| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-121 |
Sentence |
denotes |
Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases. |
| T2 |
122-254 |
Sentence |
denotes |
A novel member of the family of G protein-coupled receptor kinases (GRKs), named GRK5, has been cloned from bovine taste epithelium. |
| T3 |
255-356 |
Sentence |
denotes |
The cDNA sequence predicts a 590-amino acid protein with high overall similarity to rhodopsin kinase. |
| T4 |
357-504 |
Sentence |
denotes |
GRK5 mRNA is found most abundantly in lung, heart, retina, and lingual epithelium, but is expressed very little in brain, liver, kidney, or testis. |
| T5 |
505-570 |
Sentence |
denotes |
GRK5 expressed in Sf9 cells was purified to apparent homogeneity. |
| T6 |
571-641 |
Sentence |
denotes |
GRK5 major autophosphorylation sites were mapped to Ser484 and Thr485. |
| T7 |
642-844 |
Sentence |
denotes |
Purified GRK5 phosphorylates rhodopsin in a light-dependent manner and beta 2-adrenergic receptor in an agonist-dependent manner and phosphorylates the C-terminal tail regions of both receptor proteins. |
| T8 |
845-1036 |
Sentence |
denotes |
GRK5 possesses neither a CAAX motif specifying protein prenylation like rhodopsin kinase nor similarity to the G protein beta gamma-subunit binding domain of beta-adrenergic receptor kinases. |
| T9 |
1037-1152 |
Sentence |
denotes |
GRK5 phosphorylation of rhodopsin or beta 2-adrenergic receptor is not stimulated by G protein beta gamma-subunits. |
| T10 |
1153-1367 |
Sentence |
denotes |
The GRK5 protein does not undergo agonist-dependent translocation from cytosol to membranes as do beta-adrenergic receptor kinase and rhodopsin kinase, but rather appears to associate with membranes constitutively. |
| T11 |
1368-1524 |
Sentence |
denotes |
GRK5 thus appears functionally similar to other characterized GRKs, but has distinct regulatory properties which may be important for its cellular function. |
| T1 |
0-121 |
Sentence |
denotes |
Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases. |
| T2 |
122-254 |
Sentence |
denotes |
A novel member of the family of G protein-coupled receptor kinases (GRKs), named GRK5, has been cloned from bovine taste epithelium. |
| T3 |
255-356 |
Sentence |
denotes |
The cDNA sequence predicts a 590-amino acid protein with high overall similarity to rhodopsin kinase. |
| T4 |
357-504 |
Sentence |
denotes |
GRK5 mRNA is found most abundantly in lung, heart, retina, and lingual epithelium, but is expressed very little in brain, liver, kidney, or testis. |
| T5 |
505-570 |
Sentence |
denotes |
GRK5 expressed in Sf9 cells was purified to apparent homogeneity. |
| T6 |
571-641 |
Sentence |
denotes |
GRK5 major autophosphorylation sites were mapped to Ser484 and Thr485. |
| T7 |
642-844 |
Sentence |
denotes |
Purified GRK5 phosphorylates rhodopsin in a light-dependent manner and beta 2-adrenergic receptor in an agonist-dependent manner and phosphorylates the C-terminal tail regions of both receptor proteins. |
| T8 |
845-1036 |
Sentence |
denotes |
GRK5 possesses neither a CAAX motif specifying protein prenylation like rhodopsin kinase nor similarity to the G protein beta gamma-subunit binding domain of beta-adrenergic receptor kinases. |
| T9 |
1037-1152 |
Sentence |
denotes |
GRK5 phosphorylation of rhodopsin or beta 2-adrenergic receptor is not stimulated by G protein beta gamma-subunits. |
| T10 |
1153-1367 |
Sentence |
denotes |
The GRK5 protein does not undergo agonist-dependent translocation from cytosol to membranes as do beta-adrenergic receptor kinase and rhodopsin kinase, but rather appears to associate with membranes constitutively. |
| T11 |
1368-1524 |
Sentence |
denotes |
GRK5 thus appears functionally similar to other characterized GRKs, but has distinct regulatory properties which may be important for its cellular function. |