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PubMed:819431 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-63 Sentence denotes 3-Hydroxy-3-methylglutaryl coenzyme A reductase from rat liver.
T2 64-121 Sentence denotes Its purification, properties, and immunochemical studies.
T3 122-301 Sentence denotes 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase, EC 1.1.1.34) was purified from rat liver microsomes after solubilization by a slow freezing and thawing method.
T4 302-509 Sentence denotes The purification was accomplished by a five-step procedure involving incubation at 37 degrees, ammonium sulfate fractionation, ultrafiltration, and column chromatography on Bio-Gel A-0.5m and Sephadex G-200.
T5 510-701 Sentence denotes The specific activities of the purified enzyme preparations were up to 480 nmol of mevalonate formed/min/mg of protein, which represented an increase of 350-fold above that of the microsomes.
T6 702-797 Sentence denotes The purified enzyme was found to be essentially homogeneous as evidences by the usual criteria.
T7 798-911 Sentence denotes A subunit molecular weight of 120,000 was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T8 912-1107 Sentence denotes On gel filtration a number of different molecular weight forms were observed which seem to be influenced by temperature, method of purification, and possibly an enzyme-Bio-Gel A-0.5m interaction.
T9 1108-1283 Sentence denotes The solubilized enzyme consisted predominantly of a species with a molecular weight slightly greater than 200,000 by gel filtration and may be a dimer of two 120,000 subunits.
T10 1284-1474 Sentence denotes The purified preparation contained lipids; the total cholesterol content was 18 mug/mg of enzyme protein and corresponds to a ratio of 5 mol of cholesterol/enzyme subunit of 120,000 daltons.
T11 1475-1656 Sentence denotes The specificity of rabbit antiserum prepared against the purified enzyme was demonstrated by double diffusion analysis and quantitative precipitin reactions with solubilized enzyme.
T12 1657-1780 Sentence denotes The antiserum, in addition to inhibiting the activity of solubilized enzyme also blocked the activity of intact microsomes.
T13 1781-1935 Sentence denotes The microsomal HMG-CoA reductase is accessible to the antibody, indicating a localization of the enzyme on the outer cytoplasmic surface of the membranes.
T14 1936-2035 Sentence denotes Intestinal microsomal HMG-CoA reductase was shown to cross-react with antibody to the liver enzyme.
T1 0-63 Sentence denotes 3-Hydroxy-3-methylglutaryl coenzyme A reductase from rat liver.
T2 64-121 Sentence denotes Its purification, properties, and immunochemical studies.
T3 122-301 Sentence denotes 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase, EC 1.1.1.34) was purified from rat liver microsomes after solubilization by a slow freezing and thawing method.
T4 302-509 Sentence denotes The purification was accomplished by a five-step procedure involving incubation at 37 degrees, ammonium sulfate fractionation, ultrafiltration, and column chromatography on Bio-Gel A-0.5m and Sephadex G-200.
T5 510-701 Sentence denotes The specific activities of the purified enzyme preparations were up to 480 nmol of mevalonate formed/min/mg of protein, which represented an increase of 350-fold above that of the microsomes.
T6 702-797 Sentence denotes The purified enzyme was found to be essentially homogeneous as evidences by the usual criteria.
T7 798-911 Sentence denotes A subunit molecular weight of 120,000 was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T8 912-1107 Sentence denotes On gel filtration a number of different molecular weight forms were observed which seem to be influenced by temperature, method of purification, and possibly an enzyme-Bio-Gel A-0.5m interaction.
T9 1108-1283 Sentence denotes The solubilized enzyme consisted predominantly of a species with a molecular weight slightly greater than 200,000 by gel filtration and may be a dimer of two 120,000 subunits.
T10 1284-1474 Sentence denotes The purified preparation contained lipids; the total cholesterol content was 18 mug/mg of enzyme protein and corresponds to a ratio of 5 mol of cholesterol/enzyme subunit of 120,000 daltons.
T11 1475-1656 Sentence denotes The specificity of rabbit antiserum prepared against the purified enzyme was demonstrated by double diffusion analysis and quantitative precipitin reactions with solubilized enzyme.
T12 1657-1780 Sentence denotes The antiserum, in addition to inhibiting the activity of solubilized enzyme also blocked the activity of intact microsomes.
T13 1781-1935 Sentence denotes The microsomal HMG-CoA reductase is accessible to the antibody, indicating a localization of the enzyme on the outer cytoplasmic surface of the membranes.
T14 1936-2035 Sentence denotes Intestinal microsomal HMG-CoA reductase was shown to cross-react with antibody to the liver enzyme.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 57-62 http://purl.obolibrary.org/obo/MAT_0000097 denotes liver
T2 225-230 http://purl.obolibrary.org/obo/MAT_0000097 denotes liver
T3 1936-1946 http://purl.obolibrary.org/obo/MAT_0000043 denotes Intestinal
T4 2022-2027 http://purl.obolibrary.org/obo/MAT_0000097 denotes liver

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 57-62 Body_part denotes liver http://purl.obolibrary.org/obo/MAT_0000097
T2 225-230 Body_part denotes liver http://purl.obolibrary.org/obo/MAT_0000097
T3 1936-1946 Body_part denotes Intestinal http://purl.obolibrary.org/obo/MAT_0000043
T4 2022-2027 Body_part denotes liver http://purl.obolibrary.org/obo/MAT_0000097

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 53-56 OrganismTaxon denotes rat 10114|10116
T3 221-224 OrganismTaxon denotes rat 10114|10116

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 57-62 Body_part denotes liver http://purl.obolibrary.org/obo/UBERON_0002107
T2 225-230 Body_part denotes liver http://purl.obolibrary.org/obo/UBERON_0002107
T3 1898-1909 Body_part denotes cytoplasmic http://purl.obolibrary.org/obo/GO_0005737
T4 1925-1934 Body_part denotes membranes http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T7 2022-2027 Body_part denotes liver http://purl.obolibrary.org/obo/UBERON_0002107