PubMed:8334704 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/8334704","sourcedb":"PubMed","sourceid":"8334704","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/8334704","text":"Mammalian Ras interacts directly with the serine/threonine kinase Raf.\nWe have identified proteins that interact with H-Ras using a two hybrid system screen of a mouse cDNA library. Approximately 50% of the clones identified encoded portions of the c-Raf and A-Raf serine/threonine kinases. Overlaps among these clones define a conserved 81 residue region of the N-terminus of Raf as the Ras interaction region. We show that Raf interacts with wild-type and activated Ras, but not with an effector domain mutant of Ras or with a dominant-interfering Ras mutant. Using purified bacterially expressed fusion proteins, we show, furthermore, that Ras and the N-terminal region of Raf associate directly in vitro and that this interaction is dependent on GTP bound to Ras.","tracks":[{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":162,"end":167},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10088"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10090"},{"subj":"T1","pred":"source","obj":"NCBITAXON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"NCBITAXON","color":"#93ec93","default":true}]}]}}