes O-acetyl-GD3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX
T25 2546-2549 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK
T26 2582-2585 Glycan denotes GD2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK

GlyCosmos15-HP

Id Subject Object Predicate Lexical cue hp_id
T1 116-124 Phenotype denotes melanoma HP:0002861
T2 136-144 Phenotype denotes melanoma HP:0002861
T3 602-610 Phenotype denotes melanoma HP:0002861
T4 1488-1496 Phenotype denotes melanoma HP:0002861

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1405-1410 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794
T2 1458-1463 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 116-124 Disease denotes melanoma MONDO:0005105
T2 136-144 Disease denotes melanoma MONDO:0005105
T3 602-610 Disease denotes melanoma MONDO:0005105
T4 1488-1496 Disease denotes melanoma MONDO:0005105

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 110-115 Organism denotes human 9606
T2 596-601 Organism denotes human 9606

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-131 Sentence denotes Kinetic and spatial interrelationships between ganglioside glycosyltransferases and O-acetyltransferase(s) in human melanoma cells.
T2 132-258 Sentence denotes The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized.
T3 259-407 Sentence denotes However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure.
T4 408-617 Sentence denotes Here, we have studied the spatial and temporal interrelationships among the activities responsible for the later stages of ganglioside biosynthesis and those for O-acetylation in cultured human melanoma cells.
T5 618-767 Sentence denotes First, brefeldin A treatment was used to separate biosynthetic steps into compartments distal or proximal to the transport block imposed by the drug.
T6 768-884 Sentence denotes In keeping with prior reports, GM2/GD2 synthase was consistently rendered inaccessible to its acceptors GM3 and GD3.
T7 885-952 Sentence denotes In contrast, the effect on GD3 biosynthesis was cell line-specific.
T8 953-1042 Sentence denotes Synthesis of GD3 was nearly abrogated in two lines, while it accumulated in a third line.
T9 1043-1166 Sentence denotes This indicates that the spatial organization of ganglioside processing activities can vary even between similar cell lines.
T10 1167-1429 Sentence denotes However, in all cell lines studied, the ratio of 9(7)-O-acetyl-GD3 to GD3 was not changed by brefeldin A, indicating that the majority of ganglioside O-acetyltransferase activity is co-localized with GD3 biosynthetic activity in the same Golgi subcompartment(s).
T11 1430-1583 Sentence denotes As an alternative approach, Golgi-enriched fractions from melanoma cells were incubated with radiolabeled and nonlabeled nucleotide sugars or acetyl-CoA.
T12 1584-1819 Sentence denotes In these preparations, biosynthesis is dependent upon the co-localization of appropriate sugar nucleotide transporters, glycosyltransferases, and acceptors that are endogenously present within intact topologically correct compartments.
T13 1820-2012 Sentence denotes Incubations with CMP-Neu5Ac and acetyl-CoA corroborated the results with brefeldin A, co-localizing ganglioside O-acetyltransferase activity in compartments where GD3 biosynthesis takes place.
T14 2013-2131 Sentence denotes Analyses with CMP-Neu5Ac and UDP-GalNAc showed that GD2 and GD3 synthesis occur in partially overlapping compartments.
T15 2132-2381 Sentence denotes Labeling with acetyl-CoA and UDP-GalNAc indicated that although labeled acetate can be transferred from acetyl-CoA directly to GD2, ganglioside O-acetyltransferase activity does not substantially overlap with the biosynthetic compartment(s) for GD2.
T16 2382-2515 Sentence denotes Instead, O-acetyl-GD3 appears to be co-localized with the compartment of GD2 biosynthesis and serves as an acceptor for GD2 synthase.
T17 2516-2627 Sentence denotes Thus, both 9-O-acetyl-GD3 and GD2 can be precursors of 9-O-acetyl-GD2, but apparently in distinct compartments.

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 2527-2541 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes 9-O-acetyl-GD3 http://www.glycoepitope.jp/epitopes/EP0064

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 692-704 Body_part denotes compartments FMA:76577
T2 1806-1818 Body_part denotes compartments FMA:76577
T3 1964-1976 Body_part denotes compartments FMA:76577
T4 2118-2130 Body_part denotes compartments FMA:76577
T5 2358-2369 Body_part denotes compartment FMA:76577
T6 2440-2451 Body_part denotes compartment FMA:76577
T7 2614-2626 Body_part denotes compartments FMA:76577

GlyCosmos15-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 515-520 Body_part denotes later http://purl.obolibrary.org/obo/MAT_0000488
T2 715-723 Body_part denotes proximal http://purl.obolibrary.org/obo/MAT_0000491

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 110-115 OrganismTaxon denotes human 9606
T2 596-601 OrganismTaxon denotes human 9606

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 2527-2541 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes 9-O-acetyl-GD3 http://www.glycoepitope.jp/epitopes/EP0064

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1405-1410 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794
T2 1458-1463 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T2 715-723 Body_part denotes proximal http://purl.obolibrary.org/obo/MAT_0000491