| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-120 |
Sentence |
denotes |
Determination of tissue specificity of the enhancer by combinatorial operation of tissue-enriched transcription factors. |
| T2 |
121-231 |
Sentence |
denotes |
Both HNF-4 and C/EBP beta are required for liver-specific activity of the ornithine transcarbamylase enhancer. |
| T3 |
232-403 |
Sentence |
denotes |
The enhancer of the rat ornithine transcarbamylase gene is located 11 kilobases upstream from the transcription start site and has been shown to be hepatoma cell-specific. |
| T4 |
404-654 |
Sentence |
denotes |
Using transgenic mice, we showed that this enhancer is capable of activating transcription in a liver-specific manner, inverting the tissue specificity of the homologous promoter that is by itself more active in the small intestine than in the liver. |
| T5 |
655-994 |
Sentence |
denotes |
Transient transfection analysis with cultured hepatoma cells indicated that the enhancer activity resides in the approximately 110-base pair region containing four protein-binding sites, two for hepatocyte nuclear factor-4 (HNF-4) and two for CCAAT/enhancer binding protein (C/EBP), both of which are liver-selective transcription factors. |
| T6 |
995-1209 |
Sentence |
denotes |
Concatemerization of a region containing one HNF-4 and one C/EBP site led to reconstitution of the hepatoma cell-specific enhancer, and intactness of these two sites was strictly required for the enhancer activity. |
| T7 |
1210-1398 |
Sentence |
denotes |
Furthermore, cotransfection experiments showed that both HNF-4 and C/EBP beta are necessary, and neither alone sufficient, for activation of the reconstituted enhancer in nonhepatic cells. |
| T8 |
1399-1694 |
Sentence |
denotes |
Requirement of combinatorial operation of at least two liver-enriched transcription factors for transcriptional activation successfully explains why these liver-selective but not strictly liver-specific factors can confer more restricted liver specificity on transcription of their target genes. |
| T1 |
0-120 |
Sentence |
denotes |
Determination of tissue specificity of the enhancer by combinatorial operation of tissue-enriched transcription factors. |
| T2 |
121-231 |
Sentence |
denotes |
Both HNF-4 and C/EBP beta are required for liver-specific activity of the ornithine transcarbamylase enhancer. |
| T3 |
232-403 |
Sentence |
denotes |
The enhancer of the rat ornithine transcarbamylase gene is located 11 kilobases upstream from the transcription start site and has been shown to be hepatoma cell-specific. |
| T4 |
404-654 |
Sentence |
denotes |
Using transgenic mice, we showed that this enhancer is capable of activating transcription in a liver-specific manner, inverting the tissue specificity of the homologous promoter that is by itself more active in the small intestine than in the liver. |
| T5 |
655-994 |
Sentence |
denotes |
Transient transfection analysis with cultured hepatoma cells indicated that the enhancer activity resides in the approximately 110-base pair region containing four protein-binding sites, two for hepatocyte nuclear factor-4 (HNF-4) and two for CCAAT/enhancer binding protein (C/EBP), both of which are liver-selective transcription factors. |
| T6 |
995-1209 |
Sentence |
denotes |
Concatemerization of a region containing one HNF-4 and one C/EBP site led to reconstitution of the hepatoma cell-specific enhancer, and intactness of these two sites was strictly required for the enhancer activity. |
| T7 |
1210-1398 |
Sentence |
denotes |
Furthermore, cotransfection experiments showed that both HNF-4 and C/EBP beta are necessary, and neither alone sufficient, for activation of the reconstituted enhancer in nonhepatic cells. |
| T8 |
1399-1694 |
Sentence |
denotes |
Requirement of combinatorial operation of at least two liver-enriched transcription factors for transcriptional activation successfully explains why these liver-selective but not strictly liver-specific factors can confer more restricted liver specificity on transcription of their target genes. |
