Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-111 |
Sentence |
denotes |
Isolation of chick renal mitochondrial ferredoxin active in the 25-hydroxyvitamin D3-1alpha-hydroxylase system. |
T2 |
112-377 |
Sentence |
denotes |
An iron-sulfur protein has been isolated from chick kidney mitochondria and purified (200-fold as determined enzymatically by its NADPH-cytochrome c reductase activity in the presence of adrenodoxin reductase) on DEAE-cellulose and gel filtration on Sephadex G-100. |
T3 |
378-461 |
Sentence |
denotes |
The purified protein showed an absorption peak at 411 nm with a shoulder at 460 nm. |
T4 |
462-598 |
Sentence |
denotes |
The electron paramagnetic resonance spectrum was typical of a ferredoxin-type iron-sulfur protein with g values: gx=gy-1.94 and gz=2.02. |
T5 |
599-665 |
Sentence |
denotes |
The molecular weight was estimated by gel filtration to be 12,500. |
T6 |
666-803 |
Sentence |
denotes |
When tested against anti-adrenodoxin gamma-globulin, the protein showed a precipitin line that fused completely with that of adrenodoxin. |
T7 |
804-924 |
Sentence |
denotes |
Based on these findings it is concluded that this protein is an iron-sulfur protein quite similar to adrenal ferredoxin. |
T8 |
925-1128 |
Sentence |
denotes |
In the presence of adrenoxodin reductase, NADPH, and carbon monoxide, the purified renal ferredoxin was found to be active in the reduction of cytochrome P-450 solubilized from chick kidney mitochondria. |
T9 |
1129-1299 |
Sentence |
denotes |
It was also effective in the reconstituted 25-hydroxyvitamin D3-1alpha-hydroxylase composed of the cytochrome P-450 from rachitic chick kidneys and adrenodoxin reductase. |
T10 |
1300-1427 |
Sentence |
denotes |
A ferredoxin reductase isolated from chick kidney mitochondria could replace adrenodoxin reductase in the reconstituted system. |
T11 |
1428-1669 |
Sentence |
denotes |
These results strongly support a previous conclusion that the kidney mitochondrial 25-hydroxyvitamin D3-1alpha-hydroxylation system consists of a renal ferredoxin reductase (presumably a flavoprotein), renal ferredoxin, and cytochrome P-450. |
T1 |
0-111 |
Sentence |
denotes |
Isolation of chick renal mitochondrial ferredoxin active in the 25-hydroxyvitamin D3-1alpha-hydroxylase system. |
T2 |
112-377 |
Sentence |
denotes |
An iron-sulfur protein has been isolated from chick kidney mitochondria and purified (200-fold as determined enzymatically by its NADPH-cytochrome c reductase activity in the presence of adrenodoxin reductase) on DEAE-cellulose and gel filtration on Sephadex G-100. |
T3 |
378-461 |
Sentence |
denotes |
The purified protein showed an absorption peak at 411 nm with a shoulder at 460 nm. |
T4 |
462-598 |
Sentence |
denotes |
The electron paramagnetic resonance spectrum was typical of a ferredoxin-type iron-sulfur protein with g values: gx=gy-1.94 and gz=2.02. |
T5 |
599-665 |
Sentence |
denotes |
The molecular weight was estimated by gel filtration to be 12,500. |
T6 |
666-803 |
Sentence |
denotes |
When tested against anti-adrenodoxin gamma-globulin, the protein showed a precipitin line that fused completely with that of adrenodoxin. |
T7 |
804-924 |
Sentence |
denotes |
Based on these findings it is concluded that this protein is an iron-sulfur protein quite similar to adrenal ferredoxin. |
T8 |
925-1128 |
Sentence |
denotes |
In the presence of adrenoxodin reductase, NADPH, and carbon monoxide, the purified renal ferredoxin was found to be active in the reduction of cytochrome P-450 solubilized from chick kidney mitochondria. |
T9 |
1129-1299 |
Sentence |
denotes |
It was also effective in the reconstituted 25-hydroxyvitamin D3-1alpha-hydroxylase composed of the cytochrome P-450 from rachitic chick kidneys and adrenodoxin reductase. |
T10 |
1300-1427 |
Sentence |
denotes |
A ferredoxin reductase isolated from chick kidney mitochondria could replace adrenodoxin reductase in the reconstituted system. |
T11 |
1428-1669 |
Sentence |
denotes |
These results strongly support a previous conclusion that the kidney mitochondrial 25-hydroxyvitamin D3-1alpha-hydroxylation system consists of a renal ferredoxin reductase (presumably a flavoprotein), renal ferredoxin, and cytochrome P-450. |