| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-186 |
Sentence |
denotes |
Competition between transcription factors HNF1 and HNF3, and alternative cell-specific activation by DBP and C/EBP contribute to the regulation of the liver-specific aldolase B promoter. |
| TextSentencer_T2 |
187-338 |
Sentence |
denotes |
The aldolase B proximal promoter is controlled by at least five elements spanning from -190 to -103 bp with respect to the start site of transcription. |
| TextSentencer_T3 |
339-575 |
Sentence |
denotes |
From 5' to 3', we found: a negative DE element, an activating C/EBP-DBP binding site, a CCAAT box binding NFY that seems to play a negative role, and an activating element consisting of two overlapping binding sites for HNF-1 and HNF-3. |
| TextSentencer_T4 |
576-743 |
Sentence |
denotes |
Contransfection experiments of aldolase B/CAT constructs and of expression vectors for different transcription factors were carried out in human hepatoma Hep G2 cells. |
| TextSentencer_T5 |
744-920 |
Sentence |
denotes |
We found that DBP and HNF-1 are strong transactivators of the aldolase B promoter while C/EBP and vHNF-1 are only weak activators and HNF-3 alone does not modify such activity. |
| TextSentencer_T6 |
921-1067 |
Sentence |
denotes |
Deletion of the distal negative element results in a similar transactivation by C/EBP and DBP, enhanced for the former and reduced for the latter. |
| TextSentencer_T7 |
1068-1172 |
Sentence |
denotes |
In hepatocytes in primary culture, the strong transactivator is C/EBP while DBP is essentially inactive. |
| TextSentencer_T8 |
1173-1321 |
Sentence |
denotes |
This tissue-specificity of C/EBP and DBP action could depend on interaction with tissue-specific proteins bound to a neighbouring site, probably DE. |
| TextSentencer_T9 |
1322-1403 |
Sentence |
denotes |
Finally, HNF3 behaves as a very strong anti-activator of the aldolase B promoter. |
| TextSentencer_T10 |
1404-1503 |
Sentence |
denotes |
It competitively antagonizes transactivation by HNF-1 and non-competitively transactivation by DBP. |
| TextSentencer_T11 |
1504-1654 |
Sentence |
denotes |
This negative effect of HNF-3 and tissue-specificity of the transactivation potential of DBP and C/EBP are unique features of the aldolase B promoter. |
| T1 |
0-186 |
Sentence |
denotes |
Competition between transcription factors HNF1 and HNF3, and alternative cell-specific activation by DBP and C/EBP contribute to the regulation of the liver-specific aldolase B promoter. |
| T2 |
187-338 |
Sentence |
denotes |
The aldolase B proximal promoter is controlled by at least five elements spanning from -190 to -103 bp with respect to the start site of transcription. |
| T3 |
339-575 |
Sentence |
denotes |
From 5' to 3', we found: a negative DE element, an activating C/EBP-DBP binding site, a CCAAT box binding NFY that seems to play a negative role, and an activating element consisting of two overlapping binding sites for HNF-1 and HNF-3. |
| T4 |
576-743 |
Sentence |
denotes |
Contransfection experiments of aldolase B/CAT constructs and of expression vectors for different transcription factors were carried out in human hepatoma Hep G2 cells. |
| T5 |
744-920 |
Sentence |
denotes |
We found that DBP and HNF-1 are strong transactivators of the aldolase B promoter while C/EBP and vHNF-1 are only weak activators and HNF-3 alone does not modify such activity. |
| T6 |
921-1067 |
Sentence |
denotes |
Deletion of the distal negative element results in a similar transactivation by C/EBP and DBP, enhanced for the former and reduced for the latter. |
| T7 |
1068-1172 |
Sentence |
denotes |
In hepatocytes in primary culture, the strong transactivator is C/EBP while DBP is essentially inactive. |
| T8 |
1173-1321 |
Sentence |
denotes |
This tissue-specificity of C/EBP and DBP action could depend on interaction with tissue-specific proteins bound to a neighbouring site, probably DE. |
| T9 |
1322-1403 |
Sentence |
denotes |
Finally, HNF3 behaves as a very strong anti-activator of the aldolase B promoter. |
| T10 |
1404-1503 |
Sentence |
denotes |
It competitively antagonizes transactivation by HNF-1 and non-competitively transactivation by DBP. |
| T11 |
1504-1654 |
Sentence |
denotes |
This negative effect of HNF-3 and tissue-specificity of the transactivation potential of DBP and C/EBP are unique features of the aldolase B promoter. |