PubMed:8399306 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/8399306","sourcedb":"PubMed","sourceid":"8399306","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/8399306","text":"The alpha-D-glucosyl C-2 hydroxyl is required for binding to the H(+)-sucrose transporter in phloem.\nThe specificity of uptake of sucrose into isolated phloem tissue from Apium graveolens has been investigated using a number of analogues of sucrose. The presence of a single saturable transport system for sucrose was confirmed using the double isotope method of Inui and Christensen (J. Gen. Physiol. 50 (1966) 203-224). 4-Hydroxyphenyl beta-D-fructofuranoside showed no inhibition of sucrose uptake, whereas 4-hydroxyphenyl alpha-D-glucopyranoside showed competitive inhibition with a Ki of 6.7 mM. 4-Methoxyphenyl alpha-D-glucopyranoside also inhibited sucrose uptake competitively (Ki = 6.0 mM). This compound was also synthesised radioactively labelled with 14C and its uptake into phloem tissue was conclusively demonstrated to occur by active transport on the same carrier as sucrose. Contrastingly, 4-methoxyphenyl alpha-D-2-deoxyglucopyranoside displayed non-competitive inhibition of sucrose influx (Ki = 2.5 mM) and uptake of the 14C-labelled compound was insensitive to FCCP, PCMBS and sucrose. We conclude that the hydroxyl group at the C-2 position on the glucopyranosyl moiety is essential for binding to the sucrose carrier in this tissue.","tracks":[{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":171,"end":187},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"4045"},{"subj":"T1","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":93,"end":99},"obj":"Body_part"},{"id":"T2","span":{"begin":152,"end":158},"obj":"Body_part"},{"id":"T3","span":{"begin":787,"end":793},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000364"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000364"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000364"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"},{"subj":"T2","pred":"source","obj":"Anatomy-MAT"},{"subj":"T3","pred":"source","obj":"Anatomy-MAT"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":159,"end":165},"obj":"Body_part"},{"id":"T2","span":{"begin":794,"end":800},"obj":"Body_part"},{"id":"T3","span":{"begin":1248,"end":1254},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":585,"end":589},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0001919"},{"subj":"T1","pred":"source","obj":"HP-phenotype"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"NCBITAXON","color":"#d2ec93","default":true},{"id":"Anatomy-MAT","color":"#ec93ec"},{"id":"Anatomy-UBERON","color":"#93ecd2"},{"id":"HP-phenotype","color":"#ecb893"}]}]}}