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PubMed:8276878 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-104 Sentence denotes The REC1 gene of Ustilago maydis involved in the cellular response to DNA damage encodes an exonuclease.
T2 105-277 Sentence denotes Mutation in the REC1 gene of Ustilago maydis is known to lead to a complex phenotype with alterations in DNA repair, recombination, mutagenesis, meiosis, and cell division.
T3 278-463 Sentence denotes The predicted product of the REC1 gene is a polypeptide of 522 amino acid residues with a molecular mass of 56,866 daltons, with no overall sequence homology to any other known protein.
T4 464-611 Sentence denotes The open reading frame of the REC1 gene placed by itself in a U. maydis expression vector was found to be sufficient to complement the rec1 mutant.
T5 612-744 Sentence denotes Overexpression of REC1 in Escherichia coli gave rise to the anticipated 57-kDa product together with a 3'-->5' exonuclease activity.
T6 745-1026 Sentence denotes This activity was only present in cells overexpressing REC1 and its characteristics were distinguishable from the major bacterial nucleases, but it had certain enzymatic features in common with epsilon, the proofreading exonuclease subunit of E. coli DNA polymerase III holoenzyme.
T7 1027-1294 Sentence denotes To facilitate isolation of the protein product from bacteria, the REC1 gene was overexpressed from a vector that fused a hexa-histidine-leader sequence onto the amino terminus, enabling the isolation of the HisREC1 product on an immobilized metal ion affinity column.
T8 1295-1362 Sentence denotes The His-REC1 protein co-eluted with the novel exonuclease activity.
T9 1363-1526 Sentence denotes Alignment of the amino acid sequence of the REC1 gene product with the conserved proofreading exonuclease motifs of DNA polymerases indicated significant homology.
T1 0-104 Sentence denotes The REC1 gene of Ustilago maydis involved in the cellular response to DNA damage encodes an exonuclease.
T2 105-277 Sentence denotes Mutation in the REC1 gene of Ustilago maydis is known to lead to a complex phenotype with alterations in DNA repair, recombination, mutagenesis, meiosis, and cell division.
T3 278-463 Sentence denotes The predicted product of the REC1 gene is a polypeptide of 522 amino acid residues with a molecular mass of 56,866 daltons, with no overall sequence homology to any other known protein.
T4 464-611 Sentence denotes The open reading frame of the REC1 gene placed by itself in a U. maydis expression vector was found to be sufficient to complement the rec1 mutant.
T5 612-744 Sentence denotes Overexpression of REC1 in Escherichia coli gave rise to the anticipated 57-kDa product together with a 3'-->5' exonuclease activity.
T6 745-1026 Sentence denotes This activity was only present in cells overexpressing REC1 and its characteristics were distinguishable from the major bacterial nucleases, but it had certain enzymatic features in common with epsilon, the proofreading exonuclease subunit of E. coli DNA polymerase III holoenzyme.
T7 1027-1294 Sentence denotes To facilitate isolation of the protein product from bacteria, the REC1 gene was overexpressed from a vector that fused a hexa-histidine-leader sequence onto the amino terminus, enabling the isolation of the HisREC1 product on an immobilized metal ion affinity column.
T8 1295-1362 Sentence denotes The His-REC1 protein co-eluted with the novel exonuclease activity.
T9 1363-1526 Sentence denotes Alignment of the amino acid sequence of the REC1 gene product with the conserved proofreading exonuclease motifs of DNA polymerases indicated significant homology.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 17-32 OrganismTaxon denotes Ustilago maydis 5270
T2 134-149 OrganismTaxon denotes Ustilago maydis 5270
T3 638-654 OrganismTaxon denotes Escherichia coli 562
T4 988-995 OrganismTaxon denotes E. coli 562
T5 1079-1087 OrganismTaxon denotes bacteria 2|629395

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 263-276 Body_part denotes cell division http://purl.obolibrary.org/obo/UBERON_2000098

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 939-946 Cell denotes epsilon http://purl.obolibrary.org/obo/CL:0003025