Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-65 |
Sentence |
denotes |
Ribonucleoside diphosphate reductase induced by bacteriophage T4. |
T2 |
66-70 |
Sentence |
denotes |
III. |
T3 |
71-124 |
Sentence |
denotes |
Isolation and characterization of proteins B1 and B2. |
T4 |
125-314 |
Sentence |
denotes |
Ribonucleoside diphosphate reductase determined by bacteriophage T4 consists of a tight complex (alpha2beta2) of the polypeptide chains alpha (Mr = 80,000 to 85,000) and beta (Mr = 35,000). |
T5 |
315-554 |
Sentence |
denotes |
The alpha2 dimer (= protein B1) was purified from Escherichia coli B infected with T4 mutant nrdB55 (Yeh, Y.C., and Tessman, I. (1972) Virology 47, 767-772) which carries an amber mutation in the gene coding for the beta polypeptide chain. |
T6 |
555-640 |
Sentence |
denotes |
Protein B1 contained binding sites for dATP, an allosteric effector of the reductase. |
T7 |
641-825 |
Sentence |
denotes |
The beta2 dimer (= protein B2) was purified by selective desorption with 1 M guanidine HCl from a dATP-Sepharose affinity column containing adsorbed native T4 ribonucleotide reductase. |
T8 |
826-975 |
Sentence |
denotes |
Protein B2, isolated this way, was enzymatically inactive due to partial loss of its iron but it could be reactivated by treatment with ferrous iron. |
T9 |
976-1151 |
Sentence |
denotes |
Active protein B2 contained two atoms of non-heme iron per molecule and exhibited the optical and electron spin resonance spectra previously demonstrated in the native enzyme. |
T10 |
1152-1279 |
Sentence |
denotes |
The T4-induced proteins B1 and B2 were unable to reduce ribonucleotides when assayed separately but were active in combination. |
T11 |
1280-1470 |
Sentence |
denotes |
The proteins did not form catalytically functional hybrids with proteins B1 and B2 of Escherichia coli ribonucleotide reductase, neither did they cross-react immunologically with the latter. |
T12 |
1471-1661 |
Sentence |
denotes |
5-Hydroxymethyl-dCTP, at concentrations above 10 muM, was a positive allosteric effector of T4 ribonucleotide reductase promoting the reduction of the pyrimidine ribonucleotides CDP and UDP. |
T13 |
1662-1731 |
Sentence |
denotes |
The nucleotide had little effect on E. coli ribonucleotide reductase. |
T1 |
0-65 |
Sentence |
denotes |
Ribonucleoside diphosphate reductase induced by bacteriophage T4. |
T2 |
66-70 |
Sentence |
denotes |
III. |
T3 |
71-124 |
Sentence |
denotes |
Isolation and characterization of proteins B1 and B2. |
T4 |
125-314 |
Sentence |
denotes |
Ribonucleoside diphosphate reductase determined by bacteriophage T4 consists of a tight complex (alpha2beta2) of the polypeptide chains alpha (Mr = 80,000 to 85,000) and beta (Mr = 35,000). |
T5 |
315-554 |
Sentence |
denotes |
The alpha2 dimer (= protein B1) was purified from Escherichia coli B infected with T4 mutant nrdB55 (Yeh, Y.C., and Tessman, I. (1972) Virology 47, 767-772) which carries an amber mutation in the gene coding for the beta polypeptide chain. |
T6 |
555-640 |
Sentence |
denotes |
Protein B1 contained binding sites for dATP, an allosteric effector of the reductase. |
T7 |
641-825 |
Sentence |
denotes |
The beta2 dimer (= protein B2) was purified by selective desorption with 1 M guanidine HCl from a dATP-Sepharose affinity column containing adsorbed native T4 ribonucleotide reductase. |
T8 |
826-975 |
Sentence |
denotes |
Protein B2, isolated this way, was enzymatically inactive due to partial loss of its iron but it could be reactivated by treatment with ferrous iron. |
T9 |
976-1151 |
Sentence |
denotes |
Active protein B2 contained two atoms of non-heme iron per molecule and exhibited the optical and electron spin resonance spectra previously demonstrated in the native enzyme. |
T10 |
1152-1279 |
Sentence |
denotes |
The T4-induced proteins B1 and B2 were unable to reduce ribonucleotides when assayed separately but were active in combination. |
T11 |
1280-1470 |
Sentence |
denotes |
The proteins did not form catalytically functional hybrids with proteins B1 and B2 of Escherichia coli ribonucleotide reductase, neither did they cross-react immunologically with the latter. |
T12 |
1471-1661 |
Sentence |
denotes |
5-Hydroxymethyl-dCTP, at concentrations above 10 muM, was a positive allosteric effector of T4 ribonucleotide reductase promoting the reduction of the pyrimidine ribonucleotides CDP and UDP. |
T13 |
1662-1731 |
Sentence |
denotes |
The nucleotide had little effect on E. coli ribonucleotide reductase. |