> top > docs > PubMed:8394350 > annotations

PubMed:8394350 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
T1 0-113 Sentence denotes Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
T2 114-212 Sentence denotes A protein phosphatase (PPase) from the bacteriophage lambda was overexpressed in Escherichia coli.
T3 213-344 Sentence denotes The recombinant enzyme was purified to homogeneity yielding approximately 17 mg of enzyme from a single liter of bacterial culture.
T4 345-441 Sentence denotes Biochemical characterization of the enzyme showed that it required Mn2+ or Ni2+ as an activator.
T5 442-551 Sentence denotes The recombinant enzyme was active toward serine, threonine, and tyrosine phosphoproteins and phosphopeptides.
T6 552-657 Sentence denotes Surprisingly, the bacterial histidyl phosphoprotein, NRII, was also dephosphorylated by the lambda-PPase.
T7 658-861 Sentence denotes The lambda-PPase shares a number of kinetic and structural properties with the eukaryotic Ser/Thr phosphatases, suggesting that the lambda-PPase will serve as a good model for structure-function studies.
T8 862-947 Sentence denotes Crystallization of the recombinant purified lambda-PPase yielded monoclinic crystals.
T9 948-1023 Sentence denotes The crystals diffract to 4.0 A when exposed to synchrotron x-ray radiation.
T1 0-113 Sentence denotes Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
T2 114-212 Sentence denotes A protein phosphatase (PPase) from the bacteriophage lambda was overexpressed in Escherichia coli.
T3 213-344 Sentence denotes The recombinant enzyme was purified to homogeneity yielding approximately 17 mg of enzyme from a single liter of bacterial culture.
T4 345-441 Sentence denotes Biochemical characterization of the enzyme showed that it required Mn2+ or Ni2+ as an activator.
T5 442-551 Sentence denotes The recombinant enzyme was active toward serine, threonine, and tyrosine phosphoproteins and phosphopeptides.
T6 552-657 Sentence denotes Surprisingly, the bacterial histidyl phosphoprotein, NRII, was also dephosphorylated by the lambda-PPase.
T7 658-861 Sentence denotes The lambda-PPase shares a number of kinetic and structural properties with the eukaryotic Ser/Thr phosphatases, suggesting that the lambda-PPase will serve as a good model for structure-function studies.
T8 862-947 Sentence denotes Crystallization of the recombinant purified lambda-PPase yielded monoclinic crystals.
T9 948-1023 Sentence denotes The crystals diffract to 4.0 A when exposed to synchrotron x-ray radiation.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 195-211 OrganismTaxon denotes Escherichia coli 562

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 167-173 Body_part denotes lambda http://purl.obolibrary.org/obo/UBERON_0013424
T2 644-650 Body_part denotes lambda http://purl.obolibrary.org/obo/UBERON_0013424
T3 662-668 Body_part denotes lambda http://purl.obolibrary.org/obo/UBERON_0013424
T4 790-796 Body_part denotes lambda http://purl.obolibrary.org/obo/UBERON_0013424
T5 906-912 Body_part denotes lambda http://purl.obolibrary.org/obo/UBERON_0013424