PubMed:8292036 JSONTXT

{"project":"ggdb-test","denotations":[{"id":"T1","span":{"begin":123,"end":128},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"T2","span":{"begin":315,"end":320},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"T3","span":{"begin":427,"end":432},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"T4","span":{"begin":513,"end":518},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"T5","span":{"begin":747,"end":752},"obj":"https://acgg.asia/db/ggdb/info/gg130"}],"text":"cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.\nHuman N-acetylglucosaminyltransferase V (GnT-V, EC 2.4.1.155) cDNA was isolated from a human fetal liver cDNA library. Oligonucleotide primers for polymerase chain reaction were designed according to the amino acid sequence of human GnT-V. Screening for the cDNA was carried out by plaque hybridization using PCR products of about 500 bp. Human GnT-V has 741 amino acids and six putative N-glycosylation sites. The homology to rat GnT-V is 88% at the nucleotide level and is 97% at the amino acid level, and there is one amino acid insertion. Using the cDNA clones as probe, five overlapping genomic clones have been isolated from a human phagemid DNA library. The GnT-V gene has been mapped to chromosome 2q21 using fluorescence in situ hybridization."}

{"project":"GGDB-2020","denotations":[{"id":"T1","span":{"begin":123,"end":128},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"T2","span":{"begin":315,"end":320},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"T3","span":{"begin":427,"end":432},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"T4","span":{"begin":513,"end":518},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"T5","span":{"begin":747,"end":752},"obj":"https://acgg.asia/db/ggdb/info/gg130"}],"text":"cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.\nHuman N-acetylglucosaminyltransferase V (GnT-V, EC 2.4.1.155) cDNA was isolated from a human fetal liver cDNA library. Oligonucleotide primers for polymerase chain reaction were designed according to the amino acid sequence of human GnT-V. Screening for the cDNA was carried out by plaque hybridization using PCR products of about 500 bp. Human GnT-V has 741 amino acids and six putative N-glycosylation sites. The homology to rat GnT-V is 88% at the nucleotide level and is 97% at the amino acid level, and there is one amino acid insertion. Using the cDNA clones as probe, five overlapping genomic clones have been isolated from a human phagemid DNA library. The GnT-V gene has been mapped to chromosome 2q21 using fluorescence in situ hybridization."}

{"project":"glycogenes","denotations":[{"id":"PD-GlycoGenes20190927-B_T1","span":{"begin":123,"end":128},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"PD-GlycoGenes20190927-B_T2","span":{"begin":260,"end":268},"obj":"url"},{"id":"PD-GlycoGenes20190927-B_T3","span":{"begin":315,"end":320},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"PD-GlycoGenes20190927-B_T4","span":{"begin":427,"end":432},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"PD-GlycoGenes20190927-B_T5","span":{"begin":433,"end":436},"obj":"https://acgg.asia/db/ggdb/info/gg135"},{"id":"PD-GlycoGenes20190927-B_T6","span":{"begin":513,"end":518},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"PD-GlycoGenes20190927-B_T7","span":{"begin":747,"end":752},"obj":"https://acgg.asia/db/ggdb/info/gg130"},{"id":"PD-GlycoGenes20190927-B_T8","span":{"begin":758,"end":761},"obj":"https://acgg.asia/db/ggdb/info/gg135"}],"text":"cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.\nHuman N-acetylglucosaminyltransferase V (GnT-V, EC 2.4.1.155) cDNA was isolated from a human fetal liver cDNA library. Oligonucleotide primers for polymerase chain reaction were designed according to the amino acid sequence of human GnT-V. Screening for the cDNA was carried out by plaque hybridization using PCR products of about 500 bp. Human GnT-V has 741 amino acids and six putative N-glycosylation sites. The homology to rat GnT-V is 88% at the nucleotide level and is 97% at the amino acid level, and there is one amino acid insertion. Using the cDNA clones as probe, five overlapping genomic clones have been isolated from a human phagemid DNA library. The GnT-V gene has been mapped to chromosome 2q21 using fluorescence in situ hybridization."}