| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-153 |
Sentence |
denotes |
Analysis of mutations and expression of GAP-related domain of the neurofibromatosis type 1 (NF1) gene in the progression of chronic myelogenous leukemia. |
| TextSentencer_T2 |
154-346 |
Sentence |
denotes |
We investigated mutations in the GTPase activating protein-related domain of the neurofibromatosis type 1 gene (NF1-GRD) and its expression in each phase of chronic myelogenous leukemia (CML). |
| TextSentencer_T3 |
347-585 |
Sentence |
denotes |
Samples from 45 cases in chronic phase (CP), 41 in acute phase, and four CML cell lines were examined for mutations in the NF1-GRD by single-strand conformation polymorphism (SSCP) analysis and allele specific restriction analysis (ASRA). |
| TextSentencer_T4 |
586-715 |
Sentence |
denotes |
No mutations were detected in the exon where frequent mutations have recently been reported in human tumors, namely the FLR exon. |
| TextSentencer_T5 |
716-801 |
Sentence |
denotes |
We also examined for point mutations of the N-ras gene but found no mutations either. |
| TextSentencer_T6 |
802-1110 |
Sentence |
denotes |
In 23 samples from CML cases and four CML cell lines, expression of two types of the NF1-GRD transcripts, type I and type II, were examined by NF1-GRD-specific polymerase chain reaction-based densitometric analysis and by the quantitative assay with coamplification of the NF1-GRD and beta-actin transcripts. |
| TextSentencer_T7 |
1111-1353 |
Sentence |
denotes |
Consequently, although expression level of type I transcripts varied among the samples, type II expression was increased in CML cell lines and a minor increase in type II expression was observed in the samples in acute phase compared with CP. |
| TextSentencer_T8 |
1354-1567 |
Sentence |
denotes |
However, this difference in type II expression between CP and acute phase was so small that changes of NF1-GRD transcripts as well as NF1-GRD or N-ras mutations might not be responsible for the progression of CML. |
| T1 |
0-153 |
Sentence |
denotes |
Analysis of mutations and expression of GAP-related domain of the neurofibromatosis type 1 (NF1) gene in the progression of chronic myelogenous leukemia. |
| T2 |
154-346 |
Sentence |
denotes |
We investigated mutations in the GTPase activating protein-related domain of the neurofibromatosis type 1 gene (NF1-GRD) and its expression in each phase of chronic myelogenous leukemia (CML). |
| T3 |
347-585 |
Sentence |
denotes |
Samples from 45 cases in chronic phase (CP), 41 in acute phase, and four CML cell lines were examined for mutations in the NF1-GRD by single-strand conformation polymorphism (SSCP) analysis and allele specific restriction analysis (ASRA). |
| T4 |
586-715 |
Sentence |
denotes |
No mutations were detected in the exon where frequent mutations have recently been reported in human tumors, namely the FLR exon. |
| T5 |
716-801 |
Sentence |
denotes |
We also examined for point mutations of the N-ras gene but found no mutations either. |
| T6 |
802-1110 |
Sentence |
denotes |
In 23 samples from CML cases and four CML cell lines, expression of two types of the NF1-GRD transcripts, type I and type II, were examined by NF1-GRD-specific polymerase chain reaction-based densitometric analysis and by the quantitative assay with coamplification of the NF1-GRD and beta-actin transcripts. |
| T7 |
1111-1353 |
Sentence |
denotes |
Consequently, although expression level of type I transcripts varied among the samples, type II expression was increased in CML cell lines and a minor increase in type II expression was observed in the samples in acute phase compared with CP. |
| T8 |
1354-1567 |
Sentence |
denotes |
However, this difference in type II expression between CP and acute phase was so small that changes of NF1-GRD transcripts as well as NF1-GRD or N-ras mutations might not be responsible for the progression of CML. |
