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PubMed:8397193 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-108 Sentence denotes Specific modification of structure and property of myoglobin by the formation of tetrazolylhistidine 64(E7).
T2 109-166 Sentence denotes Reaction of the modified myoglobin with molecular oxygen.
T3 167-426 Sentence denotes Tetrazole-myoglobin (Tet-Mb), a site selectively modified myoglobin with tetrazole anion (-CN4-) covalently attached to the imidazole N epsilon of the distal histidine 64(E7) (see Fig. 1; Kamiya, N., Shiro, Y., Iwata, T., Iizuka, T., and Iwasaki, H. (1991) J.
T4 427-430 Sentence denotes Am.
T5 431-436 Sentence denotes Chem.
T6 437-441 Sentence denotes Soc.
T7 442-580 Sentence denotes 113, 1826-1829), exhibited unique properties in the reduction from ferric to ferrous states and in the reaction of its deoxy form with O2.
T8 581-802 Sentence denotes The redox potential of Tet-Mb is obtained to be -193 mV, which is much lower than that of unmodified (native) myoglobin (50 mV), possibly due to the electrostatic interaction between the heme iron and the tetrazole group.
T9 803-1001 Sentence denotes The ferrous deoxy form of Tet-Mb was rapidly oxidized to its ferric form in the reaction with O2 at room temperature through an intermediary formation of its oxy form and with the generation of O2-.
T10 1002-1247 Sentence denotes The oxy form of Tet-Mb can be detected by the optical spectral measurement at -12 degrees C, the rapid scan measurement at room temperature, and the electron spin resonance measurement of its cobalt-substituted derivative (Tet-Mb(Co2+)) at 77 K.
T11 1248-1553 Sentence denotes In the kinetic measurement of the O2 binding reaction to Tet-Mb, its association and dissociation rate constants in the bimolecular reaction were 6.1 x 10(7) M-1 s-1 and 2200 s-1, respectively, showing that the tetrazole modification of His-64 extremely accelerates its association and dissociation rates.
T12 1554-1776 Sentence denotes Taken together with the extremely fast autoxidation rate (53 h-1) obtained, these kinetic results suggested that the channel of O2 from the solvent region to the protein interior is open enough to pass the external ligand.
T13 1777-1849 Sentence denotes The structure is discussed in relation to those of some genetic mutants.
T14 1850-2057 Sentence denotes Taking these properties, we demonstrated that Tet-Mb can catalyze O2 consumption to generate O2-, coupled with the NADH-supported enzymatic reduction system of cytochrome P-450cam under an aerobic condition.
T1 0-108 Sentence denotes Specific modification of structure and property of myoglobin by the formation of tetrazolylhistidine 64(E7).
T2 109-166 Sentence denotes Reaction of the modified myoglobin with molecular oxygen.
T3 167-426 Sentence denotes Tetrazole-myoglobin (Tet-Mb), a site selectively modified myoglobin with tetrazole anion (-CN4-) covalently attached to the imidazole N epsilon of the distal histidine 64(E7) (see Fig. 1; Kamiya, N., Shiro, Y., Iwata, T., Iizuka, T., and Iwasaki, H. (1991) J.
T4 427-430 Sentence denotes Am.
T5 431-436 Sentence denotes Chem.
T6 437-441 Sentence denotes Soc.
T7 442-580 Sentence denotes 113, 1826-1829), exhibited unique properties in the reduction from ferric to ferrous states and in the reaction of its deoxy form with O2.
T8 581-802 Sentence denotes The redox potential of Tet-Mb is obtained to be -193 mV, which is much lower than that of unmodified (native) myoglobin (50 mV), possibly due to the electrostatic interaction between the heme iron and the tetrazole group.
T9 803-1001 Sentence denotes The ferrous deoxy form of Tet-Mb was rapidly oxidized to its ferric form in the reaction with O2 at room temperature through an intermediary formation of its oxy form and with the generation of O2-.
T10 1002-1247 Sentence denotes The oxy form of Tet-Mb can be detected by the optical spectral measurement at -12 degrees C, the rapid scan measurement at room temperature, and the electron spin resonance measurement of its cobalt-substituted derivative (Tet-Mb(Co2+)) at 77 K.
T11 1248-1553 Sentence denotes In the kinetic measurement of the O2 binding reaction to Tet-Mb, its association and dissociation rate constants in the bimolecular reaction were 6.1 x 10(7) M-1 s-1 and 2200 s-1, respectively, showing that the tetrazole modification of His-64 extremely accelerates its association and dissociation rates.
T12 1554-1776 Sentence denotes Taken together with the extremely fast autoxidation rate (53 h-1) obtained, these kinetic results suggested that the channel of O2 from the solvent region to the protein interior is open enough to pass the external ligand.
T13 1777-1849 Sentence denotes The structure is discussed in relation to those of some genetic mutants.
T14 1850-2057 Sentence denotes Taking these properties, we demonstrated that Tet-Mb can catalyze O2 consumption to generate O2-, coupled with the NADH-supported enzymatic reduction system of cytochrome P-450cam under an aerobic condition.

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 303-310 Cell denotes epsilon http://purl.obolibrary.org/obo/CL:0003025