PubMed:8407896
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/8407896","sourcedb":"PubMed","sourceid":"8407896","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/8407896","text":"Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone.\nPrevious attempts to express glycosylphosphatidylinositol-anchored proteins in Ltk- cells have not been successful because Ltk- cells cannot synthesize N-acetylglucosamine-phosphatidylinositol, the first intermediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesis and allows the expression of glycosylphosphatidylinositol-anchored proteins in Ltk- cells. The nucleotide sequence predicts a novel cytosolic protein of 188 amino acids.","tracks":[{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":274,"end":293},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G64581RP"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":121},"obj":"Sentence"},{"id":"T2","span":{"begin":122,"end":362},"obj":"Sentence"},{"id":"T3","span":{"begin":363,"end":564},"obj":"Sentence"},{"id":"T4","span":{"begin":565,"end":643},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":121},"obj":"Sentence"},{"id":"T2","span":{"begin":122,"end":362},"obj":"Sentence"},{"id":"T3","span":{"begin":363,"end":564},"obj":"Sentence"},{"id":"T4","span":{"begin":565,"end":643},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":274,"end":293},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":104,"end":109},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":415,"end":420},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#ec93bf","default":true},{"id":"sentences","color":"#93d9ec"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#ece593"},{"id":"NCBITAXON","color":"#cb93ec"}]}]}}