PubMed:821946 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":117},"obj":"Sentence"},{"id":"T2","span":{"begin":118,"end":213},"obj":"Sentence"},{"id":"T3","span":{"begin":214,"end":435},"obj":"Sentence"},{"id":"T4","span":{"begin":436,"end":648},"obj":"Sentence"},{"id":"T5","span":{"begin":649,"end":743},"obj":"Sentence"},{"id":"T6","span":{"begin":744,"end":883},"obj":"Sentence"},{"id":"T7","span":{"begin":884,"end":968},"obj":"Sentence"},{"id":"T8","span":{"begin":969,"end":1140},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":117},"obj":"Sentence"},{"id":"T2","span":{"begin":118,"end":213},"obj":"Sentence"},{"id":"T3","span":{"begin":214,"end":435},"obj":"Sentence"},{"id":"T4","span":{"begin":436,"end":648},"obj":"Sentence"},{"id":"T5","span":{"begin":649,"end":743},"obj":"Sentence"},{"id":"T6","span":{"begin":744,"end":883},"obj":"Sentence"},{"id":"T7","span":{"begin":884,"end":968},"obj":"Sentence"},{"id":"T8","span":{"begin":969,"end":1140},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica.\nMethods have been developed for identifying the pathway of assimilation of N2-derived nitrogen. The products of fixation of 13N-labeled nitrogen gas ([13N]N2), and the distribution of 13N within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. Ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of glutamate, in that order, were the first observed products of fixation of N2 by the cyanobacterium (blue-green alga), Anabaena cylindrica. This sequence of the formation of nitrogenous products was confirmed by the use of inhibitors. The presence of 1 mM methionine sulfoximine permitted continued formation of 13NH3, while virtually preventing 13N-labeling of amino acids. In the presence of 1 mM azaserine, glutamine was labeled, but not other amino acids. Our observations demonstrate unequivocally that N2-derived nitrogen fixed by this organism is metabolized initially by the glutamine synthetase/glutamate synthase pathway."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":81,"end":95},"obj":"http://purl.obolibrary.org/obo/MAT_0000192"},{"id":"T2","span":{"begin":594,"end":608},"obj":"http://purl.obolibrary.org/obo/MAT_0000192"}],"text":"Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica.\nMethods have been developed for identifying the pathway of assimilation of N2-derived nitrogen. The products of fixation of 13N-labeled nitrogen gas ([13N]N2), and the distribution of 13N within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. Ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of glutamate, in that order, were the first observed products of fixation of N2 by the cyanobacterium (blue-green alga), Anabaena cylindrica. This sequence of the formation of nitrogenous products was confirmed by the use of inhibitors. The presence of 1 mM methionine sulfoximine permitted continued formation of 13NH3, while virtually preventing 13N-labeling of amino acids. In the presence of 1 mM azaserine, glutamine was labeled, but not other amino acids. Our observations demonstrate unequivocally that N2-derived nitrogen fixed by this organism is metabolized initially by the glutamine synthetase/glutamate synthase pathway."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":81,"end":95},"obj":"Body_part"},{"id":"T2","span":{"begin":594,"end":608},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000192"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000192"}],"text":"Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica.\nMethods have been developed for identifying the pathway of assimilation of N2-derived nitrogen. The products of fixation of 13N-labeled nitrogen gas ([13N]N2), and the distribution of 13N within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. Ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of glutamate, in that order, were the first observed products of fixation of N2 by the cyanobacterium (blue-green alga), Anabaena cylindrica. This sequence of the formation of nitrogenous products was confirmed by the use of inhibitors. The presence of 1 mM methionine sulfoximine permitted continued formation of 13NH3, while virtually preventing 13N-labeling of amino acids. In the presence of 1 mM azaserine, glutamine was labeled, but not other amino acids. Our observations demonstrate unequivocally that N2-derived nitrogen fixed by this organism is metabolized initially by the glutamine synthetase/glutamate synthase pathway."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":81,"end":95},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":97,"end":116},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":436,"end":443},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":594,"end":608},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":628,"end":647},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"102234"},{"id":"A2","pred":"db_id","subj":"T2","obj":"1165"},{"id":"A3","pred":"db_id","subj":"T3","obj":"29189"},{"id":"A4","pred":"db_id","subj":"T4","obj":"102234"},{"id":"A5","pred":"db_id","subj":"T5","obj":"1165"}],"text":"Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica.\nMethods have been developed for identifying the pathway of assimilation of N2-derived nitrogen. The products of fixation of 13N-labeled nitrogen gas ([13N]N2), and the distribution of 13N within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. Ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of glutamate, in that order, were the first observed products of fixation of N2 by the cyanobacterium (blue-green alga), Anabaena cylindrica. This sequence of the formation of nitrogenous products was confirmed by the use of inhibitors. The presence of 1 mM methionine sulfoximine permitted continued formation of 13NH3, while virtually preventing 13N-labeling of amino acids. In the presence of 1 mM azaserine, glutamine was labeled, but not other amino acids. Our observations demonstrate unequivocally that N2-derived nitrogen fixed by this organism is metabolized initially by the glutamine synthetase/glutamate synthase pathway."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1051,"end":1059},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000468"}],"text":"Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica.\nMethods have been developed for identifying the pathway of assimilation of N2-derived nitrogen. The products of fixation of 13N-labeled nitrogen gas ([13N]N2), and the distribution of 13N within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. Ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of glutamate, in that order, were the first observed products of fixation of N2 by the cyanobacterium (blue-green alga), Anabaena cylindrica. This sequence of the formation of nitrogenous products was confirmed by the use of inhibitors. The presence of 1 mM methionine sulfoximine permitted continued formation of 13NH3, while virtually preventing 13N-labeling of amino acids. In the presence of 1 mM azaserine, glutamine was labeled, but not other amino acids. Our observations demonstrate unequivocally that N2-derived nitrogen fixed by this organism is metabolized initially by the glutamine synthetase/glutamate synthase pathway."}