Id |
Subject |
Object |
Predicate |
Lexical cue |
TextSentencer_T1 |
0-140 |
Sentence |
denotes |
Characterization of an arginine 789 to cysteine substitution in alpha 1 (II) collagen chains of a patient with spondyloepiphyseal dysplasia. |
TextSentencer_T2 |
141-312 |
Sentence |
denotes |
A child with spondyloepiphyseal dysplasia congenita was shown to be heterozygous for a mutation of the COL2A1 gene that encodes the alpha 1 (II) chain of type II collagen. |
TextSentencer_T3 |
313-430 |
Sentence |
denotes |
The alpha 1 (II) chains extracted from cartilage contained disulfide-bonded dimeric and trimeric alpha 1 (II) chains. |
TextSentencer_T4 |
431-582 |
Sentence |
denotes |
Carboxymethylation confirmed that some of the type II collagen chains contained cysteine residues that are not normally present in alpha 1 (II) chains. |
TextSentencer_T5 |
583-705 |
Sentence |
denotes |
Cyanogen bromide peptide mapping showed that the abnormal cysteine residue was located in the alpha 1 (II) CB10.5 peptide. |
TextSentencer_T6 |
706-935 |
Sentence |
denotes |
Amplification products of the corresponding region of alpha 1 (II) cDNA prepared from cultured dermal fibroblasts were shown by chemical cleavage and single strand conformation polymorphism analyses to contain a sequence anomaly. |
TextSentencer_T7 |
936-1109 |
Sentence |
denotes |
DNA sequencing showed a transition of C2913T in exon 41 of one allele of the COL2A1 gene resulting in the substitution of arginine 789 by cysteine in the alpha 1 (II) chain. |
TextSentencer_T8 |
1110-1301 |
Sentence |
denotes |
The mutation resulted in the loss of a MaeII cleavage site that was used to confirm that the proband was heterozygous for the mutation and that neither parent showed evidence of the mutation. |
TextSentencer_T9 |
1302-1428 |
Sentence |
denotes |
The type II collagen extracted from cartilage and from chondrocytes cultured in alginate beads showed similar characteristics. |
TextSentencer_T10 |
1429-1564 |
Sentence |
denotes |
Approximately a third of the type II collagen chains were mutant, and the secretion of molecules containing mutant chains was impaired. |
TextSentencer_T11 |
1565-1639 |
Sentence |
denotes |
The thermal stability of the collagen extracted from cartilage was normal. |
TextSentencer_T12 |
1640-1792 |
Sentence |
denotes |
This study confirmed the importance of dominant negative mutations of the COL2A1 gene in producing the spondyloepiphyseal dysplasia congenita phenotype. |
T1 |
0-140 |
Sentence |
denotes |
Characterization of an arginine 789 to cysteine substitution in alpha 1 (II) collagen chains of a patient with spondyloepiphyseal dysplasia. |
T2 |
141-312 |
Sentence |
denotes |
A child with spondyloepiphyseal dysplasia congenita was shown to be heterozygous for a mutation of the COL2A1 gene that encodes the alpha 1 (II) chain of type II collagen. |
T3 |
313-430 |
Sentence |
denotes |
The alpha 1 (II) chains extracted from cartilage contained disulfide-bonded dimeric and trimeric alpha 1 (II) chains. |
T4 |
431-582 |
Sentence |
denotes |
Carboxymethylation confirmed that some of the type II collagen chains contained cysteine residues that are not normally present in alpha 1 (II) chains. |
T5 |
583-705 |
Sentence |
denotes |
Cyanogen bromide peptide mapping showed that the abnormal cysteine residue was located in the alpha 1 (II) CB10.5 peptide. |
T6 |
706-935 |
Sentence |
denotes |
Amplification products of the corresponding region of alpha 1 (II) cDNA prepared from cultured dermal fibroblasts were shown by chemical cleavage and single strand conformation polymorphism analyses to contain a sequence anomaly. |
T7 |
936-1109 |
Sentence |
denotes |
DNA sequencing showed a transition of C2913T in exon 41 of one allele of the COL2A1 gene resulting in the substitution of arginine 789 by cysteine in the alpha 1 (II) chain. |
T8 |
1110-1301 |
Sentence |
denotes |
The mutation resulted in the loss of a MaeII cleavage site that was used to confirm that the proband was heterozygous for the mutation and that neither parent showed evidence of the mutation. |
T9 |
1302-1428 |
Sentence |
denotes |
The type II collagen extracted from cartilage and from chondrocytes cultured in alginate beads showed similar characteristics. |
T10 |
1429-1564 |
Sentence |
denotes |
Approximately a third of the type II collagen chains were mutant, and the secretion of molecules containing mutant chains was impaired. |
T11 |
1565-1639 |
Sentence |
denotes |
The thermal stability of the collagen extracted from cartilage was normal. |
T12 |
1640-1792 |
Sentence |
denotes |
This study confirmed the importance of dominant negative mutations of the COL2A1 gene in producing the spondyloepiphyseal dysplasia congenita phenotype. |