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Id Subject Object Predicate Lexical cue
T1 0-144 Sentence denotes The source and characteristics of chemiluminescence associated with the oxygenase reaction catalyzed by Mn(2+)-ribulosebisphosphate carboxylase.
T2 145-224 Sentence denotes We confirm the observation of Mogel and McFadden (Mogel, S.N., and McFadden, B.
T3 225-412 Sentence denotes A. (1990) Biochemistry 29, 8333-8337) that ribulosebisphosphate carboxylase/oxygenase (rubisco) exhibits chemiluminescence while catalyzing its oxygenase reaction in the presence of Mn2+.
T4 413-527 Sentence denotes However, our results with the spinach and Rhodospirillum rubrum enzymes differ markedly in the following respects.
T5 528-672 Sentence denotes 1) Chemiluminescence intensity was directly proportional to enzyme concentration and behaved as if representing the rate of oxygenase catalysis.
T6 673-750 Sentence denotes 2) The wavelength spectrum peaked at about 770 nm and extended beyond 810 nm.
T7 751-863 Sentence denotes This seems inconsistent with chemiluminescence generated by simultaneous decay of pairs of singlet O2 molecules.
T8 864-949 Sentence denotes It is consistent with manganese(II) luminescence and we discuss its possible sources.
T9 950-1388 Sentence denotes The time course of chemiluminescence (resolution, 0.25 s) was distinctively different for spinach and R. rubrum enzymes during the initial 5 s of catalysis, with the bacterial enzyme exhibiting a pronounced initial "burst." Chemiluminescence by the spinach enzyme responded to substrate concentrations in a manner consistent with known oxygenase properties, exhibiting Michaelis-Menten kinetics with ribulose-1,5-bisphosphate (Km 400 nM).
T10 1389-1507 Sentence denotes Chemiluminescence required carbamylated enzyme with Mn2+ bound at the active site (activation energy, -57.1 KJ.mol-1).
T11 1508-1686 Sentence denotes As an indicator of oxygenase activity, chemiluminescence represents an improvement over oxygen electrode measurements in response time and sensitivity by factors of at least 100.
T1 0-144 Sentence denotes The source and characteristics of chemiluminescence associated with the oxygenase reaction catalyzed by Mn(2+)-ribulosebisphosphate carboxylase.
T2 145-224 Sentence denotes We confirm the observation of Mogel and McFadden (Mogel, S.N., and McFadden, B.
T3 225-412 Sentence denotes A. (1990) Biochemistry 29, 8333-8337) that ribulosebisphosphate carboxylase/oxygenase (rubisco) exhibits chemiluminescence while catalyzing its oxygenase reaction in the presence of Mn2+.
T4 413-527 Sentence denotes However, our results with the spinach and Rhodospirillum rubrum enzymes differ markedly in the following respects.
T5 528-672 Sentence denotes 1) Chemiluminescence intensity was directly proportional to enzyme concentration and behaved as if representing the rate of oxygenase catalysis.
T6 673-750 Sentence denotes 2) The wavelength spectrum peaked at about 770 nm and extended beyond 810 nm.
T7 751-863 Sentence denotes This seems inconsistent with chemiluminescence generated by simultaneous decay of pairs of singlet O2 molecules.
T8 864-949 Sentence denotes It is consistent with manganese(II) luminescence and we discuss its possible sources.
T9 950-1388 Sentence denotes The time course of chemiluminescence (resolution, 0.25 s) was distinctively different for spinach and R. rubrum enzymes during the initial 5 s of catalysis, with the bacterial enzyme exhibiting a pronounced initial "burst." Chemiluminescence by the spinach enzyme responded to substrate concentrations in a manner consistent with known oxygenase properties, exhibiting Michaelis-Menten kinetics with ribulose-1,5-bisphosphate (Km 400 nM).
T10 1389-1507 Sentence denotes Chemiluminescence required carbamylated enzyme with Mn2+ bound at the active site (activation energy, -57.1 KJ.mol-1).
T11 1508-1686 Sentence denotes As an indicator of oxygenase activity, chemiluminescence represents an improvement over oxygen electrode measurements in response time and sensitivity by factors of at least 100.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 455-476 OrganismTaxon denotes Rhodospirillum rubrum 1085