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sentences

Id Subject Object Predicate Lexical cue
T1 0-46 Sentence denotes Structure of ATP citrate lyase from rat liver.
T2 47-100 Sentence denotes Physicochemical studies and proteolytic modification.
T3 101-268 Sentence denotes ATP citrate lyase was purified by two different procedures from the livers of rats first starved and then fed with a fat-deficient and high carbohydrate-glycerol diet.
T4 269-387 Sentence denotes These enzyme preparations were judged homogeneous by sedimentation equilibrium and polyacrylamide gel electrophoresis.
T5 388-496 Sentence denotes The molecular weight of the native enzyme was around 4.4 X 10(5) as determined by sedimentation equilibrium.
T6 497-641 Sentence denotes On sodium dodecyl sulfate gel electrophoresis the enzyme usually showed a single protein band with an estimated molecular weight of 1.2 X 10(5).
T7 642-787 Sentence denotes A similar value for the molecular weight of the subunit was obtained by gel filtration on 6% agarose in the presence of 6 M guanidinium chloride.
T8 788-903 Sentence denotes The molecular weight of this polypeptide chain was estimated by sedimentation equilibrium to be around 1.1 X 10(5).
T9 904-1012 Sentence denotes These results indicated that ATP citrate lyase has a subunit structure of four polypeptides of similar size.
T10 1013-1108 Sentence denotes The extinction coefficient of the dry protein and its amino acid composition are also reported.
T11 1109-1374 Sentence denotes Some batches of fully active enzyme, judged to be homogeneous by sedimentation equilibrium and polyacrylamide gel electrophoresis, showed two additional major polypeptides (Mr approximately 7.1 X 10(4) and 5.5 X 10(4)) on sodium dodecyl sulfate gel electrophoresis.
T12 1375-1700 Sentence denotes Studies on the polypeptides produced by proteolytic modification of the native enzyme by trypsin indicated that the additional protein bands observed on sodium dodecyl sulfate gel electrophoresis with some of the batches of enzyme could have been formed by limited proteolysis ("nicking") of the original 1.1 X 10(5) subunit.
T13 1701-1842 Sentence denotes Trypsin treatment of the native enzyme did not affect the enzyme activity, whereas chymotrypsin and pronase treatment inactivated the enzyme.
T14 1843-2133 Sentence denotes The trypsin-treated enzyme, which contained only the two smaller polypeptides, did not differ significantly from the untreated enzyme with respect to sedimentation behavior, phosphorylation by ATP, Km for citrate, and immunoreactivity, but it was more heat-labile than the untreated enzyme.
T15 2134-2239 Sentence denotes The phosphate group on the phosphorylated "nicked" enzyme was located on the larger polypeptide fragment.
T1 0-46 Sentence denotes Structure of ATP citrate lyase from rat liver.
T2 47-100 Sentence denotes Physicochemical studies and proteolytic modification.
T3 101-268 Sentence denotes ATP citrate lyase was purified by two different procedures from the livers of rats first starved and then fed with a fat-deficient and high carbohydrate-glycerol diet.
T4 269-387 Sentence denotes These enzyme preparations were judged homogeneous by sedimentation equilibrium and polyacrylamide gel electrophoresis.
T5 388-496 Sentence denotes The molecular weight of the native enzyme was around 4.4 X 10(5) as determined by sedimentation equilibrium.
T6 497-641 Sentence denotes On sodium dodecyl sulfate gel electrophoresis the enzyme usually showed a single protein band with an estimated molecular weight of 1.2 X 10(5).
T7 642-787 Sentence denotes A similar value for the molecular weight of the subunit was obtained by gel filtration on 6% agarose in the presence of 6 M guanidinium chloride.
T8 788-903 Sentence denotes The molecular weight of this polypeptide chain was estimated by sedimentation equilibrium to be around 1.1 X 10(5).
T9 904-1012 Sentence denotes These results indicated that ATP citrate lyase has a subunit structure of four polypeptides of similar size.
T10 1013-1108 Sentence denotes The extinction coefficient of the dry protein and its amino acid composition are also reported.
T11 1109-1374 Sentence denotes Some batches of fully active enzyme, judged to be homogeneous by sedimentation equilibrium and polyacrylamide gel electrophoresis, showed two additional major polypeptides (Mr approximately 7.1 X 10(4) and 5.5 X 10(4)) on sodium dodecyl sulfate gel electrophoresis.
T12 1375-1700 Sentence denotes Studies on the polypeptides produced by proteolytic modification of the native enzyme by trypsin indicated that the additional protein bands observed on sodium dodecyl sulfate gel electrophoresis with some of the batches of enzyme could have been formed by limited proteolysis ("nicking") of the original 1.1 X 10(5) subunit.
T13 1701-1842 Sentence denotes Trypsin treatment of the native enzyme did not affect the enzyme activity, whereas chymotrypsin and pronase treatment inactivated the enzyme.
T14 1843-2133 Sentence denotes The trypsin-treated enzyme, which contained only the two smaller polypeptides, did not differ significantly from the untreated enzyme with respect to sedimentation behavior, phosphorylation by ATP, Km for citrate, and immunoreactivity, but it was more heat-labile than the untreated enzyme.
T15 2134-2239 Sentence denotes The phosphate group on the phosphorylated "nicked" enzyme was located on the larger polypeptide fragment.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 40-45 http://purl.obolibrary.org/obo/MAT_0000097 denotes liver
T2 169-175 http://purl.obolibrary.org/obo/MAT_0000097 denotes livers

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 40-45 Body_part denotes liver http://purl.obolibrary.org/obo/MAT_0000097
T2 169-175 Body_part denotes livers http://purl.obolibrary.org/obo/MAT_0000097

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 36-39 OrganismTaxon denotes rat 10114|10116
T3 179-183 OrganismTaxon denotes rats 10114|10116

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 40-45 Body_part denotes liver http://purl.obolibrary.org/obo/UBERON_0002107
T2 218-221 Body_part denotes fat http://purl.obolibrary.org/obo/UBERON_0001013

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 586-590 Cell denotes band http://purl.obolibrary.org/obo/CL:0000560