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PubMed:8397207 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-104 Sentence denotes Characterization of the in vitro reconstituted cyclin A or B1-dependent cdk2 and cdc2 kinase activities.
T2 105-233 Sentence denotes Human cyclins A and B1 were assembled with the cdk2 or cdc2 protein to reconstitute their respective kinase activities in vitro.
T3 234-357 Sentence denotes Both cyclins complemented either cdk2 or cdc2, yielding kinase activities that supported the phosphorylation of histone H1.
T4 358-681 Sentence denotes Activation of cdk2-catalyzed H1 kinase activity by cyclin A required a 10-min preincubation of the two components, whereas cdc2 kinase supported phosphate incorporation without a detectable time lag upon the addition of cyclin B1, suggesting a slower association rate of cdk2 with cyclin A compared with cdc2 and cyclin B1.
T5 682-859 Sentence denotes Both cdk2 and cyclin A, as well as cdc2 and cyclin B1, formed stable complexes in the absence of ATP and substrate that could be isolated after glycerol gradient centrifugation.
T6 860-968 Sentence denotes Incubation of the isolated complexes with ATP and histone H1 supported the phosphorylation of the substrate.
T7 969-1117 Sentence denotes Cyclin A-activated cdk2 or cdc2 phosphorylated p107, a pRB-related cellular protein, 10 times more effectively than the cyclin B1-complexed kinases.
T8 1118-1198 Sentence denotes This was most likely due to a direct association of cyclin A with p107 (Ewen, M.
T9 1199-1289 Sentence denotes E., Faha, B., Harlow, E., and Livingston, D. (1992) Science 255, 85-87; Faha, B., Ewen, M.
T10 1290-1365 Sentence denotes E., Tsai, L.-H., Livingston, D., and Harlow, E. (1992) Science 255, 87-90).
T11 1366-1628 Sentence denotes The reconstituted cdc2-cyclin B1 complex incorporated 4-5-fold more phosphate into the p34 subunit of the three-subunit (p70, p34, and p14) human single-stranded DNA-binding protein (also called RP-A), a DNA replication and DNA repair factor, than cdc2-cyclin A.
T12 1629-1738 Sentence denotes No detectable phosphorylation of the p34 protein was observed with cdk2 complexed with either cyclin B1 or A.
T13 1739-1897 Sentence denotes These data indicate that both cyclins as well as the catalytic subunits are important factors in controlling the rate of phosphorylation of a given substrate.
T14 1898-2029 Sentence denotes The cyclin-activated cdc2 family kinases may target their cellular substrates through cyclin-mediated protein-protein interactions.
T1 0-104 Sentence denotes Characterization of the in vitro reconstituted cyclin A or B1-dependent cdk2 and cdc2 kinase activities.
T2 105-233 Sentence denotes Human cyclins A and B1 were assembled with the cdk2 or cdc2 protein to reconstitute their respective kinase activities in vitro.
T3 234-357 Sentence denotes Both cyclins complemented either cdk2 or cdc2, yielding kinase activities that supported the phosphorylation of histone H1.
T4 358-681 Sentence denotes Activation of cdk2-catalyzed H1 kinase activity by cyclin A required a 10-min preincubation of the two components, whereas cdc2 kinase supported phosphate incorporation without a detectable time lag upon the addition of cyclin B1, suggesting a slower association rate of cdk2 with cyclin A compared with cdc2 and cyclin B1.
T5 682-859 Sentence denotes Both cdk2 and cyclin A, as well as cdc2 and cyclin B1, formed stable complexes in the absence of ATP and substrate that could be isolated after glycerol gradient centrifugation.
T6 860-968 Sentence denotes Incubation of the isolated complexes with ATP and histone H1 supported the phosphorylation of the substrate.
T7 969-1117 Sentence denotes Cyclin A-activated cdk2 or cdc2 phosphorylated p107, a pRB-related cellular protein, 10 times more effectively than the cyclin B1-complexed kinases.
T8 1118-1198 Sentence denotes This was most likely due to a direct association of cyclin A with p107 (Ewen, M.
T9 1199-1289 Sentence denotes E., Faha, B., Harlow, E., and Livingston, D. (1992) Science 255, 85-87; Faha, B., Ewen, M.
T10 1290-1365 Sentence denotes E., Tsai, L.-H., Livingston, D., and Harlow, E. (1992) Science 255, 87-90).
T11 1366-1628 Sentence denotes The reconstituted cdc2-cyclin B1 complex incorporated 4-5-fold more phosphate into the p34 subunit of the three-subunit (p70, p34, and p14) human single-stranded DNA-binding protein (also called RP-A), a DNA replication and DNA repair factor, than cdc2-cyclin A.
T12 1629-1738 Sentence denotes No detectable phosphorylation of the p34 protein was observed with cdk2 complexed with either cyclin B1 or A.
T13 1739-1897 Sentence denotes These data indicate that both cyclins as well as the catalytic subunits are important factors in controlling the rate of phosphorylation of a given substrate.
T14 1898-2029 Sentence denotes The cyclin-activated cdc2 family kinases may target their cellular substrates through cyclin-mediated protein-protein interactions.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 47-55 OrganismTaxon denotes cyclin A 120565
T2 105-110 OrganismTaxon denotes Human 9606
T3 409-417 OrganismTaxon denotes cyclin A 120565
T4 639-647 OrganismTaxon denotes cyclin A 120565
T5 696-704 OrganismTaxon denotes cyclin A 120565
T6 969-977 OrganismTaxon denotes Cyclin A 120565
T7 1170-1178 OrganismTaxon denotes cyclin A 120565
T8 1506-1511 OrganismTaxon denotes human 9606
T9 1619-1627 OrganismTaxon denotes cyclin A 120565