PubMed:8211112 JSONTXT

{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":41,"end":45},"obj":"protein"},{"id":"T2","span":{"begin":51,"end":59},"obj":"protein"},{"id":"T3","span":{"begin":103,"end":107},"obj":"protein"},{"id":"T4","span":{"begin":146,"end":180},"obj":"protein"},{"id":"T5","span":{"begin":277,"end":281},"obj":"protein"},{"id":"T6","span":{"begin":343,"end":351},"obj":"protein"},{"id":"T7","span":{"begin":419,"end":423},"obj":"protein"},{"id":"T8","span":{"begin":464,"end":472},"obj":"protein"},{"id":"T9","span":{"begin":496,"end":500},"obj":"protein"},{"id":"T10","span":{"begin":516,"end":524},"obj":"protein"},{"id":"T11","span":{"begin":544,"end":548},"obj":"protein"},{"id":"T12","span":{"begin":588,"end":596},"obj":"protein"},{"id":"T13","span":{"begin":628,"end":632},"obj":"protein"},{"id":"T14","span":{"begin":637,"end":641},"obj":"protein"},{"id":"T15","span":{"begin":666,"end":674},"obj":"protein"},{"id":"T16","span":{"begin":739,"end":743},"obj":"protein"},{"id":"T17","span":{"begin":785,"end":789},"obj":"protein"}],"text":"Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9.\nIn the assembly of a prespliceosome, U2 small nuclear ribonucleoprotein (snRNP) functions in pre-messenger RNA (mRNA) splicing together with splicing factors (SFs) 3a, SF3b, and several other proteins. The 17S but not the 12S form of U2 snRNP is active in splicing-complex formation. Here it is shown that the SF3a subunits correspond to three of the 17S U2 snRNP-specific polypeptides. SF3a interacts with U2 snRNP in the presence of SF3b to generate a structure similar to 17S U2 snRNP, which suggests a function for SF3a and SF3b in the incorporation of U2 snRNP into the spliceosome. Furthermore, the 60-kilodalton subunit of SF3a is related to the yeast splicing protein PRP9."}