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PubMed:8440713 JSONTXT

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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 809-816 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ

sentences

Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes Identification and purification of a calcium-binding protein in hepatic nuclear membranes.
T2 91-213 Sentence denotes Recent evidence suggests that nuclei possess Ca2+ transport mechanisms to regulate nucleoplasmic/cytosolic Ca2+ gradients.
T3 214-321 Sentence denotes We, therefore, investigated the possibility that Ca(2+)-binding proteins may also exist within the nucleus.
T4 322-689 Sentence denotes Electrophoretic analysis revealed the presence of an acidic 93-kDa protein (p93) in the membranes of isolated nuclei. p93 stained blue with "Stains-All" in SDS-polyacrylamide gels and was the major 45Ca(2+)- and ruthenium red-binding nuclear envelope protein in electroblot overlays. p93 was resistant to extraction by 6 M urea but was solubilized in 2% Triton X-100.
T5 690-853 Sentence denotes Citric acid was highly effective in removing the outer nuclear membrane (ER) with concomitant reduction (< 10-fold) of mannose-6-phosphatase activity, but not p93.
T6 854-981 Sentence denotes 45Ca(2+)-binding assays of purified p93 revealed the presence of high capacity Ca(2+)-binding sites comparable to calreticulin.
T7 982-1096 Sentence denotes This evidence strongly suggests that p93 is a major Ca(2+)-binding protein of the inner nuclear envelope membrane.
T8 1097-1252 Sentence denotes Partial amino acid sequence analysis revealed that p93 was close to 100% homologous with a recently identified ER Ca(2+)-binding protein known as calnexin.
T9 1253-1299 Sentence denotes It is likely, therefore, that p93 is calnexin.
T10 1300-1463 Sentence denotes However, mild CHAPS detergent treatment of nuclear envelopes and ER revealed distinctly different solubility properties of each membrane for the extraction of p93.
T11 1464-1608 Sentence denotes This, together with the citrate data, strongly suggests that p93/calnexin, in isolated nuclear envelopes, is mostly bound to the inner membrane.
T12 1609-1734 Sentence denotes It is possible that p93 may be involved with the regulation of Ca2+ transients between the nucleoplasm and perinuclear space.
T1 0-90 Sentence denotes Identification and purification of a calcium-binding protein in hepatic nuclear membranes.
T2 91-213 Sentence denotes Recent evidence suggests that nuclei possess Ca2+ transport mechanisms to regulate nucleoplasmic/cytosolic Ca2+ gradients.
T3 214-321 Sentence denotes We, therefore, investigated the possibility that Ca(2+)-binding proteins may also exist within the nucleus.
T4 322-689 Sentence denotes Electrophoretic analysis revealed the presence of an acidic 93-kDa protein (p93) in the membranes of isolated nuclei. p93 stained blue with "Stains-All" in SDS-polyacrylamide gels and was the major 45Ca(2+)- and ruthenium red-binding nuclear envelope protein in electroblot overlays. p93 was resistant to extraction by 6 M urea but was solubilized in 2% Triton X-100.
T5 690-853 Sentence denotes Citric acid was highly effective in removing the outer nuclear membrane (ER) with concomitant reduction (< 10-fold) of mannose-6-phosphatase activity, but not p93.
T6 854-981 Sentence denotes 45Ca(2+)-binding assays of purified p93 revealed the presence of high capacity Ca(2+)-binding sites comparable to calreticulin.
T7 982-1096 Sentence denotes This evidence strongly suggests that p93 is a major Ca(2+)-binding protein of the inner nuclear envelope membrane.
T8 1097-1252 Sentence denotes Partial amino acid sequence analysis revealed that p93 was close to 100% homologous with a recently identified ER Ca(2+)-binding protein known as calnexin.
T9 1253-1299 Sentence denotes It is likely, therefore, that p93 is calnexin.
T10 1300-1463 Sentence denotes However, mild CHAPS detergent treatment of nuclear envelopes and ER revealed distinctly different solubility properties of each membrane for the extraction of p93.
T11 1464-1608 Sentence denotes This, together with the citrate data, strongly suggests that p93/calnexin, in isolated nuclear envelopes, is mostly bound to the inner membrane.
T12 1609-1734 Sentence denotes It is possible that p93 may be involved with the regulation of Ca2+ transients between the nucleoplasm and perinuclear space.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 809-816 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 72-89 Body_part denotes nuclear membranes http://purl.obolibrary.org/obo/GO_0031965
T2 313-320 Body_part denotes nucleus http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125
T4 410-419 Body_part denotes membranes http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T7 556-572 Body_part denotes nuclear envelope http://purl.obolibrary.org/obo/GO_0005635
T8 745-761 Body_part denotes nuclear membrane http://purl.obolibrary.org/obo/GO_0031965
T9 1070-1086 Body_part denotes nuclear envelope http://purl.obolibrary.org/obo/GO_0005635
T10 1087-1095 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T13 1343-1360 Body_part denotes nuclear envelopes http://purl.obolibrary.org/obo/GO_0005635
T14 1428-1436 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T17 1551-1568 Body_part denotes nuclear envelopes http://purl.obolibrary.org/obo/GO_0005635
T18 1599-1607 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T21 1728-1733 Body_part denotes space http://purl.obolibrary.org/obo/UBERON_0000464