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PubMed:8288586 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-80 Sentence denotes Transport of an export-defective protein by a highly hydrophobic signal peptide.
T2 81-265 Sentence denotes We have examined the sequence constraints on the amino-terminal region of the mature portion of alkaline phosphatase that are important for its efficient transport in Escherichia coli.
T3 266-390 Sentence denotes Using a homopolymeric sequence of serines to replace 6 residues in this region, a transport-incompetent mutant was produced.
T4 391-519 Sentence denotes Reintroduction of residues from the native sequence which restore charge and beta-turn potential resulted in little improvement.
T5 520-785 Sentence denotes However, by replacing the hydrophobic core of the signal peptide with a homopolymeric series of leucines, not only was transport restored but precursor processing was more efficient than for the wild type and was insensitive to disruption of the protonmotive force.
T6 786-879 Sentence denotes Moreover, we have titrated the signal peptide with leucine to alanine substitutions (Doud, S.
T7 880-892 Sentence denotes K., Chou, M.
T8 893-912 Sentence denotes M., and Kendall, D.
T9 913-1055 Sentence denotes A. (1993) Biochemistry 32, 1251-1256) and determined the minimum level of hydrophobicity necessary to achieve transport of the mutant protein.
T10 1056-1391 Sentence denotes The results indicate that signal peptide hydrophobicity can completely override possible requirements for negatively charged residues and strong beta-turn forming potential in the mature protein and that the polyleucine-containing signal peptide may act as a generic signal sequence for the transport of non-native proteins in E. coli.
T1 0-80 Sentence denotes Transport of an export-defective protein by a highly hydrophobic signal peptide.
T2 81-265 Sentence denotes We have examined the sequence constraints on the amino-terminal region of the mature portion of alkaline phosphatase that are important for its efficient transport in Escherichia coli.
T3 266-390 Sentence denotes Using a homopolymeric sequence of serines to replace 6 residues in this region, a transport-incompetent mutant was produced.
T4 391-519 Sentence denotes Reintroduction of residues from the native sequence which restore charge and beta-turn potential resulted in little improvement.
T5 520-785 Sentence denotes However, by replacing the hydrophobic core of the signal peptide with a homopolymeric series of leucines, not only was transport restored but precursor processing was more efficient than for the wild type and was insensitive to disruption of the protonmotive force.
T6 786-879 Sentence denotes Moreover, we have titrated the signal peptide with leucine to alanine substitutions (Doud, S.
T7 880-892 Sentence denotes K., Chou, M.
T8 893-912 Sentence denotes M., and Kendall, D.
T9 913-1055 Sentence denotes A. (1993) Biochemistry 32, 1251-1256) and determined the minimum level of hydrophobicity necessary to achieve transport of the mutant protein.
T10 1056-1391 Sentence denotes The results indicate that signal peptide hydrophobicity can completely override possible requirements for negatively charged residues and strong beta-turn forming potential in the mature protein and that the polyleucine-containing signal peptide may act as a generic signal sequence for the transport of non-native proteins in E. coli.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 248-264 OrganismTaxon denotes Escherichia coli 562
T2 1383-1390 OrganismTaxon denotes E. coli 562