Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-121 |
Sentence |
denotes |
Purification and characterization of eukaryotic initiation factor (eIF)-2 alpha kinases from Ehrlich ascites tumor cells. |
T2 |
122-296 |
Sentence |
denotes |
A major mechanism of regulation of mammalian protein synthesis initiation is accomplished by the phosphorylation of the alpha subunit of eukaryotic initiation factor (eIF) 2. |
T3 |
297-542 |
Sentence |
denotes |
This modification inhibits the activity of another initiation factor, guanine nucleotide exchange factor, preventing conversion of eIF-2.GDP to eIF-2.GTP and hence binding of initiator tRNA and formation of ternary complex (eIF-2.GTP.Met-tRNAf). |
T4 |
543-749 |
Sentence |
denotes |
Inhibition of protein synthesis and phosphorylation of eIF-2 occurs in Ehrlich cells when they are amino acid- or serum-deprived or heat-shocked as well as in other nucleated cells under similar conditions. |
T5 |
750-834 |
Sentence |
denotes |
This paper describes the purification of two eIF-2 alpha kinases from Ehrlich cells. |
T6 |
835-1082 |
Sentence |
denotes |
Unlike the two well characterized eIF-2 alpha kinases, HRI (heme-regulated inhibitor from reticulocytes) and P68 (double-stranded RNA-dependent kinase found in interferon-treated cells), the Ehrlich cell kinases do not appear to autophosphorylate. |
T7 |
1083-1154 |
Sentence |
denotes |
The two kinases chromatograph differently on DEAE-cellulose and Mono Q. |
T8 |
1155-1228 |
Sentence |
denotes |
Furthermore, their Michaelis constants (Km values) for ATP are different. |
T9 |
1229-1347 |
Sentence |
denotes |
Both kinases can inhibit purified guanine nucleotide exchange factor (GEF) from stimulating ternary complex formation. |
T10 |
1348-1430 |
Sentence |
denotes |
However, only one kinase inhibits reticulocyte lysate cell free protein synthesis. |
T11 |
1431-1569 |
Sentence |
denotes |
The other kinase co-purifies with a factor that suppresses inhibition of protein synthesis in reticulocyte lysates by eIF-2 alpha kinases. |
T12 |
1570-1643 |
Sentence |
denotes |
This suppressing activity is probably guanine nucleotide exchange factor. |
T1 |
0-121 |
Sentence |
denotes |
Purification and characterization of eukaryotic initiation factor (eIF)-2 alpha kinases from Ehrlich ascites tumor cells. |
T2 |
122-296 |
Sentence |
denotes |
A major mechanism of regulation of mammalian protein synthesis initiation is accomplished by the phosphorylation of the alpha subunit of eukaryotic initiation factor (eIF) 2. |
T3 |
297-542 |
Sentence |
denotes |
This modification inhibits the activity of another initiation factor, guanine nucleotide exchange factor, preventing conversion of eIF-2.GDP to eIF-2.GTP and hence binding of initiator tRNA and formation of ternary complex (eIF-2.GTP.Met-tRNAf). |
T4 |
543-749 |
Sentence |
denotes |
Inhibition of protein synthesis and phosphorylation of eIF-2 occurs in Ehrlich cells when they are amino acid- or serum-deprived or heat-shocked as well as in other nucleated cells under similar conditions. |
T5 |
750-834 |
Sentence |
denotes |
This paper describes the purification of two eIF-2 alpha kinases from Ehrlich cells. |
T6 |
835-1082 |
Sentence |
denotes |
Unlike the two well characterized eIF-2 alpha kinases, HRI (heme-regulated inhibitor from reticulocytes) and P68 (double-stranded RNA-dependent kinase found in interferon-treated cells), the Ehrlich cell kinases do not appear to autophosphorylate. |
T7 |
1083-1154 |
Sentence |
denotes |
The two kinases chromatograph differently on DEAE-cellulose and Mono Q. |
T8 |
1155-1228 |
Sentence |
denotes |
Furthermore, their Michaelis constants (Km values) for ATP are different. |
T9 |
1229-1347 |
Sentence |
denotes |
Both kinases can inhibit purified guanine nucleotide exchange factor (GEF) from stimulating ternary complex formation. |
T10 |
1348-1430 |
Sentence |
denotes |
However, only one kinase inhibits reticulocyte lysate cell free protein synthesis. |
T11 |
1431-1569 |
Sentence |
denotes |
The other kinase co-purifies with a factor that suppresses inhibition of protein synthesis in reticulocyte lysates by eIF-2 alpha kinases. |
T12 |
1570-1643 |
Sentence |
denotes |
This suppressing activity is probably guanine nucleotide exchange factor. |