Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-81 |
Sentence |
denotes |
Inhibition of thrombin receptor signaling by a G-protein coupled receptor kinase. |
T2 |
82-146 |
Sentence |
denotes |
Functional specificity among G-protein coupled receptor kinases. |
T3 |
147-396 |
Sentence |
denotes |
The thrombin receptor, a member of the seven membrane-spanning superfamily of G-protein coupled receptors, is activated by an irreversible proteolytic mechanism, but signaling by activated thrombin receptors shuts off soon after receptor activation. |
T4 |
397-564 |
Sentence |
denotes |
This shut-off mechanism is thought to be required for concentration-dependent responses to thrombin and an important determinant of the cell's sensitivity to thrombin. |
T5 |
565-711 |
Sentence |
denotes |
We report that the thrombin receptor is rapidly phosphorylated upon activation, consistent with the action of a G-protein-coupled receptor kinase. |
T6 |
712-873 |
Sentence |
denotes |
Moreover, the G-protein coupled receptor kinase BARK2 (beta-adrenergic receptor kinase 2) blocked signaling by thrombin receptors coexpressed in Xenopus oocytes. |
T7 |
874-969 |
Sentence |
denotes |
In this system, rhodopsin kinase was inactive and BARK1 was markedly less effective than BARK2. |
T8 |
970-1208 |
Sentence |
denotes |
Thrombin receptor mutants which lacked potential serine and threonine phosphorylation sites in the receptor's cytoplasmic tail were insensitive to inhibition by exogenous BARK2 but did confer concentration-dependent responses to thrombin. |
T9 |
1209-1380 |
Sentence |
denotes |
Our studies demonstrate that a G-protein coupled receptor kinase can shut off thrombin receptor signaling but that additional mechanism(s) for terminating signaling exist. |
T10 |
1381-1617 |
Sentence |
denotes |
These studies also reveal functional specificity among G-protein coupled receptor kinases in a novel in vivo reconstitution system and show that heterologous expression of these kinases can be used to manipulate cellular responsiveness. |
T1 |
0-81 |
Sentence |
denotes |
Inhibition of thrombin receptor signaling by a G-protein coupled receptor kinase. |
T2 |
82-146 |
Sentence |
denotes |
Functional specificity among G-protein coupled receptor kinases. |
T3 |
147-396 |
Sentence |
denotes |
The thrombin receptor, a member of the seven membrane-spanning superfamily of G-protein coupled receptors, is activated by an irreversible proteolytic mechanism, but signaling by activated thrombin receptors shuts off soon after receptor activation. |
T4 |
397-564 |
Sentence |
denotes |
This shut-off mechanism is thought to be required for concentration-dependent responses to thrombin and an important determinant of the cell's sensitivity to thrombin. |
T5 |
565-711 |
Sentence |
denotes |
We report that the thrombin receptor is rapidly phosphorylated upon activation, consistent with the action of a G-protein-coupled receptor kinase. |
T6 |
712-873 |
Sentence |
denotes |
Moreover, the G-protein coupled receptor kinase BARK2 (beta-adrenergic receptor kinase 2) blocked signaling by thrombin receptors coexpressed in Xenopus oocytes. |
T7 |
874-969 |
Sentence |
denotes |
In this system, rhodopsin kinase was inactive and BARK1 was markedly less effective than BARK2. |
T8 |
970-1208 |
Sentence |
denotes |
Thrombin receptor mutants which lacked potential serine and threonine phosphorylation sites in the receptor's cytoplasmic tail were insensitive to inhibition by exogenous BARK2 but did confer concentration-dependent responses to thrombin. |
T9 |
1209-1380 |
Sentence |
denotes |
Our studies demonstrate that a G-protein coupled receptor kinase can shut off thrombin receptor signaling but that additional mechanism(s) for terminating signaling exist. |
T10 |
1381-1617 |
Sentence |
denotes |
These studies also reveal functional specificity among G-protein coupled receptor kinases in a novel in vivo reconstitution system and show that heterologous expression of these kinases can be used to manipulate cellular responsiveness. |