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PubMed:8288567 JSONTXT

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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 1187-1194 Glycan_Motif denotes Heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO

sentences

Id Subject Object Predicate Lexical cue
T1 0-93 Sentence denotes Expression, purification, and characterization of the G protein-coupled receptor kinase GRK5.
T2 94-322 Sentence denotes G protein-coupled receptor kinases (GRKs) such as rhodopsin kinase and the beta-adrenergic receptor kinase (beta ARK) play an important role in agonist-specific phosphorylation and desensitization of G protein-coupled receptors.
T3 323-445 Sentence denotes GRK5 is a recently identified member of the GRK family that has greater homology with rhodopsin kinase than with beta ARK.
T4 446-614 Sentence denotes To further characterize the activity of GRK5, it has been overexpressed in Sf9 insect cells and purified by successive chromatography on S-Sepharose and Mono S columns.
T5 615-1003 Sentence denotes GRK5 phosphorylates the beta 2-adrenergic receptor (beta 2AR), m2 muscarinic cholinergic receptor, and rhodopsin in an agonist-dependent manner to maximal stoichiometries of approximately 2.5, 1.5, and 1 mol of phosphate/mol of receptor, respectively, with Km values of approximately 0.5 microM for the beta 2AR, approximately 16 microM for rhodopsin, and approximately 24 microM for ATP.
T6 1004-1186 Sentence denotes Peptide phosphorylation studies suggest that in contrast to beta ARK and rhodopsin kinase, GRK5 preferentially phosphorylates on nonacidic peptides with a Km of approximately 1.5 mM.
T7 1187-1367 Sentence denotes Heparin and dextran sulfate were found to be potent inhibitors of GRK5 with IC50 values of approximately 1 nM, thereby being at least 150-fold more potent on GRK5 than on beta ARK.
T8 1368-1484 Sentence denotes GRK5 can also be activated by polycations, with 10 microM polylysine promoting an approximately 2.6-fold activation.
T9 1485-1686 Sentence denotes Overall, these studies demonstrate that GRK5 has unique properties that distinguish it from other members of the GRK family and that likely play an important role in modulating its mechanism of action.
T1 0-93 Sentence denotes Expression, purification, and characterization of the G protein-coupled receptor kinase GRK5.
T2 94-322 Sentence denotes G protein-coupled receptor kinases (GRKs) such as rhodopsin kinase and the beta-adrenergic receptor kinase (beta ARK) play an important role in agonist-specific phosphorylation and desensitization of G protein-coupled receptors.
T3 323-445 Sentence denotes GRK5 is a recently identified member of the GRK family that has greater homology with rhodopsin kinase than with beta ARK.
T4 446-614 Sentence denotes To further characterize the activity of GRK5, it has been overexpressed in Sf9 insect cells and purified by successive chromatography on S-Sepharose and Mono S columns.
T5 615-1003 Sentence denotes GRK5 phosphorylates the beta 2-adrenergic receptor (beta 2AR), m2 muscarinic cholinergic receptor, and rhodopsin in an agonist-dependent manner to maximal stoichiometries of approximately 2.5, 1.5, and 1 mol of phosphate/mol of receptor, respectively, with Km values of approximately 0.5 microM for the beta 2AR, approximately 16 microM for rhodopsin, and approximately 24 microM for ATP.
T6 1004-1186 Sentence denotes Peptide phosphorylation studies suggest that in contrast to beta ARK and rhodopsin kinase, GRK5 preferentially phosphorylates on nonacidic peptides with a Km of approximately 1.5 mM.
T7 1187-1367 Sentence denotes Heparin and dextran sulfate were found to be potent inhibitors of GRK5 with IC50 values of approximately 1 nM, thereby being at least 150-fold more potent on GRK5 than on beta ARK.
T8 1368-1484 Sentence denotes GRK5 can also be activated by polycations, with 10 microM polylysine promoting an approximately 2.6-fold activation.
T9 1485-1686 Sentence denotes Overall, these studies demonstrate that GRK5 has unique properties that distinguish it from other members of the GRK family and that likely play an important role in modulating its mechanism of action.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 1187-1194 https://glytoucan.org/Structures/Glycans/G00021MO denotes Heparin
T2 1187-1194 https://glytoucan.org/Structures/Glycans/G54161DR denotes Heparin