PubMed:8360477 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/8360477","sourcedb":"PubMed","sourceid":"8360477","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/8360477","text":"Involvement of IL-6 signal transducer gp130 in IL-11-mediated signal transduction.\nIL-11 is a novel cytokine with a variety of biofunctions which overlap with those of IL-6. We have previously identified IL-11 specific binding protein which is distinct from that of IL-6 in a number of cell lines. The similarities in biofunctions and differences in ligand binding proteins between IL-11 and IL-6 prompted us to investigate whether IL-11 shares common signal transduction mechanisms with IL-6. We have examined early signals triggered by IL-11 or IL-6 in a multifactor-dependent human erythroleukemic cell line, TF-1. The results showed that IL-11 and IL-6 can both stimulate cell proliferation, induce similar pattern of protein tyrosine phosphorylation, and activate the same proto-oncogene (junB) expression in TF-1 cells. These findings imply that IL-11 and IL-6 share similar early signaling events with the possibility of using the same signal transducer, gp130. We next tested whether IL-11 induced signaling can be inhibited by anti-gp130 antibodies which blocked IL-6-mediated functions. It was observed that anti-gp130 antibodies abolished cell proliferation, protein tyrosine phosphorylation, and junB gene expression elicited by IL-11 or IL-6 in TF-1 cells. The same antibodies, however, had no effect on granulocyte-macrophage colony stimulating factor or erythropoietin-induced TF-1 cell proliferation. Finally, anti-IL-6R antibody inhibited the ability of IL-6, but not IL-11, to transduce early signals in TF-1 cells. These results demonstrate that IL-11 and IL-6 utilize different ligand binding proteins, but share common signal transducer, gp130, in TF-1 cells.","tracks":[{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":15,"end":19},"obj":"protein"},{"id":"T2","span":{"begin":38,"end":43},"obj":"protein"},{"id":"T3","span":{"begin":47,"end":52},"obj":"protein"},{"id":"T4","span":{"begin":83,"end":88},"obj":"protein"},{"id":"T5","span":{"begin":168,"end":172},"obj":"protein"},{"id":"T6","span":{"begin":204,"end":209},"obj":"protein"},{"id":"T7","span":{"begin":266,"end":270},"obj":"protein"},{"id":"T8","span":{"begin":382,"end":387},"obj":"protein"},{"id":"T9","span":{"begin":392,"end":396},"obj":"protein"},{"id":"T10","span":{"begin":432,"end":437},"obj":"protein"},{"id":"T11","span":{"begin":488,"end":492},"obj":"protein"},{"id":"T12","span":{"begin":538,"end":543},"obj":"protein"},{"id":"T13","span":{"begin":547,"end":551},"obj":"protein"},{"id":"T14","span":{"begin":642,"end":647},"obj":"protein"},{"id":"T15","span":{"begin":652,"end":656},"obj":"protein"},{"id":"T16","span":{"begin":794,"end":798},"obj":"protein"},{"id":"T17","span":{"begin":852,"end":857},"obj":"protein"},{"id":"T18","span":{"begin":862,"end":866},"obj":"protein"},{"id":"T19","span":{"begin":962,"end":967},"obj":"protein"},{"id":"T20","span":{"begin":992,"end":997},"obj":"protein"},{"id":"T21","span":{"begin":1041,"end":1046},"obj":"protein"},{"id":"T22","span":{"begin":1072,"end":1076},"obj":"protein"},{"id":"T23","span":{"begin":1123,"end":1128},"obj":"protein"},{"id":"T24","span":{"begin":1208,"end":1212},"obj":"protein"},{"id":"T25","span":{"begin":1241,"end":1246},"obj":"protein"},{"id":"T26","span":{"begin":1250,"end":1254},"obj":"protein"},{"id":"T27","span":{"begin":1431,"end":1436},"obj":"protein"},{"id":"T28","span":{"begin":1471,"end":1475},"obj":"protein"},{"id":"T29","span":{"begin":1485,"end":1490},"obj":"protein"},{"id":"T30","span":{"begin":1565,"end":1570},"obj":"protein"},{"id":"T31","span":{"begin":1575,"end":1579},"obj":"protein"},{"id":"T32","span":{"begin":1659,"end":1664},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"AIMed"},{"subj":"T2","pred":"source","obj":"AIMed"},{"subj":"T3","pred":"source","obj":"AIMed"},{"subj":"T4","pred":"source","obj":"AIMed"},{"subj":"T5","pred":"source","obj":"AIMed"},{"subj":"T6","pred":"source","obj":"AIMed"},{"subj":"T7","pred":"source","obj":"AIMed"},{"subj":"T8","pred":"source","obj":"AIMed"},{"subj":"T9","pred":"source","obj":"AIMed"},{"subj":"T10","pred":"source","obj":"AIMed"},{"subj":"T11","pred":"source","obj":"AIMed"},{"subj":"T12","pred":"source","obj":"AIMed"},{"subj":"T13","pred":"source","obj":"AIMed"},{"subj":"T14","pred":"source","obj":"AIMed"},{"subj":"T15","pred":"source","obj":"AIMed"},{"subj":"T16","pred":"source","obj":"AIMed"},{"subj":"T17","pred":"source","obj":"AIMed"},{"subj":"T18","pred":"source","obj":"AIMed"},{"subj":"T19","pred":"source","obj":"AIMed"},{"subj":"T20","pred":"source","obj":"AIMed"},{"subj":"T21","pred":"source","obj":"AIMed"},{"subj":"T22","pred":"source","obj":"AIMed"},{"subj":"T23","pred":"source","obj":"AIMed"},{"subj":"T24","pred":"source","obj":"AIMed"},{"subj":"T25","pred":"source","obj":"AIMed"},{"subj":"T26","pred":"source","obj":"AIMed"},{"subj":"T27","pred":"source","obj":"AIMed"},{"subj":"T28","pred":"source","obj":"AIMed"},{"subj":"T29","pred":"source","obj":"AIMed"},{"subj":"T30","pred":"source","obj":"AIMed"},{"subj":"T31","pred":"source","obj":"AIMed"},{"subj":"T32","pred":"source","obj":"AIMed"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"AIMed","color":"#93b9ec","default":true}]}]}}