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sentences

Id Subject Object Predicate Lexical cue
T1 0-138 Sentence denotes 1H NMR study of the solution molecular and electronic structure of engineered distal myoglobin His64(E7) Val/Val68(E11) His double mutant.
T2 139-226 Sentence denotes Coordination of His64(E11) at the sixth position in both low-spin and high-spin states.
T3 227-550 Sentence denotes A genetically engineered human myoglobin (Mb) in which the distal His, His64(E7), and the distal Val, Val68(E11), are replaced by Val and His, respectively, has been expressed in Escherichia coli, for the purpose of assessing the potential role of a E11 residue in providing a hydrogen bond donor to the coordinated ligand.
T4 551-617 Sentence denotes Molecular modeling indicates that such an interaction is possible.
T5 618-918 Sentence denotes The 1H NMR spectrum of the ferric form of the double mutant Mb exhibits large hyperfine shifts and strong paramagnetic relaxation for which the temperature dependence of the hyperfine shifts reveals a thermal equilibrium between a low-spin and high-spin state (70, 30% at 25 degrees C, respectively).
T6 919-1125 Sentence denotes Standard sequence specific two-dimensional (2D) NMR assignments of the E and F helical backbones allow the identification of the peptide protons for the proximal His93(F8) and substituted distal His68(E11).
T7 1126-1342 Sentence denotes Steady-state nuclear Overhauser effect from these peptide protons locate strongly hyperfine shifted His93(F8) and His68(E11) side chain protons which dictate that both the imidazole rings are coordinated to the iron.
T8 1343-1468 Sentence denotes 2D bond correlation and one-dimensional and 2D dipolar correlation experiments locate and assign the resonances for the heme.
T9 1469-1731 Sentence denotes The pattern of the heme contact shifts in both the low-spin and high-spin state, together with the nature of the temperature dependence of the His93(F8) and His68(E11) resonances, establish that the two His are ligated in the high-spin as well as low-spin forms.
T10 1732-2043 Sentence denotes The pattern of heme methyl hyperfine shifts in the low-spin state, and the smaller hyperfine shifts for His68(E11) as compared to His93(F8) in the high-spin state, indicate that the axial bond to the distal His68(E11) is weakened or strained as compared with that for the proximal His93(F8) in both spin states.
T11 2044-2247 Sentence denotes This weak ligation originates from a tilted iron-His68 bond, the only conformation in which His68 can place its imidazole group sufficiently close to bind to the heme iron in the conventional Mb folding.
T12 2248-2566 Sentence denotes Not only do these results support the belief that distal His is indispensable for the control of the ligand binding in Mb and hemoglobin, but also reveal the significance of the evolution that the stereochemical disposition of both His64 and Val68 are unique and non-exchangeable for interacting with the bound ligand.
T1 0-138 Sentence denotes 1H NMR study of the solution molecular and electronic structure of engineered distal myoglobin His64(E7) Val/Val68(E11) His double mutant.
T2 139-226 Sentence denotes Coordination of His64(E11) at the sixth position in both low-spin and high-spin states.
T3 227-550 Sentence denotes A genetically engineered human myoglobin (Mb) in which the distal His, His64(E7), and the distal Val, Val68(E11), are replaced by Val and His, respectively, has been expressed in Escherichia coli, for the purpose of assessing the potential role of a E11 residue in providing a hydrogen bond donor to the coordinated ligand.
T4 551-617 Sentence denotes Molecular modeling indicates that such an interaction is possible.
T5 618-918 Sentence denotes The 1H NMR spectrum of the ferric form of the double mutant Mb exhibits large hyperfine shifts and strong paramagnetic relaxation for which the temperature dependence of the hyperfine shifts reveals a thermal equilibrium between a low-spin and high-spin state (70, 30% at 25 degrees C, respectively).
T6 919-1125 Sentence denotes Standard sequence specific two-dimensional (2D) NMR assignments of the E and F helical backbones allow the identification of the peptide protons for the proximal His93(F8) and substituted distal His68(E11).
T7 1126-1342 Sentence denotes Steady-state nuclear Overhauser effect from these peptide protons locate strongly hyperfine shifted His93(F8) and His68(E11) side chain protons which dictate that both the imidazole rings are coordinated to the iron.
T8 1343-1468 Sentence denotes 2D bond correlation and one-dimensional and 2D dipolar correlation experiments locate and assign the resonances for the heme.
T9 1469-1731 Sentence denotes The pattern of the heme contact shifts in both the low-spin and high-spin state, together with the nature of the temperature dependence of the His93(F8) and His68(E11) resonances, establish that the two His are ligated in the high-spin as well as low-spin forms.
T10 1732-2043 Sentence denotes The pattern of heme methyl hyperfine shifts in the low-spin state, and the smaller hyperfine shifts for His68(E11) as compared to His93(F8) in the high-spin state, indicate that the axial bond to the distal His68(E11) is weakened or strained as compared with that for the proximal His93(F8) in both spin states.
T11 2044-2247 Sentence denotes This weak ligation originates from a tilted iron-His68 bond, the only conformation in which His68 can place its imidazole group sufficiently close to bind to the heme iron in the conventional Mb folding.
T12 2248-2566 Sentence denotes Not only do these results support the belief that distal His is indispensable for the control of the ligand binding in Mb and hemoglobin, but also reveal the significance of the evolution that the stereochemical disposition of both His64 and Val68 are unique and non-exchangeable for interacting with the bound ligand.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 1072-1080 http://purl.obolibrary.org/obo/MAT_0000491 denotes proximal
T2 2004-2012 http://purl.obolibrary.org/obo/MAT_0000491 denotes proximal

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 1072-1080 Body_part denotes proximal http://purl.obolibrary.org/obo/MAT_0000491
T2 2004-2012 Body_part denotes proximal http://purl.obolibrary.org/obo/MAT_0000491

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 252-257 OrganismTaxon denotes human 9606
T2 406-422 OrganismTaxon denotes Escherichia coli 562

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1006-1015 Body_part denotes backbones http://purl.obolibrary.org/obo/UBERON_0001130