| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-122 |
Sentence |
denotes |
Modifications of low density lipoprotein induced by the interaction with human plasma glycosaminoglycan-protein complexes. |
| T2 |
123-257 |
Sentence |
denotes |
Glycosaminoglycan (GAG)-protein complexes from human plasma were separated into low charge (LC-GP) and high charge (HC-GP) components. |
| T3 |
258-349 |
Sentence |
denotes |
LC-GP and HC-GP differed with respect to GAG and protein composition and to molecular size. |
| T4 |
350-437 |
Sentence |
denotes |
The in vitro interaction of both GAG-protein complexes with human LDL was investigated. |
| T5 |
438-468 |
Sentence |
denotes |
LC-GP did not precipitate LDL. |
| T6 |
469-594 |
Sentence |
denotes |
On the contrary, HC-GP formed insoluble complexes with LDL, following a biphasic behaviour on increasing HC-GP concentration. |
| T7 |
595-734 |
Sentence |
denotes |
In the presence of a HC-GP/LDL ratio higher than 0.02 the interaction stoichiometry was shifted towards the formation of soluble complexes. |
| T8 |
735-800 |
Sentence |
denotes |
Papain treatment of HC-GP completely prevented LDL precipitation. |
| T9 |
801-919 |
Sentence |
denotes |
Moreover, the extent of HC-GP-induced precipitation of LDL was markedly reduced by the simultaneous addition of LC-GP. |
| T10 |
920-1064 |
Sentence |
denotes |
Data obtained with standard GAGs showed that heparin (HE) and chondroitin-6-sulphate (C6S) were the most effective ligands in precipitating LDL. |
| T11 |
1065-1131 |
Sentence |
denotes |
However, the shape of precipitation curves was markedly different. |
| T12 |
1132-1260 |
Sentence |
denotes |
C6S behaved similarly to HC-GP, suggesting that GAG chains could play an important role in insoluble complex formation with LDL. |
| T13 |
1261-1412 |
Sentence |
denotes |
Steady-state fluorescence anisotropy investigation indicated that HC-GP induced a significant decrease in the microviscosity of LDL hydrophobic region. |
| T14 |
1413-1510 |
Sentence |
denotes |
This effect was no longer detectable after either addition of LC-GP or papain treatment of HC-GP. |
| T15 |
1511-1654 |
Sentence |
denotes |
Differential scanning calorimetry (DSC) demonstrated that both lipid and protein components of LDL were affected by the interaction with HC-GP. |
| T16 |
1655-1835 |
Sentence |
denotes |
The temperature of irreversible thermal unfolding of apo B100 was shifted to a lower value and a second peak appeared in the region of the reversible melting of cholesterol esters. |
| T17 |
1836-2027 |
Sentence |
denotes |
Both the fluorescence anisotropy and the DSC data obtained with standard HE and C6S indicated that GAG chains were directly involved in affecting physico-chemical properties of complexed LDL. |
| T18 |
2028-2128 |
Sentence |
denotes |
These results suggest that the interaction with plasma HC-GP could modify LDL structural properties. |
| T19 |
2129-2247 |
Sentence |
denotes |
However, LC-GP is likely to act as a modulator, probably preventing the interaction between HC-GP and circulating LDL. |
