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PubMed:8132520 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-22 Sentence denotes Lactate monooxygenase.
T2 23-27 Sentence denotes III.
T3 28-148 Sentence denotes Additive contributions of active site residues to catalytic efficiency and stabilization of an anionic transition state.
T4 149-275 Sentence denotes Lactate monooxygenase catalyzes the conversion of L-lactate to acetate, CO2, and water with incorporation of molecular oxygen.
T5 276-414 Sentence denotes Several amino acid residues of lactate monooxygenase had been postulated to interact in specific ways with the bound substrate (Giegel, D.
T6 415-431 Sentence denotes A., Williams, C.
T7 432-465 Sentence denotes H., Jr., and Massey, V. (1990) J.
T8 466-471 Sentence denotes Biol.
T9 472-477 Sentence denotes Chem.
T10 478-494 Sentence denotes 265, 6626-6632).
T11 495-614 Sentence denotes Tyrosine 44 and arginine 293 were proposed to form a hydrogen bond and a salt bridge to the carboxyl-moiety of lactate.
T12 615-785 Sentence denotes Tyrosine 152 was suggested to form a hydrogen bond to the alpha-hydroxyl group and could be involved in stabilizing a transient carbanionic intermediate of the substrate.
T13 786-906 Sentence denotes The tyrosine residues were replaced with phenylalanines (Y44F, Y152F), and arginine 293 was mutated to a lysine (R293K).
T14 907-1020 Sentence denotes In all cases catalysis was significantly decreased; however, the binding affinity for L-lactate did not decrease.
T15 1021-1109 Sentence denotes Instead, the Kd measured for Y152F was 10-fold lower than that for the wild type enzyme.
T16 1110-1260 Sentence denotes The products of turnover with Y152F were similar to those with wild type enzyme, with 70-80% of the reaction proceeding to form acetate, CO2, and H2O.
T17 1261-1416 Sentence denotes The catalytic reactions with both Y44F and R293K were substantially uncoupled, with between 60 and 80% of the catalytic turnover forming pyruvate and H2O2.
T18 1417-1572 Sentence denotes For all mutant forms the reoxidation of enzyme with oxygen in the absence of pyruvate occurred at a rate similar to that measured for the wild type enzyme.
T19 1573-1684 Sentence denotes The most important effect of the mutations was in the ability to stabilize the transition state analog oxalate.
T20 1685-2013 Sentence denotes A linear relationship was found between the rate of reduction of the enzyme flavin and the dissociation constant for the binding of oxalate, demonstrating that many individual residues contribute to the lowering of the energy of the transition state, in addition to specific functions being assignable to some specific residues.
T1 0-22 Sentence denotes Lactate monooxygenase.
T2 23-27 Sentence denotes III.
T3 28-148 Sentence denotes Additive contributions of active site residues to catalytic efficiency and stabilization of an anionic transition state.
T4 149-275 Sentence denotes Lactate monooxygenase catalyzes the conversion of L-lactate to acetate, CO2, and water with incorporation of molecular oxygen.
T5 276-414 Sentence denotes Several amino acid residues of lactate monooxygenase had been postulated to interact in specific ways with the bound substrate (Giegel, D.
T6 415-431 Sentence denotes A., Williams, C.
T7 432-465 Sentence denotes H., Jr., and Massey, V. (1990) J.
T8 466-471 Sentence denotes Biol.
T9 472-477 Sentence denotes Chem.
T10 478-494 Sentence denotes 265, 6626-6632).
T11 495-614 Sentence denotes Tyrosine 44 and arginine 293 were proposed to form a hydrogen bond and a salt bridge to the carboxyl-moiety of lactate.
T12 615-785 Sentence denotes Tyrosine 152 was suggested to form a hydrogen bond to the alpha-hydroxyl group and could be involved in stabilizing a transient carbanionic intermediate of the substrate.
T13 786-906 Sentence denotes The tyrosine residues were replaced with phenylalanines (Y44F, Y152F), and arginine 293 was mutated to a lysine (R293K).
T14 907-1020 Sentence denotes In all cases catalysis was significantly decreased; however, the binding affinity for L-lactate did not decrease.
T15 1021-1109 Sentence denotes Instead, the Kd measured for Y152F was 10-fold lower than that for the wild type enzyme.
T16 1110-1260 Sentence denotes The products of turnover with Y152F were similar to those with wild type enzyme, with 70-80% of the reaction proceeding to form acetate, CO2, and H2O.
T17 1261-1416 Sentence denotes The catalytic reactions with both Y44F and R293K were substantially uncoupled, with between 60 and 80% of the catalytic turnover forming pyruvate and H2O2.
T18 1417-1572 Sentence denotes For all mutant forms the reoxidation of enzyme with oxygen in the absence of pyruvate occurred at a rate similar to that measured for the wild type enzyme.
T19 1573-1684 Sentence denotes The most important effect of the mutations was in the ability to stabilize the transition state analog oxalate.
T20 1685-2013 Sentence denotes A linear relationship was found between the rate of reduction of the enzyme flavin and the dissociation constant for the binding of oxalate, demonstrating that many individual residues contribute to the lowering of the energy of the transition state, in addition to specific functions being assignable to some specific residues.