PubMed:8333853 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/8333853","sourcedb":"PubMed","sourceid":"8333853","text":"Cloning and expression of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase.\nHere we report the cDNA sequence of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase. The cDNA was isolated from a human placental cDNA library by screening with a 150bp probe generated by PCR using degenerate primers based on the sequences found in the sialyl motif. Comparative analysis of this cDNA with the rat liver alpha 2,3-sialyltransferase sequence indicates 91% nucleotide similarity between the two sequence in the predicted coding region. On the amino acid level, the degree of conservation is 97%. Surprisingly, Northern analysis indicated that the gene is expressed at low levels in human placenta but is abundantly expressed in skeletal muscle and fetal tissues.","tracks":[{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":47,"end":53},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":140,"end":146},"obj":"chem:24139"},{"id":"T1","span":{"begin":47,"end":53},"obj":"chem:24139"},{"id":"T2","span":{"begin":140,"end":146},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"attributes":[{"subj":"PD-NGLY1-deficiency-B_T1","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T2","pred":"source","obj":"NGLY1-deficiency"},{"subj":"T1","pred":"source","obj":"NGLY1-deficiency"},{"subj":"T2","pred":"source","obj":"NGLY1-deficiency"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"NGLY1-deficiency","color":"#93a2ec","default":true}]}]}}