PubMed:8120391 JSONTXT

{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":51,"end":65},"obj":"protein"},{"id":"T2","span":{"begin":96,"end":102},"obj":"protein"},{"id":"T3","span":{"begin":104,"end":111},"obj":"protein"},{"id":"T4","span":{"begin":215,"end":221},"obj":"protein"},{"id":"T5","span":{"begin":273,"end":280},"obj":"protein"},{"id":"T6","span":{"begin":323,"end":329},"obj":"protein"},{"id":"T7","span":{"begin":331,"end":338},"obj":"protein"},{"id":"T8","span":{"begin":341,"end":348},"obj":"protein"},{"id":"T9","span":{"begin":365,"end":369},"obj":"protein"},{"id":"T10","span":{"begin":416,"end":422},"obj":"protein"},{"id":"T11","span":{"begin":521,"end":527},"obj":"protein"},{"id":"T12","span":{"begin":535,"end":540},"obj":"protein"},{"id":"T13","span":{"begin":558,"end":565},"obj":"protein"},{"id":"T14","span":{"begin":571,"end":578},"obj":"protein"},{"id":"T15","span":{"begin":702,"end":707},"obj":"protein"},{"id":"T16","span":{"begin":723,"end":730},"obj":"protein"},{"id":"T17","span":{"begin":859,"end":866},"obj":"protein"},{"id":"T18","span":{"begin":930,"end":933},"obj":"protein"},{"id":"T19","span":{"begin":960,"end":967},"obj":"protein"},{"id":"T20","span":{"begin":1005,"end":1012},"obj":"protein"},{"id":"T21","span":{"begin":1014,"end":1021},"obj":"protein"},{"id":"T22","span":{"begin":1049,"end":1053},"obj":"protein"},{"id":"T23","span":{"begin":1063,"end":1068},"obj":"protein"},{"id":"T24","span":{"begin":1103,"end":1112},"obj":"protein"}],"text":"Expression cloning and characterization of a human IL-10 receptor.\ncDNA clones encoding a human IL-10R (hIL-10R) from a Burkitt lymphoma cell line, BJAB, express a 90 to 110 kDa polypeptide in COS7 cells that binds hIL-10 specifically. The predicted amino acid sequence of hIL-10R is 60% identical and 73% similar to mouse IL-10R (mIL-10R). rIL-10R expressed in an IL-3-dependent mouse pro-B cell line (Ba/F3) binds hIL-10 with high affinity (200 to 250 pM), and the transfected cells exhibit a proliferative response to hIL-10. Mouse IL-10 does not bind to hIL-10R, and hIL-10R-expressing Ba/F3 cells do not respond to the mouse cytokine, observations consistent with the known species specificity of IL-10. Expression of hIL-10R mRNA seems to be restricted mainly to human hemopoietic cells and cell lines. In a number of human T cell clones, expression of hIL-10R mRNA is down-regulated after activation of the cells with anti-CD3 Ab and phorbol ester. The hIL-10R gene is on human chromosome 11. Like mIL-10R, hIL-10R is structurally related to IFNR. Because IL-10 inhibits macrophage activation by IFN-gamma, this relationship suggests possible shared receptor or signal transduction pathway components."}