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sentences

Id Subject Object Predicate Lexical cue
T1 0-74 Sentence denotes Nuclear localization of glycosaminoglycans in rat ovarian granulosa cells.
T2 75-278 Sentence denotes In rat ovarian granulosa cell cultures, internalized cell surface heparan sulfate is processed by either a fast lysosome-mediated pathway or by a slow pathway which generates glycosaminoglycan fragments.
T3 279-476 Sentence denotes Cell-associated dermatan sulfate proteoglycans also undergo processing through analogous pathways, although the slow pathway does not involve endoglycosidic cleavage of the dermatan sulfate chains.
T4 477-628 Sentence denotes In the present study we tested whether intracellular glycosaminoglycan fragments in rat ovarian granulosa cells were transient residents of the nuclei.
T5 629-759 Sentence denotes A technique for isolating nuclei was devised in which cells were lysed with a hypo-osmotic extraction buffer containing detergent.
T6 760-898 Sentence denotes Nuclei were then purified by conventional methods, and final preparations gave excellent recovery of the starting DNA (approximately 90%).
T7 899-1026 Sentence denotes The technique was used to isolate glycosaminoglycans from nuclei after cells were metabolically radiolabeled with [35S]sulfate.
T8 1027-1153 Sentence denotes The results indicated the possible presence of dermatan sulfate, but not heparan sulfate, glycosaminoglycans at this location.
T9 1154-1341 Sentence denotes Failure to remove cell surface proteoglycans with trypsin before preparation of nuclei resulted in nuclear contamination with significant amounts of intact dermatan sulfate proteoglycans.
T10 1342-1549 Sentence denotes Nuclei preparations obtained using traditional homogenization steps from cells either treated without or with trypsin gave identical results except that recoveries of DNA were much lower (approximately 30%).
T11 1550-1713 Sentence denotes The results demonstrate the difficulty in isolating pure nuclei and therefore also of firm conclusions pertaining to the nuclear association of glycosaminoglycans.
T1 0-74 Sentence denotes Nuclear localization of glycosaminoglycans in rat ovarian granulosa cells.
T2 75-278 Sentence denotes In rat ovarian granulosa cell cultures, internalized cell surface heparan sulfate is processed by either a fast lysosome-mediated pathway or by a slow pathway which generates glycosaminoglycan fragments.
T3 279-476 Sentence denotes Cell-associated dermatan sulfate proteoglycans also undergo processing through analogous pathways, although the slow pathway does not involve endoglycosidic cleavage of the dermatan sulfate chains.
T4 477-628 Sentence denotes In the present study we tested whether intracellular glycosaminoglycan fragments in rat ovarian granulosa cells were transient residents of the nuclei.
T5 629-759 Sentence denotes A technique for isolating nuclei was devised in which cells were lysed with a hypo-osmotic extraction buffer containing detergent.
T6 760-898 Sentence denotes Nuclei were then purified by conventional methods, and final preparations gave excellent recovery of the starting DNA (approximately 90%).
T7 899-1026 Sentence denotes The technique was used to isolate glycosaminoglycans from nuclei after cells were metabolically radiolabeled with [35S]sulfate.
T8 1027-1153 Sentence denotes The results indicated the possible presence of dermatan sulfate, but not heparan sulfate, glycosaminoglycans at this location.
T9 1154-1341 Sentence denotes Failure to remove cell surface proteoglycans with trypsin before preparation of nuclei resulted in nuclear contamination with significant amounts of intact dermatan sulfate proteoglycans.
T10 1342-1549 Sentence denotes Nuclei preparations obtained using traditional homogenization steps from cells either treated without or with trypsin gave identical results except that recoveries of DNA were much lower (approximately 30%).
T11 1550-1713 Sentence denotes The results demonstrate the difficulty in isolating pure nuclei and therefore also of firm conclusions pertaining to the nuclear association of glycosaminoglycans.

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 141-156 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate
T2 1100-1115 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 295-325 Disease denotes dermatan sulfate proteoglycans http://purl.obolibrary.org/obo/MONDO_0007526|http://purl.obolibrary.org/obo/MONDO_0020682
T3 1310-1340 Disease denotes dermatan sulfate proteoglycans http://purl.obolibrary.org/obo/MONDO_0007526|http://purl.obolibrary.org/obo/MONDO_0020682

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 46-49 OrganismTaxon denotes rat 10114|10116
T3 78-81 OrganismTaxon denotes rat 10114|10116
T5 561-564 OrganismTaxon denotes rat 10114|10116

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 58-73 Body_part denotes granulosa cells http://purl.obolibrary.org/obo/CL_0000501
T2 90-104 Body_part denotes granulosa cell http://purl.obolibrary.org/obo/CL_0000501
T3 187-195 Body_part denotes lysosome http://purl.obolibrary.org/obo/GO_0005764
T4 516-529 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T5 573-588 Body_part denotes granulosa cells http://purl.obolibrary.org/obo/CL_0000501

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 58-73 Cell denotes granulosa cells http://purl.obolibrary.org/obo/CL:0000501
T2 90-104 Cell denotes granulosa cell http://purl.obolibrary.org/obo/CL:0000501
T3 573-588 Cell denotes granulosa cells http://purl.obolibrary.org/obo/CL:0000501