> top > docs > PubMed:8288557 > annotations

PubMed:8288557 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes Kinetic studies on the removal of extracellular hydrogen peroxide by cultured fibroblasts.
T2 91-238 Sentence denotes To investigate the function of antioxidant enzymes in intact cells, we examined the removal of extracellular H2O2 by cultured fibroblasts (IMR-90).
T3 239-406 Sentence denotes H2O2 concentration dependence of the reaction rate was interpreted as that the process involves two kinetically different reactions (referred to as reactions 1 and 2).
T4 407-554 Sentence denotes Reaction 1 was characterized by a relatively low Km value (about 40 microM), and reaction 2 by linear dependence of the rate up to 500 microM H2O2.
T5 555-734 Sentence denotes The magnitude of reaction 1 was reduced by treatment of the cells with diethyl maleate or 6-amino-nicotinamide, while reaction 2 was inhibited by 3-amino-1,2,4-triazole treatment.
T6 735-840 Sentence denotes It was concluded that reactions 1 and 2 are principally due to GSH peroxidase and catalase, respectively.
T7 841-1026 Sentence denotes The values of kinetic parameters were estimated by curve-fitting, and it was inferred that 80 to 90% of H2O2 is decomposed by GSH peroxidase at H2O2 concentrations lower than 10 microM.
T8 1027-1106 Sentence denotes The contribution of catalase increases with the increase in H2O2 concentration.
T9 1107-1229 Sentence denotes The intact cells showed a low catalase activity (about 15%), as compared with the activity found in the solubilized cells.
T10 1230-1336 Sentence denotes The low catalase activity was ascribed to the latency of the enzyme caused by localization in peroxisomes.
T11 1337-1404 Sentence denotes Fibroblasts also removed intracellular H2O2 generated by menadione.
T12 1405-1521 Sentence denotes Treatment with diethyl maleate greatly impaired the H2O2-removing capability and caused H2O2 efflux into the medium.
T1 0-90 Sentence denotes Kinetic studies on the removal of extracellular hydrogen peroxide by cultured fibroblasts.
T2 91-238 Sentence denotes To investigate the function of antioxidant enzymes in intact cells, we examined the removal of extracellular H2O2 by cultured fibroblasts (IMR-90).
T3 239-406 Sentence denotes H2O2 concentration dependence of the reaction rate was interpreted as that the process involves two kinetically different reactions (referred to as reactions 1 and 2).
T4 407-554 Sentence denotes Reaction 1 was characterized by a relatively low Km value (about 40 microM), and reaction 2 by linear dependence of the rate up to 500 microM H2O2.
T5 555-734 Sentence denotes The magnitude of reaction 1 was reduced by treatment of the cells with diethyl maleate or 6-amino-nicotinamide, while reaction 2 was inhibited by 3-amino-1,2,4-triazole treatment.
T6 735-840 Sentence denotes It was concluded that reactions 1 and 2 are principally due to GSH peroxidase and catalase, respectively.
T7 841-1026 Sentence denotes The values of kinetic parameters were estimated by curve-fitting, and it was inferred that 80 to 90% of H2O2 is decomposed by GSH peroxidase at H2O2 concentrations lower than 10 microM.
T8 1027-1106 Sentence denotes The contribution of catalase increases with the increase in H2O2 concentration.
T9 1107-1229 Sentence denotes The intact cells showed a low catalase activity (about 15%), as compared with the activity found in the solubilized cells.
T10 1230-1336 Sentence denotes The low catalase activity was ascribed to the latency of the enzyme caused by localization in peroxisomes.
T11 1337-1404 Sentence denotes Fibroblasts also removed intracellular H2O2 generated by menadione.
T12 1405-1521 Sentence denotes Treatment with diethyl maleate greatly impaired the H2O2-removing capability and caused H2O2 efflux into the medium.

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 34-47 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576
T2 78-89 Body_part denotes fibroblasts http://purl.obolibrary.org/obo/CL_0000057
T3 186-199 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576
T4 217-228 Body_part denotes fibroblasts http://purl.obolibrary.org/obo/CL_0000057
T5 1337-1348 Body_part denotes Fibroblasts http://purl.obolibrary.org/obo/CL_0000057
T6 1362-1375 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 78-89 Cell denotes fibroblasts http://purl.obolibrary.org/obo/CL:0000057
T2 217-228 Cell denotes fibroblasts http://purl.obolibrary.org/obo/CL:0000057
T3 1337-1348 Cell denotes Fibroblasts http://purl.obolibrary.org/obo/CL:0000057